Restriction map for pF1KF0175
 (Linear) (Six-base) MAPSORT of: FLJ00175.seq  Check: 6858  from: 1  to: 6357



FLJ00175.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     351    6357 
   Size:        351    6006
 
AatII G_ACGT'C

Cuts at:      0    2774    6357 
   Size:       2774    3583
 
AccI GT'mk_AC

Cuts at:      0     956    5108    5477    6357 
   Size:        956    4152     369     880

  Fragments arranged by size: 

                4152     956     880     369
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1190    1264    2161    2347    4642    5365    6336    6357 
   Size:       1190      74     897     186    2295     723     971      21

  Fragments arranged by size: 

                2295    1190     971     897     723     186      74      21
            
 
AfeI AGC'GCT

Cuts at:      0    4037    6357 
   Size:       4037    2320
 
AflIII A'CryG_T

Cuts at:      0    3524    6357 
   Size:       3524    2833
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4476    6357 
   Size:       4476    1881
 
AleI CACnn'nnGTG

Cuts at:      0    2974    6357 
   Size:       2974    3383
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1072    2223    2271    3706    5394    6357 
   Size:       1072    1151      48    1435    1688     963

  Fragments arranged by size: 

                1688    1435    1151    1072     963      48
 
ApaI G_GGCC'C

Cuts at:      0    1613    3399    5350    6357 
   Size:       1613    1786    1951    1007

  Fragments arranged by size: 

                1951    1786    1613    1007
 
ApoI r'AATT_y

Cuts at:      0    4555    4747    4766    6357 
   Size:       4555     192      19    1591

  Fragments arranged by size: 

                4555    1591     192      19
 
AvaI C'yCGr_G

Cuts at:      0     633     688     988    3295    3536    6357 
   Size:        633      55     300    2307     241    2821

  Fragments arranged by size: 

                2821    2307     633     300     241      55
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    5245    5278    6357 
   Size:       5245      33    1079

  Fragments arranged by size: 

                5245    1079      33
 
BamHI G'GATC_C

Cuts at:      0     854    3944    5327    6357 
   Size:        854    3090    1383    1030

  Fragments arranged by size: 

                3090    1383    1030     854
 
BanI G'GyrC_C

Cuts at:      0     782    1012    1985    2478    2512    2580    2587    3335 
   Size:        782     230     973     493      34      68       7     748

Cuts at:   3335    5253    6173    6357 
   Size:       1918     920     184

  Fragments arranged by size: 

                1918     973     920     782     748     493     230     184
                  68      34       7
 
BanII G_rGCy'C

Cuts at:      0     190     425     642     938    1031    1233    1613    1679 
   Size:        190     235     217     296      93     202     380      66

Cuts at:   1679    2201    2348    2411    2633    3399    3881    4241    4448 
   Size:        522     147      63     222     766     482     360     207

Cuts at:   4448    4991    5145    5350    5887    6143    6357 
   Size:        543     154     205     537     256     214

  Fragments arranged by size: 

                 766     543     537     522     482     380     360     296
                 256     235     222     217     214     207     205     202
                 190     154     147      93      66      63
 
BbeI G_GCGC'C

Cuts at:      0     786    2591    3339    6177    6357 
   Size:        786    1805     748    2838     180

  Fragments arranged by size: 

                2838    1805     786     748     180
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     575    1178    1242    1408    2866    3367    3619    4421 
   Size:        575     603      64     166    1458     501     252     802

Cuts at:   4421    4790    6357 
   Size:        369    1567

  Fragments arranged by size: 

                1567    1458     802     603     575     501     369     252
                 166      64
 
BbvCI CC'TCA_GC

Cuts at:      0    2666    6357 
   Size:       2666    3691
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2143    3023    4286    6357 
   Size:       2143     880    1263    2071

  Fragments arranged by size: 

                2143    2071    1263     880
 
BglII A'GATC_T

Cuts at:      0    1307    1391    3680    4928    5843    6357 
   Size:       1307      84    2289    1248     915     514

  Fragments arranged by size: 

                2289    1307    1248     915     514      84
 
BlpI GC'TnA_GC

Cuts at:      0    2183    3707    5756    6357 
   Size:       2183    1524    2049     601

  Fragments arranged by size: 

                2183    2049    1524     601
 
Bme1580I G_kGCm'C

Cuts at:      0     395    1015    1613    2397    2483    2740    3019    3399 
   Size:        395     620     598     784      86     257     279     380

