Restriction map for
pF1KA0308
##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AspEI
AspI AviII BbeI BbrPI BcgI
BciVI BglI BlpI Bpu1102I BsgI
BsiEI BsrBI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII CfoI
CpoI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MluI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2325 GGTACC G^GTACC
AccI 1249 GTCTAC GT^[AC][GT]AC
AccII 4005 CGCG CG^CG
AccIII 1781 TCCGGA T^CCGGA
AciI 5232 GCGG CCGC(-3/-1)
AclI 142 AACGTT AA^CGTT
AcyI 2699 GGCGTC G[GA]^CG[CT]C
AseI 4813 ATTAAT AT^TAAT
AsnI 4813 ATTAAT AT^TAAT
Asp718I 2325 GGTACC G^GTACC
AvrII 3436 CCTAGG C^CTAGG
BalI 4117 TGGCCA TGG^CCA
BamHI 3063 GGATCC G^GATCC
BbvCI 799 GCTGAGG CCTCAGC(-5/-2)
BlnI 3436 CCTAGG C^CTAGG
BsaAI 126 TACGTG [CT]AC^GT[GA]
BsaHI 2699 GGCGTC G[GA]^CG[CT]C
BseAI 1781 TCCGGA T^CCGGA
BsiWI 124 CGTACG C^GTACG
Bsp1407I 5823 TGTACA T^GTACA
BspDI 1477 ATCGAT AT^CGAT
BspEI 1781 TCCGGA T^CCGGA
BspLU11I 1156 ACATGT A^CATGT
BsrDI 868 GCAATG GCAATG(2/0)
BsrGI 5823 TGTACA T^GTACA
BssSI 2036 CACGAG CACGAG(-5/-1)
BstUI 4005 CGCG CG^CG
ClaI 1477 ATCGAT AT^CGAT
DrdI 4062 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4781 GAATTC G^AATTC
EcoRV 1474 GATATC GAT^ATC
FauI 5232 GCGGG CCCGC(4/6)
Hin1I 2699 GGCGTC G[GA]^CG[CT]C
HindIII 3187 AAGCTT A^AGCTT
KpnI 2325 GGTACC GGTAC^C
MluNI 4117 TGGCCA TGG^CCA
MroI 1781 TCCGGA T^CCGGA
MscI 4117 TGGCCA TGG^CCA
MvnI 4005 CGCG CG^CG
PpuMI 66 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1529 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4813 ATTAAT AT^TAAT
Psp1406I 142 AACGTT AA^CGTT
ScaI 3967 AGTACT AGT^ACT
SmaI 4893 CCCGGG CCC^GGG
SspBI 5823 TGTACA T^GTACA
XbaI 3465 TCTAGA T^CTAGA
XmaCI 4893 CCCGGG C^CCGGG
XmaI 4893 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 477 CTTAAG C^TTAAG
4882 CTTAAG
BclI 4136 TGATCA T^GATCA
4326 TGATCA
BfrI 477 CTTAAG C^TTAAG
4882 CTTAAG
BglII 1401 AGATCT A^GATCT
2610 AGATCT
BsaWI 1574 ACCGGA [AT]^CCGG[AT]
1781 TCCGGA
BspMI 1844 ACCTGC ACCTGC(4/8)
2556 ACCTGC
BsrFI 4306 GCCGGT [GA]^CCGG[CT]
5431 ACCGGC
BstBI 1409 TTCGAA TT^CGAA
2147 TTCGAA
Cfr10I 4306 GCCGGT [GA]^CCGG[CT]
5431 ACCGGC
DraI 2404 TTTAAA TTT^AAA
2572 TTTAAA
DraIII 400 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3271 CACGGGGTG
EaeI 1890 TGGCCG [CT]^GGCC[GA]
4117 TGGCCA
FbaI 4136 TGATCA T^GATCA
4326 TGATCA
MfeI 305 CAATTG C^AATTG
1514 CAATTG
MslI 2410 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3012 CATGTCCATG
MunI 305 CAATTG C^AATTG
1514 CAATTG
NdeI 1274 CATATG CA^TATG
1998 CATATG
NspV 1409 TTCGAA TT^CGAA
2147 TTCGAA
PstI 1145 CTGCAG CTGCA^G
5699 CTGCAG
SacI 757 GAGCTC GAGCT^C
1940 GAGCTC
SfuI 1409 TTCGAA TT^CGAA
2147 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI