Restriction map for pF1KB3641
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglII       BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DpnII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MroI        MspI        
MunI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         636  CGCG                 CG^CG
AciI          741  GCGG                 CCGC(-3/-1)
AfaI          540  GTAC                 GT^AC
AvrII          30  CCTAGG               C^CTAGG
BalI          119  TGGCCA               TGG^CCA
BbvI           90  GCAGC                GCAGC(8/12)
BglI           26  GCCTCCTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           30  CCTAGG               C^CTAGG
BpmI          440  CTCCAG               CTGGAG(16/14)
BsaI          214  GAGACC               GGTCTC(1/5)
BseRI         586  GAGGAG               GAGGAG(10/8)
BsiYI         657  CCGTGGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          657  CCGTGGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI         909  TCATGA               T^CATGA
BspMI         416  ACCTGC               ACCTGC(4/8)
BsrDI         761  GCAATG               GCAATG(2/0)
BstUI         636  CGCG                 CG^CG
Cac8I         420  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI          637  GCGC                 GCG^C
DraI          205  TTTAAA               TTT^AAA
DraII         626  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      626  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         814  GAATTC               G^AATTC
EcoT14I        30  CCTAGG               C^C[AT][AT]GG
Fnu4HI         90  GCAGC                GC^[ACGT]GC
HhaI          637  GCGC                 GCG^C
HinP1I        637  GCGC                 G^CGC
ItaI           90  GCAGC                GC^[ACGT]GC
MaeIII        397  GTCAC                ^GT[ACGT]AC
MluNI         119  TGGCCA               TGG^CCA
MscI          119  TGGCCA               TGG^CCA
MspA1I        685  CAGCTG               C[AC]G^C[GT]G
MvnI          636  CGCG                 CG^CG
NdeI          464  CATATG               CA^TATG
PpuMI         626  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         497  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         685  CAGCTG               CAG^CTG
RcaI          909  TCATGA               T^CATGA
RsaI          540  GTAC                 GT^AC
SfcI          551  CTACAG               C^T[GA][CT]AG
StyI           30  CCTAGG               C^C[AT][AT]GG
TaqI          313  TCGA                 T^CGA
TseI           90  GCAGC                G^C[AT]GC
Tsp45I        397  GTCAC                ^GT[GC]AC
TthHB8I       313  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          457  GTAGAC               GT^[AC][GT]AC
              859  GTAGAC              
AlwNI         110  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              625  CAGGACCTG           
AvaII         414  GGACC                G^G[AT]CC
              627  GGACC               
BfaI           31  CTAG                 C^TAG
              163  CTAG                
BsmAI         214  GAGAC                GTCTC(1/5)
              366  GTCTC               
BsmFI         650  GGGAC                GGGAC(10/14)
              448  GTCCC               
BsmI          174  GAATGC               GAATGC(1/-1)
              768  GCATTC              
BssKI         297  CCAGG                ^CC[ACGT]GG
              624  CCAGG               
BstNI         297  CCAGG                CC^[AT]GG
              624  CCAGG               
Cfr13I        414  GGACC                G^G[ACGT]CC
              627  GGACC               
DsaI          410  CCACGG               C^C[GA][CT]GG
              657  CCGTGG              
EaeI          119  TGGCCA               [CT]^GGCC[GA]
              471  TGGCCG              
EcoRII        297  CCAGG                ^CC[AT]GG
              624  CCAGG               
MaeI           31  CTAG                 C^TAG
              163  CTAG                
MseI           56  TTAA                 T^TAA
              206  TTAA                
MvaI          297  CCAGG                CC^[AT]GG
              624  CCAGG               
MwoI           26  GCCTCCTAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              601  GCATACAAGGC         
Sau96I        414  GGACC                G^G[ACGT]CC
              627  GGACC               
ScrFI         297  CCAGG                CC^[ACGT]GG
              624  CCAGG               
Tru9I          56  TTAA                 T^TAA
              206  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AcsI        AluI        ApoI        BsaJI       BsrI        
BstF5I      DdeI        FokI        HaeIII      HinfI       
HphI        MboII       MnlI        MslI        NlaIII      
PleI        SfaNI       TfiI        Tsp509I     TspRI       
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