Cuts at:   3399    5350    6357 
   Size:       1951    1007

  Fragments arranged by size: 

                1951    1007     784     620     598     395     380     279
                 257      86
 
BmgBI CAC'GTC

Cuts at:      0     670    1008    2166    4419    5682    6357 
   Size:        670     338    1158    2253    1263     675

  Fragments arranged by size: 

                2253    1263    1158     675     670     338
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      32    1500    2055    2742    3031    3141    5344    6357 
   Size:         32    1468     555     687     289     110    2203    1013

  Fragments arranged by size: 

                2203    1468    1013     687     555     289     110      32
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     292    2262    3285    6357 
   Size:        292    1970    1023    3072

  Fragments arranged by size: 

                3072    1970    1023     292
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     439     614    1171    1580    1753    2140    2380 
   Size:        404      35     175     557     409     173     387     240

Cuts at:   2380    2855    3376    4024    4348    4621    4663    5005    5053 
   Size:        475     521     648     324     273      42     342      48

Cuts at:   5053    5134    5742    5814    6357 
   Size:         81     608      72     543

  Fragments arranged by size: 

                 648     608     557     543     521     475     409     404
                 387     342     324     273     240     175     173      81
                  72      48      42      35
 
Bpu10I CC'TnA_GC

Cuts at:      0    2296    2666    2893    5599    6155    6357 
   Size:       2296     370     227    2706     556     202

  Fragments arranged by size: 

                2706    2296     556     370     227     202
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148    3409    3471    6181    6357 
   Size:        148    3261      62    2710     176

  Fragments arranged by size: 

                3261    2710     176     148      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1729    6357 
   Size:       1729    4628
 
BsaAI yAC'GTr

Cuts at:      0    5409    6357 
   Size:       5409     948
 
BsaBI GATnn'nnATC

Cuts at:      0    1480    3679    6357 
   Size:       1480    2199    2678

  Fragments arranged by size: 

                2678    2199    1480
 
BsaHI Gr'CG_yC

Cuts at:      0     783    1880    2588    2771    2882    3336    6174    6357 
   Size:        783    1097     708     183     111     454    2838     183

  Fragments arranged by size: 

                2838    1097     783     708     454     183     183     111
            
 
BsaWI w'CCGG_w

Cuts at:      0     998    2853    4175    5264    6245    6357 
   Size:        998    1855    1322    1089     981     112

  Fragments arranged by size: 

                1855    1322    1089     998     981     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    4096    4126    4491    4521    5059    5089    5848    5878 
   Size:       4096      30     365      30     538      30     759      30

Cuts at:   5878    6357 
   Size:        479

  Fragments arranged by size: 

                4096     759     538     479     365      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     404     575     976    1241    1577    2419    4101    4249 
   Size:        404     171     401     265     336     842    1682     148

Cuts at:   4249    4499    4732    5062    5209    5371    6124    6357 
   Size:        250     233     330     147     162     753     233

  Fragments arranged by size: 

                1682     842     753     404     401     336     330     265
                 250     233     233     171     162     148     147
 
BseYI C'CCAG_C

Cuts at:      0    1087    1125    3086    3176    3257    3718    4117    4788 
   Size:       1087      38    1961      90      81     461     399     671

Cuts at:   4788    5213    5823    6357 
   Size:        425     610     534

  Fragments arranged by size: 

                1961    1087     671     610     534     461     425     399
                  90      81      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1404    1991    2623    3583    3779    3949    4171    4185 
   Size:       1404     587     632     960     196     170     222      14

Cuts at:   4185    4962    5004    6357 
   Size:        777      42    1353

  Fragments arranged by size: 

                1404    1353     960     777     632     587     222     196
                 170      42      14
 
BsiEI CG_ry'CG

Cuts at:      0    2819    5105    6357 
   Size:       2819    2286    1252

  Fragments arranged by size: 

                2819    2286    1252
 
BsiHKAI G_wGCw'C

Cuts at:      0    1679    1754    2155    2201    2348    3881    4241    4424 
   Size:       1679      75     401      46     147    1533     360     183

Cuts at:   4424    5408    5944    6357 
   Size:        984     536     413

  Fragments arranged by size: 

                1679    1533     984     536     413     401     360     183
                 147      75      46
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     214    3447    3594    5092    6357 
   Size:        214    3233     147    1498    1265

  Fragments arranged by size: 

