Restriction map for pF1KB9389
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       Bsu36I      
CelII       CfoI        ClaI        CpoI        DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HhaI        HinP1I      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI         1546  AAATTT               [GA]^AATT[CT]
AcyI          658  GACGCC               G[GA]^CG[CT]C
AfaI          975  GTAC                 GT^AC
AgeI         1091  ACCGGT               A^CCGGT
AhdI          658  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         1546  AAATTT               [GA]^AATT[CT]
AspEI         658  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         877  GAGCTC               G[AT]GC[AT]^C
AvaI          832  CTCGAG               C^[CT]CG[GA]G
BanI          856  GGCACC               G^G[CT][GA]CC
BciVI         810  GGATAC               GTATCC(6/5)
BclI          822  TGATCA               T^GATCA
BglII        1929  AGATCT               A^GATCT
BsaHI         658  GACGCC               G[GA]^CG[CT]C
BsiHKAI       877  GAGCTC               G[AT]GC[AT]^C
BsoBI         832  CTCGAG               C^[CT]CG[GA]G
BspHI         141  TCATGA               T^CATGA
BspMI        1890  ACCTGC               ACCTGC(4/8)
BsrBI         888  CCGCTC               CCGCTC(-3/-3)
DraI          738  TTTAAA               TTT^AAA
DraII         495  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1628  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          992  CCACGG               C^C[GA][CT]GG
Eam1105I      658  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1411  GAAGAG               CTCTTC(1/4)
EcoO109I      495  AGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI          822  TGATCA               T^GATCA
HgaI          658  GACGC                GACGC(5/10)
Hin1I         658  GACGCC               G[GA]^CG[CT]C
HincII        120  GTCAAC               GT[CT]^[GA]AC
HindII        120  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1411  GAAGAG               CTCTTC(1/4)
MaeII         526  ACGT                 A^CGT
NaeI         1224  GCCGGC               GCC^GGC
NgoMIV       1224  GCCGGC               G^CCGGC
PaeR7I        832  CTCGAG               C^TCGAG
PinAI        1091  ACCGGT               A^CCGGT
PleI         1453  GAGTC                GAGTC(4/5)
PpuMI         495  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1936  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1892  CTGCAG               CTGCA^G
RcaI          141  TCATGA               T^CATGA
RsaI          975  GTAC                 GT^AC
SacI          877  GAGCTC               GAGCT^C
SmlI          832  CTCGAG               C^T[CT][GA]AG
XhoI          832  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          528  GTATAC               GT^[AC][GT]AC
             1202  GTATAC              
AciI          888  CCGC                 CCGC(-3/-1)
             1151  CCGC                
AlwI          550  GATCC                GGATC(4/5)
             1300  GATCC               
Asp700I      1384  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1462  GAACAGCTTC          
BanII         877  GAGCTC               G[GA]GC[CT]^C
             1622  GAGCCC              
BbsI         1354  GAAGAC               GAAGAC(2/6)
             1483  GAAGAC              
BcnI          370  CCGGG                CC^[GC]GG
              860  CCCGG               
BfaI          934  CTAG                 C^TAG
             1693  CTAG                
BpmI         1157  CTCCAG               CTGGAG(16/14)
             1619  CTGGAG              
BpuAI        1354  GAAGAC               GAAGAC(2/6)
             1483  GAAGAC              
BsaWI         429  ACCGGA               [AT]^CCGG[AT]
             1091  ACCGGT              
BsmAI        1772  GAGAC                GTCTC(1/5)
             1182  GTCTC               
BsmFI         757  GGGAC                GGGAC(10/14)
             1943  GTCCC               
BsrFI        1091  ACCGGT               [GA]^CCGG[CT]
             1224  GCCGGC              
Bst1107I      528  GTATAC               GTA^TAC
             1202  GTATAC              
BstNI        1281  CCTGG                CC^[AT]GG
             1824  CCTGG               
BstZ17I       528  GTATAC               GTA^TAC
             1202  GTATAC              
Cfr10I       1091  ACCGGT               [GA]^CCGG[CT]
             1224  GCCGGC              
EcoRII       1281  CCTGG                ^CC[AT]GG
             1824  CCTGG               
HindIII      1366  AAGCTT               A^AGCTT
             1777  AAGCTT              
MaeI          934  CTAG                 C^TAG
             1693  CTAG                
MvaI         1281  CCTGG                CC^[AT]GG
             1824  CCTGG               
NciI          370  CCGGG                CC^[GC]GG
              860  CCCGG               
NlaIV         856  GGCACC               GG[ACGT]^[ACGT]CC
             1621  GGAGCC              
PflMI          36  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1303  CCAGAAGATGG         
Van91I         36  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1303  CCAGAAGATGG         
XcmI          125  CCAAGCTGTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1574  CCATCTCTGAAGTGG     
XmnI         1384  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1462  GAACAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AluI        AlwNI       AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MwoI        
NdeII       NlaIII      PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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