Restriction map for pF1KB6997
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MboI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MvnI        NaeI        NarI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        Sau3AI      SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwNI         292  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
BcnI          503  CCCGG                CC^[GC]GG
BfaI          200  CTAG                 C^TAG
BglI          437  GCCTGACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          345  CTCCAG               CTGGAG(16/14)
BsmI          315  GAATGC               GAATGC(1/-1)
BstF5I        561  CATCC                GGATG(2/0)
DraII          21  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          389  CTCTTC               CTCTTC(1/4)
EcoNI         450  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       21  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       209  CCATGG               C^C[AT][AT]GG
FauI          464  CCCGC                CCCGC(4/6)
FokI          561  CATCC                GGATG(9/13)
HapII         504  CCGG                 C^CGG
HgaI          260  GACGC                GACGC(5/10)
HpaII         504  CCGG                 C^CGG
Ksp632I       389  CTCTTC               CTCTTC(1/4)
MaeI          200  CTAG                 C^TAG
MfeI          369  CAATTG               C^AATTG
MspI          504  CCGG                 C^CGG
MunI          369  CAATTG               C^AATTG
NciI          503  CCCGG                CC^[GC]GG
NcoI          209  CCATGG               C^CATGG
PflMI         603  CCAGTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          268  GACTC                GAGTC(4/5)
PpuMI          21  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          298  CTGCAG               CTGCA^G
SapI          388  GCTCTTC              GCTCTTC(1/4)
ScaI          326  AGTACT               AGT^ACT
SfcI          298  CTGCAG               C^T[GA][CT]AG
StyI          209  CCATGG               C^C[AT][AT]GG
TfiI          620  GATTC                G^A[AT]TC
Tsp509I       370  AATT                 ^AATT
TspRI         493  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        603  CCAGTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          209  CCATGGGGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          327  GTAC                 GT^AC
              613  GTAC                
AvaII          22  GGTCC                G^G[AT]CC
              556  GGTCC               
BseRI          70  GAGGAG               GAGGAG(10/8)
               94  GAGGAG              
BsrDI         498  CATTGC               GCAATG(2/0)
              551  GCAATG              
BsrI          442  ACTGG                ACTGG(1/-1)
              603  CCAGT               
BstAPI        291  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              310  GCAGAGAATGC         
BstEII        180  GGTCACC              G^GT[ACGT]ACC
              459  GGTGACC             
BstPI         180  GGTCACC              G^GT[ACGT]ACC
              459  GGTGACC             
Cac8I          48  GCCTGC               GC[ACGT]^[ACGT]GC
              231  GCTCGC              
DdeI           68  CTGAG                C^T[ACGT]AG
              270  CTCAG               
DsaI          209  CCATGG               C^C[GA][CT]GG
              305  CCACGG              
EcoO65I       180  GGTCACC              G^GT[ACGT]ACC
              459  GGTGACC             
HinfI         268  GACTC                G^A[ACGT]TC
              620  GATTC               
MaeII         113  ACGT                 A^CGT
              569  ACGT                
MaeIII        181  GTCAC                ^GT[ACGT]AC
              460  GTGAC               
NlaIV         374  GGGGCC               GG[ACGT]^[ACGT]CC
              556  GGTCCC              
RsaI          327  GTAC                 GT^AC
              613  GTAC                
SfaNI         365  GCATC                GCATC(5/9)
              489  GCATC               
Tsp45I        181  GTCAC                ^GT[GC]AC
              460  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BsmFI       BspMI       BssKI       BstNI       
Cfr13I      EcoRII      Fnu4HI      HaeIII      HphI        
ItaI        MboII       MnlI        MvaI        MwoI        
NlaIII      Sau96I      ScrFI       TseI        
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