Restriction map for pF1KE0200
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoRI       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RcaI        RsrII       SacI        SalI        
SapI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AclI           55  AACGTT               AA^CGTT
AcsI          199  AAATTC               [GA]^AATT[CT]
AcyI          174  GGCGCC               G[GA]^CG[CT]C
AfaI          573  GTAC                 GT^AC
ApoI          199  AAATTC               [GA]^AATT[CT]
Asp700I       195  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         760  GTGCTC               G[AT]GC[AT]^C
AvaI          748  CCCGAG               C^[CT]CG[GA]G
BbeI          174  GGCGCC               GGCGC^C
BbsI          625  GTCTTC               GAAGAC(2/6)
BfaI          233  CTAG                 C^TAG
BpuAI         625  GTCTTC               GAAGAC(2/6)
BsaHI         174  GGCGCC               G[GA]^CG[CT]C
BsaI          409  GAGACC               GGTCTC(1/5)
BsgI          100  GTGCAG               GTGCAG(16/14)
BsiHKAI       760  GTGCTC               G[AT]GC[AT]^C
BsmFI         228  GTCCC                GGGAC(10/14)
BsoBI         748  CCCGAG               C^[CT]CG[GA]G
BspDI         829  ATCGAT               AT^CGAT
BspMI         575  ACCTGC               ACCTGC(4/8)
BsrI          927  ACTGG                ACTGG(1/-1)
BssHII        508  GCGCGC               G^CGCGC
BstEII        348  GGTGACC              G^GT[ACGT]ACC
BstPI         348  GGTGACC              G^GT[ACGT]ACC
ClaI          829  ATCGAT               AT^CGAT
EarI         1291  GAAGAG               CTCTTC(1/4)
EcoO65I       348  GGTGACC              G^GT[ACGT]ACC
EcoRV         187  GATATC               GAT^ATC
EcoT14I      1217  CCAAGG               C^C[AT][AT]GG
HgaI          551  GCGTC                GACGC(5/10)
Hin1I         174  GGCGCC               G[GA]^CG[CT]C
KasI          174  GGCGCC               G^GCGCC
Ksp632I      1291  GAAGAG               CTCTTC(1/4)
KspI          540  CCGCGG               CCGC^GG
MaeI          233  CTAG                 C^TAG
MseI         1232  TTAA                 T^TAA
NarI          174  GGCGCC               GG^CGCC
PpuMI        1081  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I       55  AACGTT               AA^CGTT
PvuII         337  CAGCTG               CAG^CTG
RsaI          573  GTAC                 GT^AC
SacII         540  CCGCGG               CCGC^GG
SbfI          576  CCTGCAGG             CCTGCA^GG
SfoI          174  GGCGCC               GGC^GCC
SmlI          934  CTCAAG               C^T[CT][GA]AG
Sse8387I      576  CCTGCAGG             CCTGCA^GG
StyI         1217  CCAAGG               C^C[AT][AT]GG
TfiI          706  GATTC                G^A[AT]TC
Tru9I        1232  TTAA                 T^TAA
Tsp509I       200  AATT                 ^AATT
XmnI          195  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AspI          723  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1027  GACAGTGTC           
AvaII         930  GGTCC                G^G[AT]CC
             1082  GGTCC               
BanI          174  GGCGCC               G^G[CT][GA]CC
              941  GGTGCC              
BanII         247  GGGCTC               G[GA]GC[CT]^C
              751  GAGCCC              
BsiEI         329  CGGCCG               CG[GA][CT]^CG
              461  CGGCCG              
BsmBI         477  CGTCTC               CGTCTC(1/5)
              948  CGTCTC              
BsrBI         240  CCGCTC               CCGCTC(-3/-3)
              450  GAGCGG              
BstF5I        432  CATCC                GGATG(2/0)
             1251  CATCC               
BstYI         582  GGATCT               [GA]^GATC[CT]
             1184  AGATCC              
DraII        1081  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1211  AGGCCCC             
DsaI          326  CCACGG               C^C[GA][CT]GG
              540  CCGCGG              
EagI          329  CGGCCG               C^GGCCG
              461  CGGCCG              
EclXI         329  CGGCCG               C^GGCCG
              461  CGGCCG              
Eco52I        329  CGGCCG               C^GGCCG
              461  CGGCCG              
EcoO109I     1081  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1211  AGGCCCC             
FokI          432  CATCC                GGATG(9/13)
             1251  CATCC               
MflI          582  GGATCT               [GA]^GATC[CT]
             1184  AGATCC              
NlaIII        372  CATG                 CATG^
              766  CATG                
PflFI         723  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1027  GACAGTGTC           
PstI          163  CTGCAG               CTGCA^G
              577  CTGCAG              
SfaNI          45  GATGC                GCATC(5/9)
              608  GCATC               
SfcI          163  CTGCAG               C^T[GA][CT]AG
              577  CTGCAG              
TspRI         926  CACTG                CA[GC]TG[ACGT][ACGT]^
             1029  CAGTG               
Tth111I       723  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1027  GACAGTGTC           
XhoII         582  GGATCT               [GA]^GATC[CT]
             1184  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       TaqI        TseI        
Tsp45I      TthHB8I     
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