Restriction map for
pF1KE0097
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 622 GTAC GT^AC
AseI 252 ATTAAT AT^TAAT
AsnI 252 ATTAAT AT^TAAT
Asp700I 723 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 21 GAGCTC G[AT]GC[AT]^C
AvaII 811 GGTCC G^G[AT]CC
BanII 21 GAGCTC G[GA]GC[CT]^C
BbsI 505 GAAGAC GAAGAC(2/6)
BciVI 573 GTATCC GTATCC(6/5)
BglII 57 AGATCT A^GATCT
BlpI 652 GCTGAGC GC^T[ACGT]AGC
BmyI 21 GAGCTC G[AGT]GC[ACT]^C
BpmI 478 CTCCAG CTGGAG(16/14)
Bpu1102I 652 GCTGAGC GC^T[ACGT]AGC
BpuAI 505 GAAGAC GAAGAC(2/6)
BsgI 487 GTGCAG GTGCAG(16/14)
BsiHKAI 21 GAGCTC G[AT]GC[AT]^C
Bsp1286I 21 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 621 TGTACA T^GTACA
BspHI 584 TCATGA T^CATGA
BsrGI 621 TGTACA T^GTACA
BsrI 480 CCAGT ACTGG(1/-1)
Cac8I 598 GCTTGC GC[ACGT]^[ACGT]GC
CelII 652 GCTGAGC GC^T[ACGT]AGC
CfoI 72 GCGC GCG^C
EcoRI 415 GAATTC G^AATTC
HaeII 71 AGCGCC [GA]GCGC^[CT]
HaeIII 499 GGCC GG^CC
HhaI 72 GCGC GCG^C
HinP1I 72 GCGC G^CGC
HphI 154 GGTGA GGTGA(8/7)
MaeIII 117 GTTAC ^GT[ACGT]AC
NdeI 362 CATATG CA^TATG
PflMI 638 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 252 ATTAAT AT^TAAT
RcaI 584 TCATGA T^CATGA
RsaI 622 GTAC GT^AC
SacI 21 GAGCTC GAGCT^C
SspBI 621 TGTACA T^GTACA
TaqI 731 TCGA T^CGA
TthHB8I 731 TCGA T^CGA
Van91I 638 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 723 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 244 GATCC GGATC(4/5)
558 GGATC
BbvI 328 GCTGC GCAGC(8/12)
331 GCTGC
BfaI 353 CTAG C^TAG
833 CTAG
BsiYI 521 CCCTTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
638 CCAGGCAATGG
BslI 521 CCCTTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
638 CCAGGCAATGG
BsrDI 36 GCAATG GCAATG(2/0)
642 GCAATG
BssKI 221 CCAGG ^CC[ACGT]GG
638 CCAGG
BstNI 221 CCAGG CC^[AT]GG
638 CCAGG
Cfr13I 498 GGGCC G^G[ACGT]CC
811 GGTCC
DraI 84 TTTAAA TTT^AAA
702 TTTAAA
EcoRII 221 CCAGG ^CC[AT]GG
638 CCAGG
Fnu4HI 328 GCTGC GC^[ACGT]GC
331 GCTGC
HinfI 212 GATTC G^A[ACGT]TC
396 GAATC
ItaI 328 GCTGC GC^[ACGT]GC
331 GCTGC
MaeI 353 CTAG C^TAG
833 CTAG
MvaI 221 CCAGG CC^[AT]GG
638 CCAGG
NlaIV 258 GGCTCC GG[ACGT]^[ACGT]CC
810 GGGTCC
Sau96I 498 GGGCC G^G[ACGT]CC
811 GGTCC
ScrFI 221 CCAGG CC^[ACGT]GG
638 CCAGG
SfcI 549 CTACAG C^T[GA][CT]AG
837 CTACAG
SspI 107 AATATT AAT^ATT
123 AATATT
TfiI 212 GATTC G^A[AT]TC
396 GAATC
TseI 328 GCTGC G^C[AT]GC
331 GCTGC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
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AcsI AflIII AluI ApoI BspLU11I
BstF5I BstYI DdeI DpnII FokI
MboI MboII MflI MnlI MseI
NdeII NlaIII NspI Sau3AI SfaNI
Tru9I Tsp509I TspRI XhoII