Restriction map for pF1KB9466
 (Linear) (Six-base) MAPSORT of: KIBB9466.seq  Check: 414  from: 1  to: 2331



KIBB9466.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:      0     394     550    2331 
   Size:        394     156    1781

  Fragments arranged by size: 

                1781     394     156
 
AclI AA'CG_TT

Cuts at:      0    1570    2331 
   Size:       1570     761
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476    1268    1429    1643    1951    2003    2059    2331 
   Size:        476     792     161     214     308      52      56     272

  Fragments arranged by size: 

                 792     476     308     272     214     161      56      52
            
 
AflIII A'CryG_T

Cuts at:      0    1691    2331 
   Size:       1691     640
 
AleI CACnn'nnGTG

Cuts at:      0     436    2331 
   Size:        436    1895
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1730    2331 
   Size:       1730     601
 
ApoI r'AATT_y

Cuts at:      0    1400    1496    2242    2331 
   Size:       1400      96     746      89

  Fragments arranged by size: 

                1400     746      96      89
 
AseI AT'TA_AT

Cuts at:      0    1886    2331 
   Size:       1886     445
 
AvaI C'yCGr_G

Cuts at:      0     339    2248    2331 
   Size:        339    1909      83

  Fragments arranged by size: 

                1909     339      83
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     976    1009    2331 
   Size:        976      33    1322

  Fragments arranged by size: 

                1322     976      33
 
BanI G'GyrC_C

Cuts at:      0     523    1097    2331 
   Size:        523     574    1234

  Fragments arranged by size: 

                1234     574     523
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     122     183     698    1435    2331 
   Size:        122      61     515     737     896

  Fragments arranged by size: 

                 896     737     515     122      61
 
BfrBI ATG'CAT

Cuts at:      0    2175    2331 
   Size:       2175     156
 
BglI GCCn_nnn'nGGC

Cuts at:      0     521    2331 
   Size:        521    1810
 
BglII A'GATC_T

Cuts at:      0     228     783    2331 
   Size:        228     555    1548

  Fragments arranged by size: 

                1548     555     228
 
Bme1580I G_kGCm'C

Cuts at:      0     895    2331 
   Size:        895    1436
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     797     898    2331 
   Size:        797     101    1433

  Fragments arranged by size: 

                1433     797     101
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1919    2331 
   Size:       1919     412
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     941    1001    1130    2106    2331 
   Size:        941      60     129     976     225

  Fragments arranged by size: 

                 976     941     225     129      60
 
Bpu10I CC'TnA_GC

Cuts at:      0      59    2331 
   Size:         59    2272
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1188    2331 
   Size:       1188    1143
 
BsaWI w'CCGG_w

Cuts at:      0     750    2331 
   Size:        750    1581
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      98     128     773     803    2331 
   Size:         98      30     645      30    1528

  Fragments arranged by size: 

                1528     645      98      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     116     119     668    1202    1215    2331 
   Size:        116       3     549     534      13    1116

  Fragments arranged by size: 

                1116     549     534     116      13       3
 
BseYI C'CCAG_C

Cuts at:      0      43    1186    1419    2331 
   Size:         43    1143     233     912

  Fragments arranged by size: 

                1143     912     233      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     843    2331 
   Size:        843    1488
 
BsiHKAI G_wGCw'C

Cuts at:      0    1273    2331 
   Size:       1273    1058
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     613    2331 
   Size:        613    1718
 
Bsp1286I G_dGCh'C

Cuts at:      0     895    1273    2331 
   Size:        895     378    1058

  Fragments arranged by size: 

                1058     895     378
 
BspHI T'CATG_A

Cuts at:      0    1676    2331 
   Size:       1676     655
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1865    2331 
   Size:       1865     466
 
BsrDI GCAATG_nn'

Cuts at:      0     219     374     543    2331 
   Size:        219     155     169    1788

  Fragments arranged by size: 

                1788     219     169     155
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     433    2331 
   Size:        433    1898
 
BstBI TT'CG_AA

Cuts at:      0    1404    2331 
   Size:       1404     927
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1108    2331 
   Size:       1108    1223
 
BstYI r'GATC_y

Cuts at:      0     228     783    1105    2331 
   Size:        228     555     322    1226

  Fragments arranged by size: 

                1226     555     322     228
 
BstZ17I GTA'TAC

Cuts at:      0     551    2331 
   Size:        551    1780
 
Bsu36I CC'TnA_GG

Cuts at:      0    2027    2331 
   Size:       2027     304
 
BtsI GCAGTG_nn'

