Restriction map for pF1KF0175
##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp718I     
AvrII       BcgI        BciVI       BclI        BfrI        
BlnI        BsiWI       BsmI        Bsp1407I    BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
CpoI        DraI        EcoRI       EcoRV       EcoT22I     
FbaI        HpaI        KpnI        KspI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SapI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SwaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        2770  GACGTC               GACGT^C
AccII         851  CGCG                 CG^CG
AflIII       3524  ACATGT               A^C[GA][CT]GT
AhdI         4471  GACGGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1812  CCTTAGG              CC^T[ACGT]AGG
Aor51HI      4035  AGCGCT               AGC^GCT
AspEI        4471  GACGGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        5407  CACGTG               CAC^GTG
BbvCI        2665  GCTGAGG              CCTCAGC(-5/-2)
BsaAI        5407  CACGTG               [CT]AC^GT[GA]
BsaI         1735  GAGACC               GGTCTC(1/5)
BspHI        4524  TCATGA               T^CATGA
BspLU11I     3524  ACATGT               A^CATGT
BsrDI        5772  CATTGC               GCAATG(2/0)
BstUI         851  CGCG                 CG^CG
Bsu36I       1812  CCTTAGG              CC^T[ACGT]AGG
DraIII       4373  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4146  GACGGTGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2816  CGGCCG               C^GGCCG
Eam1105I     4471  GACGGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2816  CGGCCG               C^GGCCG
Eco47III     4035  AGCGCT               AGC^GCT
Eco52I       2816  CGGCCG               C^GGCCG
Eco81I       1812  CCTTAGG              CC^T[ACGT]AGG
FseI          539  GGCCGGCC             GGCCGG^CC
HindIII      6157  AAGCTT               A^AGCTT
MvnI          851  CGCG                 CG^CG
NcoI          509  CCATGG               C^CATGG
PaeR7I       3295  CTCGAG               C^TCGAG
PmaCI        5407  CACGTG               CAC^GTG
PmlI         5407  CACGTG               CAC^GTG
RcaI         4524  TCATGA               T^CATGA
SbfI          357  CCTGCAGG             CCTGCA^GG
ScaI         1826  AGTACT               AGT^ACT
SphI         5909  GCATGC               GCATG^C
Sse8387I      357  CCTGCAGG             CCTGCA^GG
SspI         5297  AATATT               AAT^ATT
StuI         4655  AGGCCT               AGG^CCT
XbaI          721  TCTAGA               T^CTAGA
XhoI         3295  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AviII        2282  TGCGCA               TGC^GCA
             3188  TGCGCA              
BfaI          287  CTAG                 C^TAG
              722  CTAG                
BsaBI        1476  GATTCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3675  GATCGAGATC          
BsiEI        2816  CGGCCG               CG[GA][CT]^CG
             5102  CGGTCG              
BspDI        4270  ATCGAT               AT^CGAT
             4540  ATCGAT              
BstEII        822  GGTTACC              G^GT[ACGT]ACC
             2887  GGTCACC             
BstPI         822  GGTTACC              G^GT[ACGT]ACC
             2887  GGTCACC             
ClaI         4270  ATCGAT               AT^CGAT
             4540  ATCGAT              
EcoO65I       822  GGTTACC              G^GT[ACGT]ACC
             2887  GGTCACC             
FspI         2282  TGCGCA               TGC^GCA
             3188  TGCGCA              
MaeI          287  CTAG                 C^TAG
              722  CTAG                
MamI         1476  GATTCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3675  GATCGAGATC          
MseI         3638  TTAA                 T^TAA
             4751  TTAA                
PshAI        2881  GACGCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5100  GACGGTCGTC          
SalI         5107  GTCGAC               G^TCGAC
             5476  GTCGAC              
Tru9I        3638  TTAA                 T^TAA
             4751  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccI        AccIII      AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApaI        
ApoI        Asp700I     AspHI       AspI        AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspEI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        XmnI        
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