                3233    1498    1265     214     147
 
Bsp1286I G_dGCh'C

Cuts at:      0     190     395     425     642     938    1015    1031    1233 
   Size:        190     205      30     217     296      77      16     202

Cuts at:   1233    1613    1679    1754    2155    2201    2348    2397    2411 
   Size:        380      66      75     401      46     147      49      14

Cuts at:   2411    2483    2633    2740    3019    3399    3881    4241    4424 
   Size:         72     150     107     279     380     482     360     183

Cuts at:   4424    4448    4991    5145    5350    5408    5887    5944    6143 
   Size:         24     543     154     205      58     479      57     199

Cuts at:   6143    6357 
   Size:        214

  Fragments arranged by size: 

                 543     482     479     401     380     380     360     296
                 279     217     214     205     205     202     199     190
                 183     154     150     147     107      77      75      72
                  66      58      57      49      46      30      24      16
                  14
 
BspEI T'CCGG_A

Cuts at:      0     998    2853    4175    6357 
   Size:        998    1855    1322    2182

  Fragments arranged by size: 

                2182    1855    1322     998
 
BspHI T'CATG_A

Cuts at:      0    4524    6357 
   Size:       4524    1833
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     351    1205    1942    2115    3980    5026    5756    6357 
   Size:        351     854     737     173    1865    1046     730     601

  Fragments arranged by size: 

                1865    1046     854     737     730     601     351     173
            
 
BsrBI CCG'CTC

Cuts at:      0    3117    4322    6114    6357 
   Size:       3117    1205    1792     243

  Fragments arranged by size: 

                3117    1792    1205     243
 
BsrDI GCAATG_nn'

Cuts at:      0    5771    6357 
   Size:       5771     586
 
BsrFI r'CCGG_y

Cuts at:      0      53     540    1715    2102    2818    2884    3693    3897 
   Size:         53     487    1175     387     716      66     809     204

Cuts at:   3897    4292    6357 
   Size:        395    2065

  Fragments arranged by size: 

                2065    1175     809     716     487     395     387     204
                  66      53
 
BssSI C'ACGA_G

Cuts at:      0     516    4123    5828    6357 
   Size:        516    3607    1705     529

  Fragments arranged by size: 

                3607    1705     529     516
 
BstEII G'GTnAC_C

Cuts at:      0     822    2887    6357 
   Size:        822    2065    3470

  Fragments arranged by size: 

                3470    2065     822
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1746    2089    2259    3026    3726    3916    4125    6339 
   Size:       1746     343     170     767     700     190     209    2214

Cuts at:   6339    6357 
   Size:         18

  Fragments arranged by size: 

                2214    1746     767     700     343     209     190     170
                  18
 
BstYI r'GATC_y

Cuts at:      0     854    1307    1391    2948    3198    3680    3944    4728 
   Size:        854     453      84    1557     250     482     264     784

Cuts at:   4728    4928    5327    5843    6357 
   Size:        200     399     516     514

  Fragments arranged by size: 

                1557     854     784     516     514     482     453     399
                 264     250     200      84
 
Bsu36I CC'TnA_GG

Cuts at:      0    1813    6357 
   Size:       1813    4544
 
BtgI C'CryG_G

Cuts at:      0     509     548    2156    3811    5530    6357 
   Size:        509      39    1608    1655    1719     827

  Fragments arranged by size: 

                1719    1655    1608     827     509      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2187    3565    6357 
   Size:       2187    1378    2792

  Fragments arranged by size: 

                2792    2187    1378
 
BtsI GCAGTG_nn'

Cuts at:      0    1435    2155    6357 
   Size:       1435     720    4202

  Fragments arranged by size: 

                4202    1435     720
 
ClaI AT'CG_AT

Cuts at:      0    4271    4541    6357 
   Size:       4271     270    1816

  Fragments arranged by size: 

                4271    1816     270
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2505    2517    2540    2552    6357 
   Size:       2505      12      23      12    3805

  Fragments arranged by size: 

                3805    2505      23      12      12
 
DraIII CAC_nnn'GTG

Cuts at:      0    4378    6357 
   Size:       4378    1979
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    4152    6357 
   Size:       4152    2205
 
EaeI y'GGCC_r

Cuts at:      0     538    1406    1713    2334    2816    3695    3899    4100 
   Size:        538     868     307     621     482     879     204     201

Cuts at:   4100    4287    4826    6123    6296    6357 
   Size:        187     539    1297     173      61

  Fragments arranged by size: 