Cuts at:      0      54     431     814    1349    1727    2331 
   Size:         54     377     383     535     378     604

  Fragments arranged by size: 

                 604     535     383     378     377      54
 
ClaI AT'CG_AT

Cuts at:      0    1394    2331 
   Size:       1394     937
 
EarI CTCTTCn'nnn_

Cuts at:      0    1976    2022    2035    2331 
   Size:       1976      46      13     296

  Fragments arranged by size: 

                1976     296      46      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     941    1001    1130    1268    1429    1643    1951 
   Size:        476     465      60     129     138     161     214     308

Cuts at:   1951    2003    2059    2106    2331 
   Size:         52      56      47     225

  Fragments arranged by size: 

                 476     465     308     225     214     161     138     129
                  60      56      52      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     605    2331 
   Size:        605    1726
 
EcoRI G'AATT_C

Cuts at:      0    1496    2242    2331 
   Size:       1496     746      89

  Fragments arranged by size: 

                1496     746      89
 
FspI TGC'GCA

Cuts at:      0     218    2331 
   Size:        218    2113
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1192    1224    2331 
   Size:       1192      32    1107

  Fragments arranged by size: 

                1192    1107      32
 
HincII GTy'rAC

Cuts at:      0     395    2331 
   Size:        395    1936
 
HindIII A'AGCT_T

Cuts at:      0     358     912    1280    2331 
   Size:        358     554     368    1051

  Fragments arranged by size: 

                1051     554     368     358
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1586    2331 
   Size:       1586     745
 
MslI CAynn'nnrTG

Cuts at:      0     436     482    1766    2331 
   Size:        436      46    1284     565

  Fragments arranged by size: 

                1284     565     436      46
 
MspA1I CmG'CkG

Cuts at:      0    2139    2331 
   Size:       2139     192
 
NsiI A_TGCA'T

Cuts at:      0    2177    2331 
   Size:       2177     154
 
NspI r_CATG'y

Cuts at:      0    1427    1695    1917    1938    2331 
   Size:       1427     268     222      21     393

  Fragments arranged by size: 

                1427     393     268     222      21
 
PasI CC'CwG_GG

Cuts at:      0     865    2331 
   Size:        865    1466
 
PciI A'CATG_T

Cuts at:      0    1691    2331 
   Size:       1691     640
 
PfoI T'CCnGG_A

Cuts at:      0     918    1795    2331 
   Size:        918     877     536

  Fragments arranged by size: 

                 918     877     536
 
PpuMI rG'GwC_Cy

Cuts at:      0     605    2331 
   Size:        605    1726
 
PstI C_TGCA'G

Cuts at:      0    1790    2331 
   Size:       1790     541
 
SalI G'TCGA_C

Cuts at:      0     393    2331 
   Size:        393    1938
 
SanDI GG'GwC_CC

Cuts at:      0     605    2331 
   Size:        605    1726
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2035    2331 
   Size:       2035     296
 
ScaI AGT'ACT

Cuts at:      0    1792    2331 
   Size:       1792     539
 
SexAI A'CCwGG_T

Cuts at:      0    1743    2331 
   Size:       1743     588
 
SfcI C'TryA_G

Cuts at:      0    1511    1786    2331 
   Size:       1511     275     545

  Fragments arranged by size: 

                1511     545     275
 
SphI G_CATG'C

Cuts at:      0    1427    2331 
   Size:       1427     904
 
SspI AAT'ATT

Cuts at:      0    2290    2331 
   Size:       2290      41
 
StyI C'CwwG_G

Cuts at:      0     634    1101    2331 
   Size:        634     467    1230

  Fragments arranged by size: 

                1230     634     467
 
TatI w'GTAC_w

Cuts at:      0    1790    2331 
   Size:       1790     541
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      98     130    2331 
   Size:         98      32    2201

  Fragments arranged by size: 

                2201      98      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1260    1664    2331 
   Size:       1260     404     667

  Fragments arranged by size: 

                1260     667     404
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AleI     AlfI     ApoI     AseI
     AvaI     BaeI     BanI     BbsI    BfrBI     BglI    BglII Bme1580I
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI
     EarI  Eco57MI EcoO109I    EcoRI     FspI    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NsiI     NspI     PasI     PciI     PfoI
    PpuMI     PstI     SalI    SanDI     SapI     ScaI    SexAI     SfcI
     SphI     SspI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AloI
    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BlpI    BmgBI     BmtI
    BpuEI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstEII     BtgI    BtgZI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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