Restriction map for
pF1KB3029
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AlwI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BamHI BciVI BclI BfrI BlnI
BsaBI BsaI BsaWI BseAI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DrdI
EcoO65I EcoRV FbaI FseI HindIII
HpaI KpnI MamI MluI MroI
MseI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SfiI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1788 GTCTAC GT^[AC][GT]AC
AhdI 1060 GACACCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 72 GTGCAC G^TGCAC
AlwNI 250 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 621 CCTGAGG CC^T[ACGT]AGG
Aor51HI 693 AGCGCT AGC^GCT
ApaI 1894 GGGCCC GGGCC^C
ApaLI 72 GTGCAC G^TGCAC
AspEI 1060 GACACCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 570 TGCGCA TGC^GCA
BbsI 802 GAAGAC GAAGAC(2/6)
BcgI 32 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 994 CTAG C^TAG
BglII 1598 AGATCT A^GATCT
BlpI 1855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1855 GCTGAGC GC^T[ACGT]AGC
BpuAI 802 GAAGAC GAAGAC(2/6)
BsgI 121 GTGCAG GTGCAG(16/14)
BsiWI 421 CGTACG C^GTACG
BsmAI 1328 GAGAC GTCTC(1/5)
BsmBI 1328 GAGACG CGTCTC(1/5)
BspHI 839 TCATGA T^CATGA
BsrBI 1890 GAGCGG CCGCTC(-3/-3)
BssHII 55 GCGCGC G^CGCGC
BssSI 601 CACGAG CACGAG(-5/-1)
BstYI 1598 AGATCT [GA]^GATC[CT]
Bsu36I 621 CCTGAGG CC^T[ACGT]AGG
CelII 1855 GCTGAGC GC^T[ACGT]AGC
DraIII 601 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1060 GACACCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 783 GAAGAG CTCTTC(1/4)
Eco47III 693 AGCGCT AGC^GCT
Eco81I 621 CCTGAGG CC^T[ACGT]AGG
EcoRI 583 GAATTC G^AATTC
FspI 570 TGCGCA TGC^GCA
HincII 822 GTTGAC GT[CT]^[GA]AC
HindII 822 GTTGAC GT[CT]^[GA]AC
Ksp632I 783 GAAGAG CTCTTC(1/4)
MaeI 994 CTAG C^TAG
MfeI 980 CAATTG C^AATTG
MflI 1598 AGATCT [GA]^GATC[CT]
MunI 980 CAATTG C^AATTG
NspI 440 ACATGC [GA]CATG^[CT]
PflMI 279 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1225 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 839 TCATGA T^CATGA
SexAI 410 ACCTGGT A^CC[AT]GGT
SgrAI 1644 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1777 AGGCCT AGG^CCT
TfiI 225 GAATC G^A[AT]TC
Van91I 279 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1016 CCAAGGGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1598 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 583 GAATTC [GA]^AATT[CT]
796 GAATTT
ApoI 583 GAATTC [GA]^AATT[CT]
796 GAATTT
BalI 1028 TGGCCA TGG^CCA
1811 TGGCCA
BbeI 269 GGCGCC GGCGC^C
668 GGCGCC
BbrPI 220 CACGTG CAC^GTG
388 CACGTG
BbvCI 453 CCTCAGC CCTCAGC(-5/-2)
1635 CCTCAGC
BsiEI 155 CGGCCG CG[GA][CT]^CG
372 CGGCCG
BsmI 713 GAATGC GAATGC(1/-1)
1285 GAATGC
BstAPI 74 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
249 GCAGACCCTGC
EagI 155 CGGCCG C^GGCCG
372 CGGCCG
EclXI 155 CGGCCG C^GGCCG
372 CGGCCG
Eco52I 155 CGGCCG C^GGCCG
372 CGGCCG
EcoNI 462 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
852 CCTGGAGGAGG
EcoT14I 1016 CCAAGG C^C[AT][AT]GG
1155 CCTTGG
EcoT22I 715 ATGCAT ATGCA^T
1287 ATGCAT
KasI 269 GGCGCC G^GCGCC
668 GGCGCC
MluNI 1028 TGGCCA TGG^CCA
1811 TGGCCA
MscI 1028 TGGCCA TGG^CCA
1811 TGGCCA
MslI 105 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
747 CATCAGTGTG
NarI 269 GGCGCC GG^CGCC
668 GGCGCC
NsiI 715 ATGCAT ATGCA^T
1287 ATGCAT
PmaCI 220 CACGTG CAC^GTG
388 CACGTG
PmlI 220 CACGTG CAC^GTG
388 CACGTG
SfoI 269 GGCGCC GGC^GCC
668 GGCGCC
StyI 1016 CCAAGG C^C[AT][AT]GG
1155 CCTTGG
Tsp45I 1710 GTCAC ^GT[GC]AC
1753 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp509I TspRI TthHB8I