Restriction map for pF1KB0499
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AhdI        
AlwNI       Aor51HI     AscI        AseI        AsnI        
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HindIII     KasI        MluI        MroI        MslI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AatII         503  GACGTC               GACGT^C
Acc65I        223  GGTACC               G^GTACC
AccI         1383  GTCTAC               GT^[AC][GT]AC
AccII          21  CGCG                 CG^CG
AcyI          503  GACGTC               G[GA]^CG[CT]C
AflIII        802  ACGTGT               A^C[GA][CT]GT
Alw44I        488  GTGCAC               G^TGCAC
AocI          973  CCTCAGG              CC^T[ACGT]AGG
ApaI           24  GGGCCC               GGGCC^C
ApaLI         488  GTGCAC               G^TGCAC
Asp700I      1181  GAATGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       223  GGTACC               G^GTACC
AspHI         488  GTGCAC               G[AT]GC[AT]^C
AvaII        1015  GGACC                G^G[AT]CC
BalI         1667  TGGCCA               TGG^CCA
BanI          223  GGTACC               G^G[CT][GA]CC
BanII          24  GGGCCC               G[GA]GC[CT]^C
BclI          111  TGATCA               T^GATCA
BglII        2118  AGATCT               A^GATCT
BsaAI        1087  TACGTA               [CT]AC^GT[GA]
BsaBI        1269  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         503  GACGTC               G[GA]^CG[CT]C
BseRI          35  GAGGAG               GAGGAG(10/8)
BsiHKAI       488  GTGCAC               G[AT]GC[AT]^C
BspHI         947  TCATGA               T^CATGA
BspMI        1482  ACCTGC               ACCTGC(4/8)
BstUI          21  CGCG                 CG^CG
BstYI        2118  AGATCT               [GA]^GATC[CT]
Bsu36I        973  CCTCAGG              CC^T[ACGT]AGG
CfoI         1064  GCGC                 GCG^C
DsaI           20  CCGCGG               C^C[GA][CT]GG
EaeI         1667  TGGCCA               [CT]^GGCC[GA]
Eco81I        973  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2080  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         535  GAATTC               G^AATTC
EcoT14I      1777  CCAAGG               C^C[AT][AT]GG
FbaI          111  TGATCA               T^GATCA
HaeII        1063  AGCGCC               [GA]GCGC^[CT]
HgaI         1195  GACGC                GACGC(5/10)
HhaI         1064  GCGC                 GCG^C
Hin1I         503  GACGTC               G[GA]^CG[CT]C
HinP1I       1064  GCGC                 G^CGC
HpaI          141  GTTAAC               GTT^AAC
KpnI          223  GGTACC               GGTAC^C
KspI           20  CCGCGG               CCGC^GG
MamI         1269  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2118  AGATCT               [GA]^GATC[CT]
MluNI        1667  TGGCCA               TGG^CCA
MscI         1667  TGGCCA               TGG^CCA
MspA1I         20  CCGCGG               C[AC]G^C[GT]G
MvnI           21  CGCG                 CG^CG
PflMI         607  CCATCTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1622  CTGCAG               CTGCA^G
RcaI          947  TCATGA               T^CATGA
SacII          20  CCGCGG               CCGC^GG
SapI          347  GCTCTTC              GCTCTTC(1/4)
SfcI         1622  CTGCAG               C^T[GA][CT]AG
SnaBI        1087  TACGTA               TAC^GTA
SphI          343  GCATGC               GCATG^C
SspI          524  AATATT               AAT^ATT
StyI         1777  CCAAGG               C^C[AT][AT]GG
Tsp45I       2071  GTCAC                ^GT[GC]AC
Van91I        607  CCATCTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        2118  AGATCT               [GA]^GATC[CT]
XmnI         1181  GAATGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwI          970  GATCC                GGATC(4/5)
             2113  GGATC               
BbsI         1882  GAAGAC               GAAGAC(2/6)
             1425  GTCTTC              
BcnI           27  CCCGG                CC^[GC]GG
              796  CCGGG               
BfaI          746  CTAG                 C^TAG
             2155  CTAG                
BmyI           24  GGGCCC               G[AGT]GC[ACT]^C
              488  GTGCAC              
BpmI         1010  CTCCAG               CTGGAG(16/14)
             2081  CTGGAG              
BpuAI        1882  GAAGAC               GAAGAC(2/6)
             1425  GTCTTC              
BsaJI          20  CCGCGG               C^C[ACGT][ACGT]GG
             1777  CCAAGG              
Bsp1286I       24  GGGCCC               G[AGT]GC[ACT]^C
              488  GTGCAC              
BstNI        1012  CCAGG                CC^[AT]GG
             2080  CCTGG               
EcoRII       1012  CCAGG                ^CC[AT]GG
             2080  CCTGG               
FauI           16  CCCGC                CCCGC(4/6)
               22  GCGGG               
HapII          28  CCGG                 C^CGG
              796  CCGG                
HincII        141  GTTAAC               GT[CT]^[GA]AC
             2039  GTCAAC              
HindII        141  GTTAAC               GT[CT]^[GA]AC
             2039  GTCAAC              
HpaII          28  CCGG                 C^CGG
              796  CCGG                
MaeI          746  CTAG                 C^TAG
             2155  CTAG                
MfeI          423  CAATTG               C^AATTG
              509  CAATTG              
MspI           28  CCGG                 C^CGG
              796  CCGG                
MunI          423  CAATTG               C^AATTG
              509  CAATTG              
MvaI         1012  CCAGG                CC^[AT]GG
             2080  CCTGG               
NciI           27  CCCGG                CC^[GC]GG
              796  CCGGG               
NspI          343  GCATGC               [GA]CATG^[CT]
             2196  ACATGC              
SfaNI        1944  GATGC                GCATC(5/9)
             1518  GCATC               
SmlI         1844  CTCAAG               C^T[CT][GA]AG
             1876  CTCAAG              
TaqI          118  TCGA                 T^CGA
             2139  TCGA                
TthHB8I       118  TCGA                 T^CGA
             2139  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BsiYI       BslI        BsmAI       BsmI        
BsrI        BssKI       BstF5I      Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       TfiI        Tru9I       TseI        Tsp509I     
TspRI       
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