################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 18 GACGTC GACGT^C AcyI 18 GACGTC G[GA]^CG[CT]C AlwI 489 GATCC GGATC(4/5) AocI 354 CCTCAGG CC^T[ACGT]AGG AspI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 608 TGCGCA TGC^GCA BalI 146 TGGCCA TGG^CCA BanI 73 GGTGCC G^G[CT][GA]CC BanII 340 GAGCCC G[GA]GC[CT]^C BbsI 463 GAAGAC GAAGAC(2/6) BcnI 14 CCCGG CC^[GC]GG BmyI 340 GAGCCC G[AGT]GC[ACT]^C BpuAI 463 GAAGAC GAAGAC(2/6) BsaBI 403 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 18 GACGTC G[GA]^CG[CT]C BsgI 616 GTGCAG GTGCAG(16/14) BsmAI 598 GAGAC GTCTC(1/5) BsmFI 21 GTCCC GGGAC(10/14) Bsp1286I 340 GAGCCC G[AGT]GC[ACT]^C BsrBI 624 CCGCTC CCGCTC(-3/-3) BsrI 481 ACTGG ACTGG(1/-1) BssHII 134 GCGCGC G^CGCGC BstF5I 438 CATCC GGATG(2/0) BstXI 371 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 488 AGATCC [GA]^GATC[CT] Bsu36I 354 CCTCAGG CC^T[ACGT]AGG DpnII 489 GATC ^GATC DraII 422 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 146 TGGCCA [CT]^GGCC[GA] Eco81I 354 CCTCAGG CC^T[ACGT]AGG EcoNI 414 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 422 AGGACCT [GA]G^G[ACGT]CC[CT] FokI 438 CATCC GGATG(9/13) FspI 608 TGCGCA TGC^GCA HaeIII 147 GGCC GG^CC HgaI 538 GACGC GACGC(5/10) Hin1I 18 GACGTC G[GA]^CG[CT]C HphI 411 TCACC GGTGA(8/7) MaeII 19 ACGT A^CGT MamI 403 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 489 GATC ^GATC MflI 488 AGATCC [GA]^GATC[CT] MluNI 146 TGGCCA TGG^CCA MscI 146 TGGCCA TGG^CCA MslI 529 CACAACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 14 CCCGG CC^[GC]GG NdeII 489 GATC ^GATC PflFI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 422 AGGACCT [GA]G^G[AT]CC[CT] Sau3AI 489 GATC ^GATC SexAI 377 ACCTGGT A^CC[AT]GGT SfcI 504 CTACAG C^T[GA][CT]AG Tth111I 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 294 TCTAGA T^CTAGA XhoII 488 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 133 CGCG CG^CG 135 CGCG AvaII 192 GGACC G^G[AT]CC 423 GGACC BfaI 295 CTAG C^TAG 427 CTAG BpmI 5 CTCCAG CTGGAG(16/14) 337 CTGGAG BsaJI 55 CCCTGG C^C[ACGT][ACGT]GG 392 CCTCGG BstUI 133 CGCG CG^CG 135 CGCG Cfr13I 192 GGACC G^G[ACGT]CC 423 GGACC DdeI 240 CTCAG C^T[ACGT]AG 355 CTCAG HapII 15 CCGG C^CGG 477 CCGG HpaII 15 CCGG C^CGG 477 CCGG MaeI 295 CTAG C^TAG 427 CTAG MboII 172 GAAGA GAAGA(8/7) 463 GAAGA MspA1I 142 CAGCTG C[AC]G^C[GT]G 639 CAGCTG MspI 15 CCGG C^CGG 477 CCGG MvnI 133 CGCG CG^CG 135 CGCG PvuII 142 CAGCTG CAG^CTG 639 CAGCTG Sau96I 192 GGACC G^G[ACGT]CC 423 GGACC SfaNI 308 GCATC GCATC(5/9) 437 GCATC SmlI 493 CTCAAG C^T[CT][GA]AG 547 CTCAAG TaqI 326 TCGA T^CGA 596 TCGA TspRI 542 CACTG CA[GC]TG[ACGT][ACGT]^ 619 CAGTG TthHB8I 326 TCGA T^CGA 596 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BbvI BsiYI BslI BssKI BstNI Cac8I CfoI EcoRII Fnu4HI HhaI HinP1I HinfI ItaI MnlI MvaI MwoI NlaIII NlaIV PleI ScrFI TseI