                1297     879     868     621     539     538     482     307
                 204     201     187     173      61
 
EagI C'GGCC_G

Cuts at:      0    2816    6357 
   Size:       2816    3541
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     883    2503    2900    4081    4213    4526    4765 
   Size:         94     789    1620     397    1181     132     313     239

Cuts at:   4765    5233    5303    6032    6268    6280    6357 
   Size:        468      70     729     236      12      77

  Fragments arranged by size: 

                1620    1181     789     729     468     397     313     239
                 236     132      94      77      70      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     285     619     665    2383    2797    3253    3259    3310 
   Size:        285     334      46    1718     414     456       6      51

Cuts at:   3310    4372    5194    6357 
   Size:       1062     822    1163

  Fragments arranged by size: 

                1718    1163    1062     822     456     414     334     285
                  51      46       6
 
EcoICRI GAG'CTC

Cuts at:      0    1677    2199    2346    3879    4239    6357 
   Size:       1677     522     147    1533     360    2118

  Fragments arranged by size: 

                2118    1677    1533     522     360     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     439     614    1171    1190    1264    1580    1753 
   Size:        404      35     175     557      19      74     316     173

Cuts at:   1753    2140    2161    2347    2380    2855    3376    4024    4348 
   Size:        387      21     186      33     475     521     648     324

Cuts at:   4348    4621    4642    4663    5005    5053    5134    5365    5742 
   Size:        273      21      21     342      48      81     231     377

Cuts at:   5742    5814    6336    6357 
   Size:         72     522      21

  Fragments arranged by size: 

                 648     557     522     521     475     404     387     377
                 342     324     316     273     231     186     175     173
                  81      74      72      48      35      33      21      21
                  21      21      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     181    1044    2122    2401    4603    5077    5793    6357 
   Size:        181     863    1078     279    2202     474     716     564

  Fragments arranged by size: 

                2202    1078     863     716     564     474     279     181
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159     258     273     344    1129    1610    1773    3135 
   Size:        159      99      15      71     785     481     163    1362

Cuts at:   3135    3367    3395    3396    3540    4071    4665    4755    4837 
   Size:        232      28       1     144     531     594      90      82

Cuts at:   4837    4896    5040    5391    5493    6165    6357 
   Size:         59     144     351     102     672     192

  Fragments arranged by size: 

                1362     785     672     594     531     481     351     232
                 192     163     159     144     144     102      99      90
                  82      71      59      28      15       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      98    1353    2820    4776    5805    5883    5961    6357 
   Size:         98    1255    1467    1956    1029      78      78     396

  Fragments arranged by size: 

                1956    1467    1255    1029     396      98      78      78
            
 
FseI GG_CCGG'CC

Cuts at:      0     544    6357 
   Size:        544    5813
 
FspI TGC'GCA

Cuts at:      0    2284    3190    6357 
   Size:       2284     906    3167

  Fragments arranged by size: 

                3167    2284     906
 
HaeII r_GCGC'y

Cuts at:      0     786    1147    2591    3177    3339    4039    6177    6357 
   Size:        786     361    1444     586     162     700    2138     180

  Fragments arranged by size: 

                2138    1444     786     700     586     361     180     162
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     472     504     966     998    1066    1098 
   Size:        172      32     268      32     462      32      68      32

Cuts at:   1098    2155    2187    3034    3066    3421    3453    3547    3579 
   Size:       1057      32     847      32     355      32      94      32

Cuts at:   3579    4336    4351    4368    4383    4462    4494    4654    4686 
   Size:        757      15      17      15      79      32     160      32

Cuts at:   4686    4713    4745    5007    5039    5059    5091    5570    5602 
   Size:         27      32     262      32      20      32     479      32

Cuts at:   5602    5848    5880    6357 
   Size:        246      32     477

  Fragments arranged by size: 

                1057     847     757     479     477     462     355     268
                 262     246     172     160      94      79      68      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      27      20
                  17      15      15
 
HincII GTy'rAC

Cuts at:      0    3790    5109    5478    6357 
   Size:       3790    1319     369     879

  Fragments arranged by size: 

                3790    1319     879     369
 
HindIII A'AGCT_T

Cuts at:      0    6157    6357 
   Size:       6157     200
 
KasI G'GCGC_C

Cuts at:      0     782    2587    3335    6173    6357 
   Size:        782    1805     748    2838     184

  Fragments arranged by size: 

                2838    1805     782     748     184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    5623    6357 
   Size:       5623     734
 
