Restriction map for
pF1KB3029
(Circular) (Six-base) MAPSORT of: pF1KB3029.seq Check: 8633 from: 1 to: 5144
pF1KB3029 5144 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 692 692
Size: 5144
AccI GT'mk_AC
Cuts at: 1897 2182 1897
Size: 285 4859
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 1415 2969 3401 3754 4416 816
Size: 599 1554 432 353 662 1544
Fragments arranged by size:
1554 1544 662 599 432 353
AfeI AGC'GCT
Cuts at: 803 803
Size: 5144
AflIII A'CryG_T
Cuts at: 2496 4296 4577 2496
Size: 1800 281 3063
Fragments arranged by size:
3063 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1173 1173
Size: 5144
AlwNI CAG_nnn'CTG
Cuts at: 363 2449 3887 363
Size: 2086 1438 1620
Fragments arranged by size:
2086 1620 1438
ApaI G_GGCC'C
Cuts at: 2006 2006
Size: 5144
ApaLI G'TGCA_C
Cuts at: 180 3982 180
Size: 3802 1342
ApoI r'AATT_y
Cuts at: 691 904 2148 4497 4753 691
Size: 213 1244 2349 256 1082
Fragments arranged by size:
2349 1244 1082 256 213
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 783 1303 1789 2164 783
Size: 520 486 375 3763
Fragments arranged by size:
3763 520 486 375
BamHI G'GATC_C
Cuts at: 2169 2169
Size: 5144
BanI G'GyrC_C
Cuts at: 377 467 776 1270 2160 2574 2822 2857 377
Size: 90 309 494 890 414 248 35 2664
Fragments arranged by size:
2664 890 494 414 309 248 90 35
BanII G_rGCy'C
Cuts at: 629 1262 1601 2006 2069 2158 3188 629
Size: 633 339 405 63 89 1030 2585
Fragments arranged by size:
2585 1030 633 405 339 89 63
BbeI G_GCGC'C
Cuts at: 381 780 2578 2826 381
Size: 399 1798 248 2699
Fragments arranged by size:
2699 1798 399 248
BbsI GAAGACnn'nnnn_
Cuts at: 917 917
Size: 5144
BbvCI CC'TCA_GC
Cuts at: 562 1744 562
Size: 1182 3962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 129 163 129
Size: 34 5110
BciVI GTATCCnnnnn_n'
Cuts at: 3042 4098 5144 3042
Size: 1056 1046 3042
Fragments arranged by size:
3042 1056 1046
BclI T'GATC_A
Cuts at: 2664 2664
Size: 5144
BfrBI ATG'CAT
Cuts at: 825 1397 825
Size: 572 4572
BglI GCCn_nnn'nGGC
Cuts at: 785 1144 1675 1974 785
Size: 359 531 299 3955
Fragments arranged by size:
3955 531 359 299
BglII A'GATC_T
Cuts at: 1706 2659 4696 1706
Size: 953 2037 2154
Fragments arranged by size:
2154 2037 953
BlpI GC'TnA_GC
Cuts at: 1964 2256 1964
Size: 292 4852
Bme1580I G_kGCm'C
Cuts at: 184 541 1275 2006 2082 2769 2862 3986 184
Size: 357 734 731 76 687 93 1124 1342
Fragments arranged by size:
1342 1124 734 731 687 357 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 2356 2437 2772 4916 2356
Size: 81 335 2144 2584
Fragments arranged by size:
2584 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 820 3511 820
Size: 2691 2453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 559 982 1069 1492 1695 1714 223
Size: 336 423 87 423 203 19 3653
Fragments arranged by size:
3653 423 423 336 203 87 19
Bpu10I CC'TnA_GC
Cuts at: 562 1744 562
Size: 1182 3962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 3199 3913 4211 2320
Size: 879 714 298 3253
Fragments arranged by size:
3253 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4848 4848
Size: 5144
BsaAI yAC'GTr
Cuts at: 330 498 591 2497 330
Size: 168 93 1906 2977
Fragments arranged by size:
2977 1906 168 93
BsaBI GATnn'nnATC
Cuts at: 6 2658 4695 6
Size: 2652 2037 455
Fragments arranged by size:
2652 2037 455
BsaHI Gr'CG_yC
Cuts at: 378 777 1831 2575 2823 3525 5021 378
Size: 399 1054 744 248 702 1496 501
Fragments arranged by size:
1496 1054 744 702 501 399 248
BsaWI w'CCGG_w
Cuts at: 2333 2854 3532 3943 4090 4364 2333
Size: 521 678 411 147 274 3113
Fragments arranged by size:
3113 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 955 985 955
Size: 30 5114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 979 982 1066 1207 1225 1392 2041 2053
Size: 730 3 84 141 18 167 649 12
Cuts at: 2053 2056 2068 249
Size: 3 12 3325
Fragments arranged by size:
3325 730 649 167 141 84 18 12
12 3 3
BseYI C'CCAG_C
Cuts at: 994 2569 3327 3992 4400 994
Size: 1575 758 665 408 1738
Fragments arranged by size:
1738 1575 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 250
Size: 5144
BsiEI CG_ry'CG
Cuts at: 104 266 483 2686 2732 3962 104
Size: 162 217 2203 46 1230 1286
Fragments arranged by size:
2203 1286 1230 217 162 46
BsiHKAI G_wGCw'C
Cuts at: 184 629 1262 1601 1736 2158 2936 3126 3986
Size: 445 633 339 135 422 778 190 860
Cuts at: 3986 184
Size: 1342
Fragments arranged by size:
1342 860 778 633 445 422 339 190
135
BsiWI C'GTAC_G
Cuts at: 529 529
Size: 5144
BsmI GAATG_Cn'
Cuts at: 827 1399 827
Size: 572 4572
BsmBI CGTCTCn'nnnn_
Cuts at: 1430 1430
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 184 541 629 1262 1275 1601 1736 2006 2069
Size: 357 88 633 13 326 135 270 63
Cuts at: 2069 2082 2158 2769 2862 2936 3126 3188 3986
Size: 13 76 611 93 74 190 62 798
Cuts at: 3986 184
Size: 1342
Fragments arranged by size:
1342 798 633 611 357 326 270 190
135 93 88 76 74 63 62 13
13
BspHI T'CATG_A
Cuts at: 947 3576 947
Size: 2629 2515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 692 1559 2194 2408 2710 3091 578
Size: 114 867 635 214 302 381 2631
Fragments arranged by size:
2631 867 635 381 302 214 114
BsrBI CCG'CTC
Cuts at: 50 2000 3436 3490 4974 5139 50
Size: 1950 1436 54 1484 165 55
Fragments arranged by size:
1950 1484 1436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3056 3056
Size: 5144
BsrFI r'CCGG_y
Cuts at: 642 773 1112 1753 1833 3142 3323 642
Size: 131 339 641 80 1309 181 2463
Fragments arranged by size:
2463 1309 641 339 181 131 80
BssHII G'CGCG_C
Cuts at: 163 163
Size: 5144
BssSI C'ACGA_G
Cuts at: 709 3415 4123 709
Size: 2706 708 1730
Fragments arranged by size:
2706 1730 708
BstAPI GCAn_nnn'nTGC
Cuts at: 188 363 2521 188
Size: 175 2158 2811
Fragments arranged by size:
2811 2158 175
BstBI TT'CG_AA
Cuts at: 3505 3505
Size: 5144
BstYI r'GATC_y
Cuts at: 1 1706 2169 2636 2659 2994 3240 3546 3558
Size: 1705 463 467 23 335 246 306 12
Cuts at: 3558 3644 4696 1
Size: 86 1052 449
Fragments arranged by size:
1705 1052 467 463 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 730 730
Size: 5144
BtgI C'CryG_G
Cuts at: 126 266 477 1223 1922 126
Size: 140 211 746 699 3348
Fragments arranged by size:
3348 746 699 211 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2135 3094 3275 3438 2135
Size: 959 181 163 3841
Fragments arranged by size:
3841 959 181 163
BtsI GCAGTG_nn'
Cuts at: 420 1355 2387 420
Size: 935 1032 3177
Fragments arranged by size:
3177 1032 935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1898 1933 1898
Size: 35 5109
DraI TTT'AAA
Cuts at: 2143 2143
Size: 5144
DraIII CAC_nnn'GTG
Cuts at: 714 714
Size: 5144
DrdI GACnn_nn'nnGTC
Cuts at: 2850 4194 2850
Size: 1344 3800
EaeI y'GGCC_r
Cuts at: 263 455 480 644 1114 1136 1226 1835 1919
Size: 192 25 164 470 22 90 609 84
Cuts at: 1919 2729 2903 3294 3321 4666 263
Size: 810 174 391 27 1345 741
Fragments arranged by size:
1345 810 741 609 470 391 192 174
164 90 84 27 25 22
EagI C'GGCC_G
Cuts at: 263 480 2729 263
Size: 217 2249 2678
Fragments arranged by size:
2678 2249 217
EarI CTCTTCn'nnn_
Cuts at: 886 3167 3377 886
Size: 2281 210 2653
Fragments arranged by size:
2653 2281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3352 4094 4240 4954 3352
Size: 742 146 714 3542
Fragments arranged by size:
3542 742 714 146
EcoICRI GAG'CTC
Cuts at: 627 1260 1599 2156 627
Size: 633 339 557 3615
Fragments arranged by size:
3615 633 557 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 559 816 982 1069 1415 1492 1695 1714
Size: 336 257 166 87 346 77 203 19
Cuts at: 1714 2969 3401 3754 4416 223
Size: 1255 432 353 662 951
Fragments arranged by size:
1255 951 662 432 353 346 336 257
203 166 87 77 19
EcoNI CCTnn'n_nnAGG
Cuts at: 574 964 574
Size: 390 4754
EcoO109I rG'GnC_Cy
Cuts at: 1131 1334 1740 1976 2283 1131
Size: 203 406 236 307 3992
Fragments arranged by size:
3992 406 307 236 203
EcoRI G'AATT_C
Cuts at: 691 2148 691
Size: 1457 3687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 5144
FspI TGC'GCA
Cuts at: 680 2925 680
Size: 2245 2899
HaeII r_GCGC'y
Cuts at: 381 780 805 1754 1802 2578 2826 4056 4827
Size: 399 25 949 48 776 248 1230 771
Cuts at: 4827 381
Size: 698
Fragments arranged by size:
1230 949 776 771 698 399 248 48
25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 967 999 1159 1191 2986 3016 3018 3048 967
Size: 32 160 32 1795 30 2 30 3063
Fragments arranged by size:
3063 1795 160 32 32 30 30 2
HincII GTy'rAC
Cuts at: 932 2183 932
Size: 1251 3893
KasI G'GCGC_C
Cuts at: 377 776 2574 2822 377
Size: 399 1798 248 2699
Fragments arranged by size:
2699 1798 399 248
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MfeI C'AATT_G
Cuts at: 1088 1088
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1481 3903 4087 1481
Size: 2422 184 2538
Fragments arranged by size:
2538 2422 184
MscI TGG'CCA
Cuts at: 1138 1921 2905 1138
Size: 783 984 3377
Fragments arranged by size:
3377 984 783
MslI CAynn'nnrTG
Cuts at: 217 859 3133 3260 217
Size: 642 2274 127 2101
Fragments arranged by size:
2274 2101 642 127
MspA1I CmG'CkG
Cuts at: 128 248 405 411 479 994 1225 2255 2569
Size: 120 157 6 68 515 231 1030 314
Cuts at: 2569 2929 3713 3958 128
Size: 360 784 245 1314
Fragments arranged by size:
1314 1030 784 515 360 314 245 231
157 120 68 6
NaeI GCC'GGC
Cuts at: 644 775 1114 1755 1835 3325 644
Size: 131 339 641 80 1490 2463
Fragments arranged by size:
2463 1490 641 339 131 80
NarI GG'CG_CC
Cuts at: 378 777 2575 2823 378
Size: 399 1798 248 2699
Fragments arranged by size:
2699 1798 399 248
NgoMIV G'CCGG_C
Cuts at: 642 773 1112 1753 1833 3323 642
Size: 131 339 641 80 1490 2463
Fragments arranged by size:
2463 1490 641 339 131 80
NsiI A_TGCA'T
Cuts at: 827 1399 827
Size: 572 4572
NspI r_CATG'y
Cuts at: 552 2197 2697 4300 552
Size: 1645 500 1603 1396
Fragments arranged by size:
1645 1603 1396 500
PasI CC'CwG_GG
Cuts at: 1329 1329
Size: 5144
PciI A'CATG_T
Cuts at: 4296 4296
Size: 5144
PflMI CCAn_nnn'nTGG
Cuts at: 393 393
Size: 5144
PfoI T'CCnGG_A
Cuts at: 554 554
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PmlI CAC'GTG
Cuts at: 330 498 330
Size: 168 4976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 217 249 3590 3622 217
Size: 32 3341 32 1739
Fragments arranged by size:
3341 1739 32 32
PpuMI rG'GwC_Cy
Cuts at: 1334 1334
Size: 5144
PspOMI G'GGCC_C
Cuts at: 2002 2002
Size: 5144
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1531 1563 1531
Size: 32 5112
PstI C_TGCA'G
Cuts at: 368 410 1362 2087 2191 2876 368
Size: 42 952 725 104 685 2636
Fragments arranged by size:
2636 952 725 685 104 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 405 411 994 2569 2929 405
Size: 6 583 1575 360 2620
Fragments arranged by size:
2620 1575 583 360 6
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 629 1262 1601 2158 629
Size: 633 339 557 3615
Fragments arranged by size:
3615 633 557 339
SacII CC_GC'GG
Cuts at: 129 480 1226 129
Size: 351 746 4047
Fragments arranged by size:
4047 746 351
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SanDI GG'GwC_CC
Cuts at: 1334 1334
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 3167 3377 3167
Size: 210 4934
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SexAI A'CCwGG_T
Cuts at: 518 518
Size: 5144
SfcI C'TryA_G
Cuts at: 33 364 406 739 1358 2083 2187 2872 3840
Size: 331 42 333 619 725 104 685 968
Cuts at: 3840 4031 33
Size: 191 1146
Fragments arranged by size:
1146 968 725 685 619 333 331 191
104 42
SfoI GGC'GCC
Cuts at: 379 778 2576 2824 379
Size: 399 1798 248 2699
Fragments arranged by size:
2699 1798 399 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SgrAI Cr'CCGG_yG
Cuts at: 1753 1753
Size: 5144
SmaI CCC'GGG
Cuts at: 2166 2166
Size: 5144
SmlI C'TyrA_G
Cuts at: 2299 3214 3928 4190 2299
Size: 915 714 262 3253
Fragments arranged by size:
3253 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5144
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
StuI AGG'CCT
Cuts at: 1887 1887
Size: 5144
StyI C'CwwG_G
Cuts at: 1124 1263 2278 2632 1124
Size: 139 1015 354 3636
Fragments arranged by size:
3636 1015 354 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 2727 2860 3319 3528 304
Size: 2423 133 459 209 1920
Fragments arranged by size:
2423 1920 459 209 133
TatI w'GTAC_w
Cuts at: 4659 4659
Size: 5144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 700 732 955 987 4661 4693 163
Size: 32 505 32 223 32 3674 32 614
Fragments arranged by size:
3674 614 505 223 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1131 2639 1131
Size: 1508 3636
XmaI C'CCGG_G
Cuts at: 2164 2164
Size: 5144
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AleI AlfI AloI AscI AvrII
BaeI BmgBI BmtI BspEI BsrGI BstEII BstXI BstZ17I
ClaI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI PacI PshAI PsiI PspXI SfiI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI