(Linear) (Six-base) MAPSORT of: KIEE0097.seq  Check: 4277  from: 1  to: 849



KIEE0097.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AflIII A'CryG_T

Cuts at:      0     265     509     606     849 
   Size:        265     244      97     243

  Fragments arranged by size: 

                 265     244     243      97
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     650     665     849 
   Size:        650      15     184

  Fragments arranged by size: 

                 650     184      15
 
ApoI r'AATT_y

Cuts at:      0     295     379     415     726     849 
   Size:        295      84      36     311     123

  Fragments arranged by size: 

                 311     295     123      84      36
 
AseI AT'TA_AT

Cuts at:      0     253     849 
   Size:        253     596
 
BanII G_rGCy'C

Cuts at:      0      25     849 
   Size:         25     824
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     512     849 
   Size:        512     337
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     584     849 
   Size:        584     265
 
BglII A'GATC_T

Cuts at:      0      57     849 
   Size:         57     792
 
BlpI GC'TnA_GC

Cuts at:      0     653     849 
   Size:        653     196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     849 
   Size:        463     386
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     461     491     849 
   Size:        461      30     358

  Fragments arranged by size: 

                 461     358      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     849 
   Size:        508     341
 
BsiHKAI G_wGCw'C

Cuts at:      0      25     849 
   Size:         25     824
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     849 
   Size:         25     824
 
BspHI T'CATG_A

Cuts at:      0     584     849 
   Size:        584     265
 
BsrDI GCAATG_nn'

Cuts at:      0      43     649     849 
   Size:         43     606     200

  Fragments arranged by size: 

                 606     200      43
 
BsrGI T'GTAC_A

Cuts at:      0     621     849 
   Size:        621     228
 
BstYI r'GATC_y

Cuts at:      0      57     243     558     849 
   Size:         57     186     315     291

  Fragments arranged by size: 

                 315     291     186      57
 
BtsI GCAGTG_nn'

Cuts at:      0     188     724     849 
   Size:        188     536     125

  Fragments arranged by size: 

                 536     188     125
 
DraI TTT'AAA

Cuts at:      0      86     704     849 
   Size:         86     618     145

  Fragments arranged by size: 

                 618     145      86
 
EcoICRI GAG'CTC

Cuts at:      0      23     849 
   Size:         23     826
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     849 
   Size:        463     386
 
EcoRI G'AATT_C

Cuts at:      0     415     849 
   Size:        415     434
 
HaeII r_GCGC'y

Cuts at:      0      75     849 
   Size:         75     774
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     441     461     473     493     849 
   Size:        441      20      12      20     356

  Fragments arranged by size: 

                 441     356      20      20      12
 
NdeI CA'TA_TG

Cuts at:      0     363     849 
   Size:        363     486
 
NspI r_CATG'y

Cuts at:      0     269     513     610     849 
   Size:        269     244      97     239

  Fragments arranged by size: 

                 269     244     239      97
 
PciI A'CATG_T

Cuts at:      0     265     509     606     849 
   Size:        265     244      97     243

  Fragments arranged by size: 

                 265     244     243      97
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     644     849 
   Size:        644     205
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     820     849 
   Size:        820      29
 
SacI G_AGCT'C

Cuts at:      0      25     849 
   Size:         25     824
 
SfcI C'TryA_G

Cuts at:      0     549     837     849 
   Size:        549     288      12

  Fragments arranged by size: 

                 549     288      12
 
SspI AAT'ATT

Cuts at:      0     109     125     849 
   Size:        109      16     724

  Fragments arranged by size: 

                 724     109      16
 
TatI w'GTAC_w

Cuts at:      0     621     849 
   Size:        621     228
 
XmnI GAAnn'nnTTC

Cuts at:      0     727     849 
   Size:        727     122
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI     AseI    BanII     BbsI    BciVI    BglII
     BlpI     BpmI    BsaXI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI
    BsrGI    BstYI     BtsI     DraI  EcoICRI  Eco57MI    EcoRI    HaeII
    Hin4I     NdeI     NspI     PciI    PflMI     PsrI     SacI     SfcI
     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI