Restriction map for pF1KB7575
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# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FbaI        Fnu4HI      
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        KspI        MaeI        MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TseI        Tsp45I      Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          443  GTATAC               GT^[AC][GT]AC
AccII          72  CGCG                 CG^CG
AciI           71  CCGC                 CCGC(-3/-1)
AfaI          581  GTAC                 GT^AC
AlwI          172  GATCC                GGATC(4/5)
BbvCI          93  CCTCAGC              CCTCAGC(-5/-2)
BcgI           82  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI           26  CTGGAG               CTGGAG(16/14)
BsiYI         217  CCAACAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          217  CCAACAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI         374  TCATGA               T^CATGA
BsrDI          33  GCAATG               GCAATG(2/0)
BssKI          21  CCAGG                ^CC[ACGT]GG
Bst1107I      443  GTATAC               GTA^TAC
BstAPI         57  GCAACATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        144  GGATG                GGATG(2/0)
BstNI          21  CCAGG                CC^[AT]GG
BstUI          72  CGCG                 CG^CG
BstZ17I       443  GTATAC               GTA^TAC
DdeI           94  CTCAG                C^T[ACGT]AG
DraI          642  TTTAAA               TTT^AAA
EarI          268  GAAGAG               CTCTTC(1/4)
EcoRII         21  CCAGG                ^CC[AT]GG
EcoT14I        99  CCTTGG               C^C[AT][AT]GG
FauI           70  CCCGC                CCCGC(4/6)
FokI          144  GGATG                GGATG(9/13)
HphI          321  TCACC                GGTGA(8/7)
Ksp632I       268  GAAGAG               CTCTTC(1/4)
MaeII         606  ACGT                 A^CGT
MslI          280  CACAGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           21  CCAGG                CC^[AT]GG
MvnI           72  CGCG                 CG^CG
PflMI         217  CCAACAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          240  GAGTC                GAGTC(4/5)
RcaI          374  TCATGA               T^CATGA
RsaI          581  GTAC                 GT^AC
SapI          268  GAAGAGC              GCTCTTC(1/4)
ScrFI          21  CCAGG                CC^[ACGT]GG
StyI           99  CCTTGG               C^C[AT][AT]GG
Van91I        217  CCAACAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI          470  AGCT                 AG^CT
              619  AGCT                
BsaJI          20  CCCAGG               C^C[ACGT][ACGT]GG
               99  CCTTGG              
BsrI          223  ACTGG                ACTGG(1/-1)
              326  CCAGT               
DpnII         131  GATC                 ^GATC
              172  GATC                
EcoRI         370  GAATTC               G^AATTC
              625  GAATTC              
MboI          131  GATC                 ^GATC
              172  GATC                
MwoI           10  GCTCCCGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               57  GCAACATCTGC         
NdeII         131  GATC                 ^GATC
              172  GATC                
NlaIII        375  CATG                 CATG^
              417  CATG                
Sau3AI        131  GATC                 ^GATC
              172  GATC                
SfaNI         499  GCATC                GCATC(5/9)
              601  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AcsI        ApoI        HinfI       MboII       MnlI        
MseI        TfiI        Tru9I       Tsp509I     TspRI       
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