Restriction map for pF1KE0200
 (Linear) (Six-base) MAPSORT of: KIEE0200.seq  Check: 2893  from: 1  to: 1362



KIEE0200.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AclI AA'CG_TT

Cuts at:      0      56    1362 
   Size:         56    1306
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    1362 
   Size:       1310      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0     554     585     880    1362 
   Size:        554      31     295     482

  Fragments arranged by size: 

                 554     482     295      31
 
ApoI r'AATT_y

Cuts at:      0     199    1362 
   Size:        199    1163
 
AvaI C'yCGr_G

Cuts at:      0     748    1362 
   Size:        748     614
 
BanI G'GyrC_C

Cuts at:      0     174     941    1362 
   Size:        174     767     421

  Fragments arranged by size: 

                 767     421     174
 
BanII G_rGCy'C

Cuts at:      0     251     755    1362 
   Size:        251     504     607

  Fragments arranged by size: 

                 607     504     251
 
BbeI G_GCGC'C

Cuts at:      0     178    1362 
   Size:        178    1184
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     618    1362 
   Size:        618     744
 
BmgBI CAC'GTC

Cuts at:      0     948    1362 
   Size:        948     414
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     875    1362 
   Size:        875     487
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     549     666     917    1362 
   Size:        361     188     117     251     445

  Fragments arranged by size: 

                 445     361     251     188     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1362 
   Size:        919     443
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     403    1362 
   Size:        403     959
 
BsaHI Gr'CG_yC

Cuts at:      0     175    1362 
   Size:        175    1187
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     359     389    1362 
   Size:        359      30     973

  Fragments arranged by size: 

                 973     359      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     142     380     708     784     817     905    1111 
   Size:        139       3     238     328      76      33      88     206

Cuts at:   1111    1155    1285    1362 
   Size:         44     130      77

  Fragments arranged by size: 

                 328     238     206     139     130      88      77      76
                  44      33       3
 
BseYI C'CCAG_C

Cuts at:      0     178     360    1362 
   Size:        178     182    1002

  Fragments arranged by size: 

                1002     182     178
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1362 
   Size:        121    1241
 
BsiEI CG_ry'CG

Cuts at:      0     332     464    1362 
   Size:        332     132     898

  Fragments arranged by size: 

                 898     332     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     764    1362 
   Size:        764     598
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     483     954    1362 
   Size:        483     471     408

  Fragments arranged by size: 

                 483     471     408
 
Bsp1286I G_dGCh'C

Cuts at:      0     251     755     764    1362 
   Size:        251     504       9     598

  Fragments arranged by size: 

                 598     504     251       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     584    1362 
   Size:        584     778
 
BsrBI CCG'CTC

Cuts at:      0     242     452    1362 
   Size:        242     210     910

  Fragments arranged by size: 

                 910     242     210
 
BssHII G'CGCG_C

Cuts at:      0     508    1362 
   Size:        508     854
 
BstEII G'GTnAC_C

Cuts at:      0     348    1362 
   Size:        348    1014
 
BstYI r'GATC_y

Cuts at:      0     582    1184    1362 
   Size:        582     602     178

  Fragments arranged by size: 

                 602     582     178
 
BtgI C'CryG_G

Cuts at:      0     326     540    1362 
   Size:        326     214     822

  Fragments arranged by size: 

                 822     326     214
 
ClaI AT'CG_AT

Cuts at:      0     830    1362 
   Size:        830     532
 
EaeI y'GGCC_r

Cuts at:      0     329     461     543    1362 
   Size:        329     132      82     819

  Fragments arranged by size: 

                 819     329     132      82
 
EagI C'GGCC_G

Cuts at:      0     329     461    1362 
   Size:        329     132     901

  Fragments arranged by size: 

                 901     329     132
 
EarI CTCTTCn'nnn_

Cuts at:      0    1286    1362 
   Size:       1286      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     549     666     917    1310    1362 
   Size:        361     188     117     251     393      52

  Fragments arranged by size: 

                 393     361     251     188     117      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1082    1212    1362 
   Size:       1082     130     150

  Fragments arranged by size: 

                1082     150     130
 
EcoRV GAT'ATC

Cuts at:      0     189    1362 
   Size:        189    1173
 
HaeII r_GCGC'y

Cuts at:      0     178     593     689     979    1137    1362 
   Size:        178     415      96     290     158     225

  Fragments arranged by size: 

                 415     290     225     178     158      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     637     669     868     900    1362 
   Size:         79      32     526      32     199      32     462

  Fragments arranged by size: 

                 526     462     199      79      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     174    1362 
   Size:        174    1188
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1004    1175    1362 
   Size:       1004     171     187

  Fragments arranged by size: 

                1004     187     171
 
MspA1I CmG'CkG

Cuts at:      0     339     521     542    1362 
   Size:        339     182      21     820

  Fragments arranged by size: 

                 820     339     182      21
 
NarI GG'CG_CC

Cuts at:      0     175    1362 
   Size:        175    1187
 
PfoI T'CCnGG_A

Cuts at:      0     434    1187    1362 
   Size:        434     753     175

  Fragments arranged by size: 

                 753     434     175
 
PpuMI rG'GwC_Cy

Cuts at:      0    1082    1362 
   Size:       1082     280
 
PstI C_TGCA'G

Cuts at:      0     167     581    1362 
   Size:        167     414     781

  Fragments arranged by size: 

                 781     414     167
 
PvuII CAG'CTG

Cuts at:      0     339    1362 
   Size:        339    1023
 
SacII CC_GC'GG

Cuts at:      0     543    1362 
   Size:        543     819
 
SbfI CC_TGCA'GG

Cuts at:      0     581    1362 
   Size:        581     781
 
SfcI C'TryA_G

Cuts at:      0     163     577    1362 
   Size:        163     414     785

  Fragments arranged by size: 

                 785     414     163
 
SfoI GGC'GCC

Cuts at:      0     176    1362 
   Size:        176    1186
 
SmlI C'TyrA_G

Cuts at:      0     934    1362 
   Size:        934     428
 
StyI C'CwwG_G

Cuts at:      0    1217    1362 
   Size:       1217     145
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     367    1362 
   Size:        367     995
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     359     391     430     462    1341    1362 
   Size:        359      32      39      32     879      21

  Fragments arranged by size: 

                 879     359      39      32      32      21
 
Tth111I GACn'n_nGTC

Cuts at:      0     726    1030    1362 
   Size:        726     304     332

  Fragments arranged by size: 

                 726     332     304
 
XmnI GAAnn'nnTTC

Cuts at:      0     199    1362 
   Size:        199    1163
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI   BssHII   BstEII    BstYI     BtgI     ClaI     EaeI     EagI
     EarI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     KasI     MmeI
   MspA1I     NarI     PfoI    PpuMI     PstI    PvuII    SacII     SbfI
     SfcI     SfoI     SmlI     StyI    TaqII     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI
     XcmI     XhoI     XmaI     ZraI
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