MscI TGG'CCA

Cuts at:      0    4102    6125    6298    6357 
   Size:       4102    2023     173      59

  Fragments arranged by size: 

                4102    2023     173      59
 
MslI CAynn'nnrTG

Cuts at:      0    2440    2974    3727    4009    4216    4798    5632    6357 
   Size:       2440     534     753     282     207     582     834     725

  Fragments arranged by size: 

                2440     834     753     725     582     534     282     207
            
 
MspA1I CmG'CkG

Cuts at:      0     378     789     829    1944    2085    2187    2280    2475 
   Size:        378     411      40    1115     141     102      93     195

Cuts at:   2475    3332    3376    4098    4641    4824    5755    5955    6357 
   Size:        857      44     722     543     183     931     200     402

  Fragments arranged by size: 

                1115     931     857     722     543     411     402     378
                 200     195     183     141     102      93      44      40
            
 
NaeI GCC'GGC

Cuts at:      0     542    1717    2820    3695    3899    4294    6357 
   Size:        542    1175    1103     875     204     395    2063

  Fragments arranged by size: 

                2063    1175    1103     875     542     395     204
 
NarI GG'CG_CC

Cuts at:      0     783    2588    3336    6174    6357 
   Size:        783    1805     748    2838     183

  Fragments arranged by size: 

                2838    1805     783     748     183
 
NcoI C'CATG_G

Cuts at:      0     509    6357 
   Size:        509    5848
 
NgoMIV G'CCGG_C

Cuts at:      0     540    1715    2818    3693    3897    4292    6357 
   Size:        540    1175    1103     875     204     395    2065

  Fragments arranged by size: 

                2065    1175    1103     875     540     395     204
 
NspI r_CATG'y

Cuts at:      0    3528    5377    5913    6357 
   Size:       3528    1849     536     444

  Fragments arranged by size: 

                3528    1849     536     444
 
PasI CC'CwG_GG

Cuts at:      0    2006    3399    4229    4833    5496    5697    6357 
   Size:       2006    1393     830     604     663     201     660

  Fragments arranged by size: 

                2006    1393     830     663     660     604     201
 
PciI A'CATG_T

Cuts at:      0    3524    6357 
   Size:       3524    2833
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2223    2877    4378    4824    6357 
   Size:       2223     654    1501     446    1533

  Fragments arranged by size: 

                2223    1533    1501     654     446
 
PfoI T'CCnGG_A

Cuts at:      0     614    2934    3947    5399    5588    6357 
   Size:        614    2320    1013    1452     189     769

  Fragments arranged by size: 

                2320    1452    1013     769     614     189
 
PmlI CAC'GTG

Cuts at:      0    5409    6357 
   Size:       5409     948
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2635    2667    4519    4551    6357 
   Size:       2635      32    1852      32    1806

  Fragments arranged by size: 

                2635    1852    1806      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     159     258     273    1773    3135    3540    4837    4896 
   Size:        159      99      15    1500    1362     405    1297      59

Cuts at:   4896    5040    6165    6357 
   Size:        144    1125     192

  Fragments arranged by size: 

                1500    1362    1297    1125     405     192     159     144
                  99      59      15
 
PshAI GACnn'nnGTC

Cuts at:      0    2885    5104    6357 
   Size:       2885    2219    1253

  Fragments arranged by size: 

                2885    2219    1253
 
PspOMI G'GGCC_C

Cuts at:      0    1609    3395    5346    6357 
   Size:       1609    1786    1951    1011

  Fragments arranged by size: 

                1951    1786    1609    1011
 
PspXI vC'TCGA_Gb

Cuts at:      0    3295    6357 
   Size:       3295    3062
 
PstI C_TGCA'G

Cuts at:      0     362     834    1868    2215    2355    2450    3375    4889 
   Size:        362     472    1034     347     140      95     925    1514

Cuts at:   4889    5441    5468    5894    6357 
   Size:        552      27     426     463

  Fragments arranged by size: 

                1514    1034     925     552     472     463     426     362
                 347     140      95      27
 
PvuII CAG'CTG

Cuts at:      0     789    2085    2187    2280    3376    4098    4641    4824 
   Size:        789    1296     102      93    1096     722     543     183

Cuts at:   4824    5755    5955    6357 
   Size:        931     200     402

  Fragments arranged by size: 

                1296    1096     931     789     722     543     402     200
                 183     102      93
 
SacI G_AGCT'C

Cuts at:      0    1679    2201    2348    3881    4241    6357 
   Size:       1679     522     147    1533     360    2116

  Fragments arranged by size: 

                2116    1679    1533     522     360     147
 
SalI G'TCGA_C

Cuts at:      0    5107    5476    6357 
   Size:       5107     369     881

  Fragments arranged by size: 

                5107     881     369
 
SanDI GG'GwC_CC

Cuts at:      0    3135    4837    6357 
   Size:       3135    1702    1520

  Fragments arranged by size: 

                3135    1702    1520
 
SbfI CC_TGCA'GG

Cuts at:      0     362    6357 
   Size:        362    5995
 
ScaI AGT'ACT

Cuts at:      0    1828    6357 
   Size:       1828    4529
 
SfcI C'TryA_G

Cuts at:      0     358     830    1864    2211    2351    2446    2545    3371 
   Size:        358     472    1034     347     140      95      99     826

Cuts at:   3371    4885    5427    5437    5464    5890    6357 
   Size:       1514     542      10      27     426     467

  Fragments arranged by size: 

                1514    1034     826     542     472     467     426     358
                 347     140      99      95      27      10
 
SfoI GGC'GCC

Cuts at:      0     784    2589    3337    6175    6357 
   Size:        784    1805     748    2838     182

  Fragments arranged by size: 

                2838    1805     784     748     182
 
SmaI CCC'GGG

Cuts at:      0     635     690    3538    6357 
   Size:        635      55    2848    2819

  Fragments arranged by size: 

                2848    2819     635      55
 
SmlI C'TyrA_G

Cuts at:      0     163    3295    3388    3450    6160    6357 
   Size:        163    3132      93      62    2710     197

  Fragments arranged by size: 

                3132    2710     197     163      93      62
 
SphI G_CATG'C

Cuts at:      0    5913    6357 
   Size:       5913     444
 
SspI AAT'ATT

Cuts at:      0    5299    6357 
   Size:       5299    1058
 
StuI AGG'CCT

Cuts at:      0    4657    6357 
   Size:       4657    1700
 
StyI C'CwwG_G

Cuts at:      0     254     509     652    2795    2999    4892    5078    5945 
   Size:        254     255     143    2143     204    1893     186     867

Cuts at:   5945    6080    6169    6357 
   Size:        135      89     188

  Fragments arranged by size: 

                2143    1893     867     255     254     204     188     186
                 143     135      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2215    2269    2302    3168    5196    6357 
   Size:       2215      54      33     866    2028    1161

  Fragments arranged by size: 

                2215    2028    1161     866      54      33
 
TatI w'GTAC_w

Cuts at:      0    1826    1961    3152    6357 
   Size:       1826     135    1191    3205

  Fragments arranged by size: 

                3205    1826    1191     135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4491    4523    5259    5291    6357 
   Size:       4491      32     736      32    1066

  Fragments arranged by size: 

                4491    1066     736      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      67     486    2431    6357 
   Size:         67     419    1945    3926

  Fragments arranged by size: 

                3926    1945     419      67
 
XbaI T'CTAG_A

Cuts at:      0     721    6357 
   Size:        721    5636
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     589    2163    3726    3751    3818    3913    4008    4059 
   Size:        589    1574    1563      25      67      95      95      51

Cuts at:   4059    4323    6357 
   Size:        264    2034

  Fragments arranged by size: 

                2034    1574    1563     589     264      95      95      67
                  51      25
 
XhoI C'TCGA_G

Cuts at:      0    3295    6357 
   Size:       3295    3062
 
XmaI C'CCGG_G

Cuts at:      0     633     688    3536    6357 
   Size:        633      55    2848    2821

  Fragments arranged by size: 

                2848    2821     633      55
 
XmnI GAAnn'nnTTC

Cuts at:      0     238    1249    1921    2650    6349    6357 
   Size:        238    1011     672     729    3699       8

  Fragments arranged by size: 

                3699    1011     729     672     238       8
 
ZraI GAC'GTC

Cuts at:      0    2772    6357 
   Size:       2772    3585
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI     SalI    SanDI     SbfI     ScaI     SfcI     SfoI
     SmaI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AlfI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BcgI    BciVI     BclI    BfrBI     BmtI
    BsiWI     BsmI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I     DraI
    EcoRI    EcoRV    FspAI     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PsiI     PsrI
     PvuI    RsrII    SacII     SapI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SwaI
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