Restriction map for
pF1KB7575
(Circular) (Six-base) MAPSORT of: pF1KB7575.seq Check: 7586 from: 1 to: 3761
pF1KB7575 3761 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 552 799 552
Size: 247 3514
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 755 3601 755
Size: 2846 915
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 395 1586 2018 2765 3033 265
Size: 130 1191 432 747 268 993
Fragments arranged by size:
1191 993 747 432 268 130
AflIII A'CryG_T
Cuts at: 1113 2217 3194 1113
Size: 1104 977 1680
Fragments arranged by size:
1680 1104 977
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 270 478 540 733 765 3114 3370 270
Size: 208 62 193 32 2349 256 661
Fragments arranged by size:
2349 661 256 208 193 62 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 781 781
Size: 3761
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 777 1191 1439 1474 777
Size: 414 248 35 3064
Fragments arranged by size:
3064 414 248 35
BanII G_rGCy'C
Cuts at: 775 1805 775
Size: 1030 2731
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BbvCI CC'TCA_GC
Cuts at: 202 202
Size: 3761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 179 213 90
Size: 34 55 34 3638
Fragments arranged by size:
3638 55 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglII A'GATC_T
Cuts at: 1276 3313 1276
Size: 2037 1724
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 1386 1479 2535 1386
Size: 93 1056 2612
Fragments arranged by size:
2612 1056 93
BmgBI CAC'GTC
Cuts at: 715 715
Size: 3761
BmrI ACTGGGnnnn_n'
Cuts at: 428 973 1054 1389 3533 428
Size: 545 81 335 2144 656
Fragments arranged by size:
2144 656 545 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 3761
Bpu10I CC'TnA_GC
Cuts at: 202 202
Size: 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3465 3465
Size: 3761
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 306 336 306
Size: 30 3731
BseYI C'CCAG_C
Cuts at: 1186 1944 2521 3017 1186
Size: 758 577 496 1930
Fragments arranged by size:
1930 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1303 1349 2557 104
Size: 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 775 1553 1743 2535 775
Size: 778 190 792 2001
Fragments arranged by size:
2001 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 775 1386 1479 1553 1743 1805 2535 775
Size: 611 93 74 190 62 730 2001
Fragments arranged by size:
2001 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 482 2937 482
Size: 2455 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 811 1025 1327 1708 811
Size: 214 302 381 2864
Fragments arranged by size:
2864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2053 2107 3591 3756 50
Size: 2003 54 1484 165 55
Fragments arranged by size:
2003 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 148 1673 148
Size: 1525 2236
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BssSI C'ACGA_G
Cuts at: 2032 2390 2032
Size: 358 3403
BstAPI GCAn_nnn'nTGC
Cuts at: 171 1138 171
Size: 967 2794
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 786 1253 1276 1611 1857 2869 2955 2967
Size: 785 467 23 335 246 1012 86 12
Cuts at: 2967 3313 1
Size: 346 449
Fragments arranged by size:
1012 785 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 553 553
Size: 3761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 160 1004 160
Size: 844 2917
DraI TTT'AAA
Cuts at: 752 760 752
Size: 8 3753
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 1346 1520 1911 1938 3283 1346
Size: 174 391 27 1345 1824
Fragments arranged by size:
1824 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 371 1784 1994 371
Size: 1413 210 2138
Fragments arranged by size:
2138 1413 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 265 395 1586 2018 2765 3033 155
Size: 110 130 1191 432 747 268 883
Fragments arranged by size:
1191 883 747 432 268 130 110
EcoO109I rG'GnC_Cy
Cuts at: 900 900
Size: 3761
EcoRI G'AATT_C
Cuts at: 478 733 765 478
Size: 255 32 3474
Fragments arranged by size:
3474 255 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3761
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 1195 1443 2465 3444 1195
Size: 248 1022 979 1512
Fragments arranged by size:
1512 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 496 528 1603 1633 1635 1665 496
Size: 32 1075 30 2 30 2592
Fragments arranged by size:
2592 1075 32 30 30 2
HincII GTy'rAC
Cuts at: 800 800
Size: 3761
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 349 2432 2616 230
Size: 119 2083 184 1375
Fragments arranged by size:
2083 1375 184 119
MscI TGG'CCA
Cuts at: 1522 1522
Size: 3761
MslI CAynn'nnrTG
Cuts at: 392 1750 1877 392
Size: 1358 127 2276
Fragments arranged by size:
2276 1358 127
MspA1I CmG'CkG
Cuts at: 872 1186 1546 2559 2804 872
Size: 314 360 1013 245 1829
Fragments arranged by size:
1829 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 814 1314 2221 814
Size: 500 907 2354
Fragments arranged by size:
2354 907 500
PciI A'CATG_T
Cuts at: 2217 2217
Size: 3761
PflMI CCAn_nnn'nTGG
Cuts at: 331 331
Size: 3761
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PsiI TTA'TAA
Cuts at: 313 313
Size: 3761
PstI C_TGCA'G
Cuts at: 808 1493 808
Size: 685 3076
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 1186 1546 1186
Size: 360 3401
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 371 1784 1994 371
Size: 1413 210 2138
Fragments arranged by size:
2138 1413 210
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 804 1489 2482 2673 33
Size: 771 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 916 1831 2323 2585 916
Size: 915 492 262 2092
Fragments arranged by size:
2092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
StyI C'CwwG_G
Cuts at: 207 895 1249 207
Size: 688 354 2719
Fragments arranged by size:
2719 688 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 1477 1936 2145 1344
Size: 133 459 209 2960
Fragments arranged by size:
2960 459 209 133
TatI w'GTAC_w
Cuts at: 688 3276 688
Size: 2588 1173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 291 323 3278 3310 291
Size: 32 2955 32 742
Fragments arranged by size:
2955 742 32 32
Tth111I GACn'n_nGTC
Cuts at: 1558 1558
Size: 3761
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BglI BmtI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI