Restriction map for pF1KB4094
 (Linear) (Six-base) MAPSORT of: KIBB4094.seq  Check: 8369  from: 1  to: 1845



KIBB4094.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1259    1845 
   Size:       1259     586
 
AccI GT'mk_AC

Cuts at:      0     302    1845 
   Size:        302    1543
 
Acc65I G'GTAC_C

Cuts at:      0     839    1845 
   Size:        839    1006
 
AclI AA'CG_TT

Cuts at:      0     170    1845 
   Size:        170    1675
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     414    1162    1622    1845 
   Size:         28     386     748     460     223

  Fragments arranged by size: 

                 748     460     386     223      28
 
AflIII A'CryG_T

Cuts at:      0     977    1845 
   Size:        977     868
 
AleI CACnn'nnGTG

Cuts at:      0    1303    1845 
   Size:       1303     542
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     709     741    1845 
   Size:        709      32    1104

  Fragments arranged by size: 

                1104     709      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     516     801    1845 
   Size:        516     285    1044

  Fragments arranged by size: 

                1044     516     285
 
ApaI G_GGCC'C

Cuts at:      0     414     578    1462    1845 
   Size:        414     164     884     383

  Fragments arranged by size: 

                 884     414     383     164
 
AvaI C'yCGr_G

Cuts at:      0     175     320    1439    1592    1728    1845 
   Size:        175     145    1119     153     136     117

  Fragments arranged by size: 

                1119     175     153     145     136     117
 
AvrII C'CTAG_G

Cuts at:      0    1344    1845 
   Size:       1344     501
 
BanI G'GyrC_C

Cuts at:      0     295     460     839    1201    1528    1623    1845 
   Size:        295     165     379     362     327      95     222

  Fragments arranged by size: 

                 379     362     327     295     222     165      95
 
BanII G_rGCy'C

Cuts at:      0     182     414     454     578     887    1462    1490    1845 
   Size:        182     232      40     124     309     575      28     355

  Fragments arranged by size: 

                 575     355     309     232     182     124      40      28
            
 
BbeI G_GCGC'C

Cuts at:      0     299    1845 
   Size:        299    1546
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      28    1088    1845 
   Size:         28    1060     757

  Fragments arranged by size: 

                1060     757      28
 
BbvCI CC'TCA_GC

Cuts at:      0     385     596     936    1845 
   Size:        385     211     340     909

  Fragments arranged by size: 

                 909     385     340     211
 
BglI GCCn_nnn'nGGC

Cuts at:      0     458     511     658    1030    1405    1845 
   Size:        458      53     147     372     375     440

  Fragments arranged by size: 

                 458     440     375     372     147      53
 
BglII A'GATC_T

Cuts at:      0     867    1091    1845 
   Size:        867     224     754

  Fragments arranged by size: 

                 867     754     224
 
BlpI GC'TnA_GC

Cuts at:      0     662    1845 
   Size:        662    1183
 
Bme1580I G_kGCm'C

Cuts at:      0     414     578    1204    1462    1531    1845 
   Size:        414     164     626     258      69     314

  Fragments arranged by size: 

                 626     414     314     258     164      69
 
BmgBI CAC'GTC

Cuts at:      0      54    1845 
   Size:         54    1791
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     448    1845 
   Size:        448    1397
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     796     901    1183    1210    1845 
   Size:        321     475     105     282      27     635

  Fragments arranged by size: 

                 635     475     321     282     105      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     385     596     936    1619    1845 
   Size:        385     211     340     683     226

  Fragments arranged by size: 

                 683     385     340     226     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1807    1845 
   Size:       1807      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     366     526    1845 
   Size:        366     160    1319

  Fragments arranged by size: 

                1319     366     160
 
BsaAI yAC'GTr

Cuts at:      0    1314    1845 
   Size:       1314     531
 
BsaHI Gr'CG_yC

Cuts at:      0     296    1845 
   Size:        296    1549
 
BsaWI w'CCGG_w

Cuts at:      0     478    1845 
   Size:        478    1367
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     709     739    1845 
   Size:        709      30    1106

  Fragments arranged by size: 

                1106     709      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     889    1102    1189    1300    1432    1498    1845 
   Size:        103     786     213      87     111     132      66     347

  Fragments arranged by size: 

                 786     347     213     132     111     103      87      66
            
 
BseYI C'CCAG_C

Cuts at:      0      78     120     193    1319    1845 
   Size:         78      42      73    1126     526

  Fragments arranged by size: 

                1126     526      78      73      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745    1294    1357    1562    1789    1845 
   Size:        745     549      63     205     227      56

  Fragments arranged by size: 

                 745     549     227     205      63      56
 
BsiEI CG_ry'CG

Cuts at:      0    1183    1409    1845 
   Size:       1183     226     436

  Fragments arranged by size: 

                1183     436     226
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     182    1667    1845 
   Size:        107      75    1485     178

  Fragments arranged by size: 

                1485     178     107      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     182     414     454     578     887    1204    1462 
   Size:        107      75     232      40     124     309     317     258

Cuts at:   1462    1490    1531    1667    1845 
   Size:         28      41     136     178

  Fragments arranged by size: 

                 317     309     258     232     178     136     124     107
                  75      41      40      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     767    1145    1259    1845 
   Size:        767     378     114     586

  Fragments arranged by size: 

                 767     586     378     114
 
BsrBI CCG'CTC

Cuts at:      0     392     666    1179    1845 
   Size:        392     274     513     666

  Fragments arranged by size: 

                 666     513     392     274
 
BsrDI GCAATG_nn'

Cuts at:      0     929    1017    1845 
   Size:        929      88     828

  Fragments arranged by size: 

                 929     828      88
 
BsrFI r'CCGG_y

Cuts at:      0     652     896    1448    1531    1845 
   Size:        652     244     552      83     314

  Fragments arranged by size: 

                 652     552     314     244      83
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     801    1377    1845 
   Size:        801     576     468

  Fragments arranged by size: 

                 801     576     468
 
BstEII G'GTnAC_C

Cuts at:      0     600    1845 
   Size:        600    1245
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1161    1845 
   Size:       1161     684
 
BstYI r'GATC_y

Cuts at:      0     287     867    1091    1845 
   Size:        287     580     224     754

  Fragments arranged by size: 

                 754     580     287     224
 
Bsu36I CC'TnA_GG

Cuts at:      0     852    1845 
   Size:        852     993
 
BtgI C'CryG_G

Cuts at:      0     236     845    1423    1462    1845 
   Size:        236     609     578      39     383

  Fragments arranged by size: 

                 609     578     383     236      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     240    1845 
   Size:        240    1605
 
BtsI GCAGTG_nn'

Cuts at:      0    1550    1845 
   Size:       1550     295
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1314    1349    1845 
   Size:       1314      35     496

  Fragments arranged by size: 

                1314     496      35
 
EaeI y'GGCC_r

Cuts at:      0     239     894     898    1046    1180    1406    1533    1712 
   Size:        239     655       4     148     134     226     127     179

Cuts at:   1712    1845 
   Size:        133

  Fragments arranged by size: 

                 655     239     226     179     148     134     133     127
                   4
 
EagI C'GGCC_G

Cuts at:      0    1180    1406    1845 
   Size:       1180     226     439

  Fragments arranged by size: 

                1180     439     226
 
EarI CTCTTCn'nnn_

Cuts at:      0     433    1845 
   Size:        433    1412
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1054    1209    1845 
   Size:       1054     155     636

  Fragments arranged by size: 

                1054     636     155
 
EcoICRI GAG'CTC

Cuts at:      0     180    1845 
   Size:        180    1665
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     321     414     796     901    1162    1183    1210 
   Size:         28     293      93     382     105     261      21      27

Cuts at:   1210    1622    1845 
   Size:        412     223

  Fragments arranged by size: 

                 412     382     293     261     223     105      93      28
                  27      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     323     547    1141    1845 
   Size:        323     224     594     704

  Fragments arranged by size: 

                 704     594     323     224
 
EcoO109I rG'GnC_Cy

Cuts at:      0     503     513    1230    1458    1521    1552    1597    1845 
   Size:        503      10     717     228      63      31      45     248

  Fragments arranged by size: 

                 717     503     248     228      63      45      31      10
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1122    1845 
   Size:       1122     723
 
FseI GG_CCGG'CC

Cuts at:      0     900    1845 
   Size:        900     945
 
FspI TGC'GCA

Cuts at:      0    1381    1845 
   Size:       1381     464
 
FspAI rTGC'GCAy

Cuts at:      0    1381    1845 
   Size:       1381     464
 
HaeII r_GCGC'y

Cuts at:      0     299    1736    1845 
   Size:        299    1437     109

  Fragments arranged by size: 

                1437     299     109
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     284     316     936     968    1845 
   Size:        284      32     620      32     877

  Fragments arranged by size: 

                 877     620     284      32      32
 
HincII GTy'rAC

Cuts at:      0     150     303    1845 
   Size:        150     153    1542

  Fragments arranged by size: 

                1542     153     150
 
KasI G'GCGC_C

Cuts at:      0     295    1845 
   Size:        295    1550
 
KpnI G_GTAC'C

Cuts at:      0     843    1845 
   Size:        843    1002
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1845 
   Size:        427    1418
 
MscI TGG'CCA

Cuts at:      0    1714    1845 
   Size:       1714     131
 
MslI CAynn'nnrTG

Cuts at:      0    1303    1845 
   Size:       1303     542
 
MspA1I CmG'CkG

Cuts at:      0      36     595     789    1845 
   Size:         36     559     194    1056

  Fragments arranged by size: 

                1056     559     194      36
 
NaeI GCC'GGC

Cuts at:      0     898    1450    1845 
   Size:        898     552     395

  Fragments arranged by size: 

                 898     552     395
 
NarI GG'CG_CC

Cuts at:      0     296    1845 
   Size:        296    1549
 
NcoI C'CATG_G

Cuts at:      0     845    1845 
   Size:        845    1000
 
NdeI CA'TA_TG

Cuts at:      0     373    1845 
   Size:        373    1472
 
NgoMIV G'CCGG_C

Cuts at:      0     896    1448    1845 
   Size:        896     552     397

  Fragments arranged by size: 

                 896     552     397
 
NspI r_CATG'y

Cuts at:      0     699     981    1845 
   Size:        699     282     864

  Fragments arranged by size: 

                 864     699     282
 
PasI CC'CwG_GG

Cuts at:      0     516    1845 
   Size:        516    1329
 
PciI A'CATG_T

Cuts at:      0     977    1845 
   Size:        977     868
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     516    1845 
   Size:        516    1329
 
PfoI T'CCnGG_A

Cuts at:      0     524    1058    1226    1845 
   Size:        524     534     168     619

  Fragments arranged by size: 

                 619     534     524     168
 
PmlI CAC'GTG

Cuts at:      0    1314    1845 
   Size:       1314     531
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741    1200    1232    1845 
   Size:        709      32     459      32     613

  Fragments arranged by size: 

                 709     613     459      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1230    1552    1845 
   Size:       1230     322     293

  Fragments arranged by size: 

                1230     322     293
 
PspOMI G'GGCC_C

Cuts at:      0     410     574    1458    1845 
   Size:        410     164     884     387

  Fragments arranged by size: 

                 884     410     387     164
 
PspXI vC'TCGA_Gb

Cuts at:      0    1728    1845 
   Size:       1728     117
 
PstI C_TGCA'G

Cuts at:      0     788     794    1031    1845 
   Size:        788       6     237     814

  Fragments arranged by size: 

                 814     788     237       6
 
PvuII CAG'CTG

Cuts at:      0     595     789    1845 
   Size:        595     194    1056

  Fragments arranged by size: 

                1056     595     194
 
SacI G_AGCT'C

Cuts at:      0     182    1845 
   Size:        182    1663
 
SalI G'TCGA_C

Cuts at:      0     301    1845 
   Size:        301    1544
 
SbfI CC_TGCA'GG

Cuts at:      0    1031    1845 
   Size:       1031     814
 
SexAI A'CCwGG_T

Cuts at:      0      98    1845 
   Size:         98    1747
 
SfcI C'TryA_G

Cuts at:      0     784     790    1027    1845 
   Size:        784       6     237     818

  Fragments arranged by size: 

                 818     784     237       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     511    1405    1845 
   Size:        511     894     440

  Fragments arranged by size: 

                 894     511     440
 
SfoI GGC'GCC

Cuts at:      0     297    1845 
   Size:        297    1548
 
SmaI CCC'GGG

Cuts at:      0    1441    1845 
   Size:       1441     404
 
SmlI C'TyrA_G

Cuts at:      0     320    1728    1822    1845 
   Size:        320    1408      94      23

  Fragments arranged by size: 

                1408     320      94      23
 
StuI AGG'CCT

Cuts at:      0    1033    1841    1845 
   Size:       1033     808       4

  Fragments arranged by size: 

                1033     808       4
 
StyI C'CwwG_G

Cuts at:      0     113     845    1212    1344    1845 
   Size:        113     732     367     132     501

  Fragments arranged by size: 

                 732     501     367     132     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1219    1845 
   Size:       1219     626
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     216     248     406     438    1222    1254    1845 
   Size:        216      32     158      32     784      32     591

  Fragments arranged by size: 

                 784     591     216     158      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     870    1845 
   Size:        870     975
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      51     345    1845 
   Size:         51     294    1500

  Fragments arranged by size: 

                1500     294      51
 
XhoI C'TCGA_G

Cuts at:      0     320    1728    1845 
   Size:        320    1408     117

  Fragments arranged by size: 

                1408     320     117
 
XmaI C'CCGG_G

Cuts at:      0    1439    1845 
   Size:       1439     406
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AloI
    AlwNI     ApaI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI     SalI     SbfI    SexAI     SfcI     SfiI     SfoI     SmaI
     SmlI     StuI     StyI    TaqII     TstI     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AlfI    ApaLI     ApoI
     AscI     AseI    AsiSI     BaeI    BamHI     BcgI    BciVI     BclI
    BfrBI     BmtI     BplI    BsaBI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrGI   BssHII    BssSI    BstBI  BstZ17I     ClaI     DraI
   DraIII     DrdI    EcoRI    EcoRV  HindIII     HpaI     MfeI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII    SanDI     SapI     ScaI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XmnI     ZraI
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