Restriction map for pF1KB9389
 (Linear) (Six-base) MAPSORT of: KIBB9389.seq  Check: 9512  from: 1  to: 2085



KIBB9389.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:      0     529    1203    2085 
   Size:        529     674     882

  Fragments arranged by size: 

                 882     674     529
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     530    1049    1601    2085 
   Size:        521       9     519     552     484

  Fragments arranged by size: 

                 552     521     519     484       9
 
AgeI A'CCGG_T

Cuts at:      0    1091    2085 
   Size:       1091     994
 
AhdI GACnn_n'nnGTC

Cuts at:      0     663    2085 
   Size:        663    1422
 
AlwNI CAG_nnn'CTG

Cuts at:      0     103     700     874    1027    1165    2085 
   Size:        103     597     174     153     138     920

  Fragments arranged by size: 

                 920     597     174     153     138     103
 
ApoI r'AATT_y

Cuts at:      0    1546    2085 
   Size:       1546     539
 
AvaI C'yCGr_G

Cuts at:      0     832    2085 
   Size:        832    1253
 
BanI G'GyrC_C

Cuts at:      0     856    2085 
   Size:        856    1229
 
BanII G_rGCy'C

Cuts at:      0     881    1626    2085 
   Size:        881     745     459

  Fragments arranged by size: 

                 881     745     459
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1361    1490    2085 
   Size:       1361     129     595

  Fragments arranged by size: 

                1361     595     129
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     804    2085 
   Size:        804    1281
 
BclI T'GATC_A

Cuts at:      0     822    2085 
   Size:        822    1263
 
BglII A'GATC_T

Cuts at:      0    1929    2085 
   Size:       1929     156
 
Bme1580I G_kGCm'C

Cuts at:      0     859    1243    2085 
   Size:        859     384     842

  Fragments arranged by size: 

                 859     842     384
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     862    2085 
   Size:        862    1223
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1533    2085 
   Size:       1533     552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1142    1640    2085 
   Size:       1142     498     445

  Fragments arranged by size: 

                1142     498     445
 
Bpu10I CC'TnA_GC

Cuts at:      0     875    1220    2085 
   Size:        875     345     865

  Fragments arranged by size: 

                 875     865     345
 
BsaHI Gr'CG_yC

Cuts at:      0     659    2085 
   Size:        659    1426
 
BsaWI w'CCGG_w

Cuts at:      0     429    1091    2085 
   Size:        429     662     994

  Fragments arranged by size: 

                 994     662     429
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     323     353     588     618    1165    1195    1377    1407 
   Size:        323      30     235      30     547      30     182      30

Cuts at:   1407    2085 
   Size:        678

  Fragments arranged by size: 

                 678     547     323     235     182      30      30      30
                  30
 
BseYI C'CCAG_C

Cuts at:      0     405    1599    2085 
   Size:        405    1194     486

  Fragments arranged by size: 

                1194     486     405
 
BsiHKAI G_wGCw'C

Cuts at:      0     881    2085 
   Size:        881    1204
 
Bsp1286I G_dGCh'C

Cuts at:      0     859     881    1243    1626    2085 
   Size:        859      22     362     383     459

  Fragments arranged by size: 

                 859     459     383     362      22
 
BspHI T'CATG_A

Cuts at:      0     141    2085 
   Size:        141    1944
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1899    2085 
   Size:       1899     186
 
BsrBI CCG'CTC

Cuts at:      0     890    2085 
   Size:        890    1195
 
BsrFI r'CCGG_y

Cuts at:      0    1091    1224    2085 
   Size:       1091     133     861

  Fragments arranged by size: 

                1091     861     133
 
BstYI r'GATC_y

Cuts at:      0     549    1299    1929    2085 
   Size:        549     750     630     156

  Fragments arranged by size: 

                 750     630     549     156
 
BstZ17I GTA'TAC

Cuts at:      0     530    1204    2085 
   Size:        530     674     881

  Fragments arranged by size: 

                 881     674     530
 
BtgI C'CryG_G

Cuts at:      0     992    2085 
   Size:        992    1093
 
BtsI GCAGTG_nn'

Cuts at:      0     350    2085 
   Size:        350    1735
 
DraI TTT'AAA

Cuts at:      0     740    2085 
   Size:        740    1345
 
DraIII CAC_nnn'GTG

Cuts at:      0    1633    2085 
   Size:       1633     452
 
EarI CTCTTCn'nnn_

Cuts at:      0    1406    2085 
   Size:       1406     679
 
EcoICRI GAG'CTC

Cuts at:      0     879    2085 
   Size:        879    1206
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     530    1049    1142    1601    1640    2085 
   Size:        521       9     519      93     459      39     445

  Fragments arranged by size: 

                 521     519     459     445      93      39       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     496    2085 
   Size:        496    1589
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1507    2085 
   Size:       1507     578
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129     136     168     424     456     649     681 
   Size:         97      32       7      32     256      32     193      32

Cuts at:    681     826     858     874     906    1099    1131    1165    1197 
   Size:        145      32      16      32     193      32      34      32

Cuts at:   1197    1489    1521    1666    1698    1906    1938    2085 
   Size:        292      32     145      32     208      32     147

  Fragments arranged by size: 

                 292     256     208     193     193     147     145     145
                  97      34      32      32      32      32      32      32
                  32      32      32      32      32      16       7
 
HincII GTy'rAC

Cuts at:      0     122    2085 
   Size:        122    1963
 
HindIII A'AGCT_T

Cuts at:      0    1366    1777    2085 
   Size:       1366     411     308

  Fragments arranged by size: 

                1366     411     308
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1151    2085 
   Size:       1151     934
 
MspA1I CmG'CkG

Cuts at:      0     262     409     697    1153    1940    2085 
   Size:        262     147     288     456     787     145

  Fragments arranged by size: 

                 787     456     288     262     147     145
 
NaeI GCC'GGC

Cuts at:      0    1226    2085 
   Size:       1226     859
 
NgoMIV G'CCGG_C

Cuts at:      0    1224    2085 
   Size:       1224     861
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42    1309    2085 
   Size:         42    1267     776

  Fragments arranged by size: 

                1267     776      42
 
PfoI T'CCnGG_A

Cuts at:      0    1823    2085 
   Size:       1823     262
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     863     895    2085 
   Size:        863      32    1190

  Fragments arranged by size: 

                1190     863      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     496    2085 
   Size:        496    1589
 
PshAI GACnn'nnGTC

Cuts at:      0    1940    2085 
   Size:       1940     145
 
PspXI vC'TCGA_Gb

Cuts at:      0     832    2085 
   Size:        832    1253
 
PstI C_TGCA'G

Cuts at:      0    1896    2085 
   Size:       1896     189
 
PvuII CAG'CTG

Cuts at:      0     262     409     697    1940    2085 
   Size:        262     147     288    1243     145

  Fragments arranged by size: 

                1243     288     262     147     145
 
SacI G_AGCT'C

Cuts at:      0     881    2085 
   Size:        881    1204
 
SfcI C'TryA_G

Cuts at:      0     653     692     728    1259    1892    1952    2085 
   Size:        653      39      36     531     633      60     133

  Fragments arranged by size: 

                 653     633     531     133      60      39      36
 
SmlI C'TyrA_G

Cuts at:      0     832    2085 
   Size:        832    1253
 
StyI C'CwwG_G

Cuts at:      0      17    1268    1670    2085 
   Size:         17    1251     402     415

  Fragments arranged by size: 

                1251     415     402      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1488    2085 
   Size:       1488     597
 
TatI w'GTAC_w

Cuts at:      0     974    2085 
   Size:        974    1111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     588     620    1304    1336    2085 
   Size:        588      32     684      32     749

  Fragments arranged by size: 

                 749     684     588      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132    1581    2085 
   Size:        132    1449     504

  Fragments arranged by size: 

                1449     504     132
 
XhoI C'TCGA_G

Cuts at:      0     832    2085 
   Size:        832    1253
 
XmnI GAAnn'nnTTC

Cuts at:      0    1388    1466    2085 
   Size:       1388      78     619

  Fragments arranged by size: 

                1388     619      78
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AhdI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI    BciVI     BclI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BstYI  BstZ17I     BtgI     BtsI
     DraI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I
   HincII  HindIII     MmeI   MspA1I     NaeI   NgoMIV    PflMI     PfoI
     PpiI    PpuMI    PshAI    PspXI     PstI    PvuII     SacI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI     BglI     BlpI
    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I    BtgZI     ClaI
    CspCI     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XmaI     ZraI
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