Restriction map for pF1KA1508
 (Linear) (Six-base) MAPSORT of: KIAA1508.seq  Check: 8064  from: 1  to: 3378



KIAA1508.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     654    2179    3378 
   Size:        654    1525    1199

  Fragments arranged by size: 

                1525    1199     654
 
AatII G_ACGT'C

Cuts at:      0    3148    3378 
   Size:       3148     230
 
Acc65I G'GTAC_C

Cuts at:      0     212     831    3378 
   Size:        212     619    2547

  Fragments arranged by size: 

                2547     619     212
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1964    1986    3219    3378 
   Size:        187    1777      22    1233     159

  Fragments arranged by size: 

                1777    1233     187     159      22
 
AfeI AGC'GCT

Cuts at:      0    1804    2854    3378 
   Size:       1804    1050     524

  Fragments arranged by size: 

                1804    1050     524
 
AflIII A'CryG_T

Cuts at:      0    2267    3378 
   Size:       2267    1111
 
AgeI A'CCGG_T

Cuts at:      0     899    3378 
   Size:        899    2479
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3354    3378 
   Size:       3354      24
 
AleI CACnn'nnGTG

Cuts at:      0     565    3378 
   Size:        565    2813
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     790    1655    2095    3352    3378 
   Size:        790     865     440    1257      26

  Fragments arranged by size: 

                1257     865     790     440      26
 
AlwNI CAG_nnn'CTG

Cuts at:      0     763    1176    1678    2556    3378 
   Size:        763     413     502     878     822

  Fragments arranged by size: 

                 878     822     763     502     413
 
ApaI G_GGCC'C

Cuts at:      0      22     882    1145    1377    2459    3378 
   Size:         22     860     263     232    1082     919

  Fragments arranged by size: 

                1082     919     860     263     232      22
 
ApaLI G'TGCA_C

Cuts at:      0     154    3378 
   Size:        154    3224
 
ApoI r'AATT_y

Cuts at:      0    3043    3378 
   Size:       3043     335
 
AvaI C'yCGr_G

Cuts at:      0     142     542     627     875    1127    1186    1370    2152 
   Size:        142     400      85     248     252      59     184     782

Cuts at:   2152    2911    3351    3378 
   Size:        759     440      27

  Fragments arranged by size: 

                 782     759     440     400     252     248     184     142
                  85      59      27
 
AvrII C'CTAG_G

Cuts at:      0      40    3378 
   Size:         40    3338
 
BanI G'GyrC_C

Cuts at:      0      44     212     567     709     774     831     857     983 
   Size:         44     168     355     142      65      57      26     126

Cuts at:    983    1687    1862    1936    2205    3378 
   Size:        704     175      74     269    1173

  Fragments arranged by size: 

                1173     704     355     269     175     168     142     126
                  74      65      57      44      26
 
BanII G_rGCy'C

Cuts at:      0      22     227     578     635     737     882     993    1145 
   Size:         22     205     351      57     102     145     111     152

Cuts at:   1145    1377    1601    2153    2459    3378 
   Size:        232     224     552     306     919

  Fragments arranged by size: 

                 919     552     351     306     232     224     205     152
                 145     111     102      57      22
 
BbeI G_GCGC'C

Cuts at:      0      48    1866    2209    3378 
   Size:         48    1818     343    1169

  Fragments arranged by size: 

                1818    1169     343      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     266     343    1571    3378 
   Size:        266      77    1228    1807

  Fragments arranged by size: 

                1807    1228     266      77
 
BbvCI CC'TCA_GC

Cuts at:      0     582    2315    3378 
   Size:        582    1733    1063

  Fragments arranged by size: 

                1733    1063     582
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      57      91     973    1007    3378 
   Size:         57      34     882      34    2371

  Fragments arranged by size: 

                2371     882      57      34      34
 
BclI T'GATC_A

Cuts at:      0    2408    2873    3378 
   Size:       2408     465     505

  Fragments arranged by size: 

                2408     505     465
 
BglI GCCn_nnn'nGGC

Cuts at:      0     277     877     968     977    1172    1235    1552    2498 
   Size:        277     600      91       9     195      63     317     946

Cuts at:   2498    3378 
   Size:        880

  Fragments arranged by size: 

                 946     880     600     317     277     195      91      63
                   9
 
BlpI GC'TnA_GC

Cuts at:      0    2131    3378 
   Size:       2131    1247
 
Bme1580I G_kGCm'C

Cuts at:      0      22     158     660     779     862     882    1053    1145 
   Size:         22     136     502     119      83      20     171      92

Cuts at:   1145    1377    1690    1939    2459    2777    3299    3341    3378 
   Size:        232     313     249     520     318     522      42      37

  Fragments arranged by size: 

                 522     520     502     318     313     249     232     171
                 136     119      92      83      42      37      22      20
            
 
BmgBI CAC'GTC

Cuts at:      0    1635    3378 
   Size:       1635    1743
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      25     587    1048    1296    3378 
   Size:         25     562     461     248    2082

  Fragments arranged by size: 

                2082     562     461     248      25
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     597    3378 
   Size:        597    2781
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     406     494     639     811    1399    1641    2686 
   Size:        241     165      88     145     172     588     242    1045

Cuts at:   2686    2770    3378 
   Size:         84     608

  Fragments arranged by size: 

                1045     608     588     242     241     172     165     145
                  88      84
 
Bpu10I CC'TnA_GC

Cuts at:      0     572     582    2315    3378 
   Size:        572      10    1733    1063

  Fragments arranged by size: 

                1733    1063     572      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1029    2488    2503    2872    3046    3378 
   Size:       1029    1459      15     369     174     332

  Fragments arranged by size: 

                1459    1029     369     332     174      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     290    1659    2245    3378 
   Size:        290    1369     586    1133

  Fragments arranged by size: 

                1369    1133     586     290
 
BsaAI yAC'GTr

Cuts at:      0    2268    2536    3378 
   Size:       2268     268     842

  Fragments arranged by size: 

                2268     842     268
 
BsaHI Gr'CG_yC

Cuts at:      0      45     742    1728    1863    2206    3145    3378 
   Size:         45     697     986     135     343     939     233

  Fragments arranged by size: 

                 986     939     697     343     233     135      45
 
BsaWI w'CCGG_w

Cuts at:      0     899    1202    1898    3378 
   Size:        899     303     696    1480

  Fragments arranged by size: 

                1480     899     696     303
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     841     871    3378 
   Size:        841      30    2507

  Fragments arranged by size: 

                2507     841      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     209     717     922    1231    1764    2274    2683    3378 
   Size:        209     508     205     309     533     510     409     695

  Fragments arranged by size: 

                 695     533     510     508     409     309     209     205
            
 
BseYI C'CCAG_C

Cuts at:      0     700     781     861     966    1082    1091    1363    1609 
   Size:        700      81      80     105     116       9     272     246

Cuts at:   1609    1882    1931    2334    3378 
   Size:        273      49     403    1044

  Fragments arranged by size: 

                1044     700     403     273     272     246     116     105
                  81      80      49       9
 
BsiEI CG_ry'CG

Cuts at:      0     152    1357    2183    3378 
   Size:        152    1205     826    1195

  Fragments arranged by size: 

                1205    1195     826     152
 
BsiHKAI G_wGCw'C

Cuts at:      0      37     158     993    3378 
   Size:         37     121     835    2385

  Fragments arranged by size: 

                2385     835     121      37
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     846    3378 
   Size:        846    2532
 
Bsp1286I G_dGCh'C

Cuts at:      0      22      37     158     227     578     635     660     737 
   Size:         22      15     121      69     351      57      25      77

Cuts at:    737     779     862     882     993    1053    1145    1377    1601 
   Size:         42      83      20     111      60      92     232     224

Cuts at:   1601    1690    1939    2153    2459    2777    3299    3341    3378 
   Size:         89     249     214     306     318     522      42      37

  Fragments arranged by size: 

                 522     351     318     306     249     232     224     214
                 121     111      92      89      83      77      69      60
                  57      42      42      37      25      22      20      15
            
 
BspEI T'CCGG_A

Cuts at:      0    1898    3378 
   Size:       1898    1480
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     654    1142    1790    2179    2564    2849    3378 
   Size:        654     488     648     389     385     285     529

  Fragments arranged by size: 

                 654     648     529     488     389     385     285
 
BsrBI CCG'CTC

Cuts at:      0    1074    1772    2142    2641    3378 
   Size:       1074     698     370     499     737

  Fragments arranged by size: 

                1074     737     698     499     370
 
BsrFI r'CCGG_y

Cuts at:      0     299     899    1234    2157    2182    3378 
   Size:        299     600     335     923      25    1196

  Fragments arranged by size: 

                1196     923     600     335     299      25
 
BsrGI T'GTAC_A

Cuts at:      0      83    3378 
   Size:         83    3295
 
BssHII G'CGCG_C

Cuts at:      0    2116    3378 
   Size:       2116    1262
 
BssSI C'ACGA_G

Cuts at:      0    2230    2741    3378 
   Size:       2230     511     637

  Fragments arranged by size: 

                2230     637     511
 
BstEII G'GTnAC_C

Cuts at:      0    3171    3378 
   Size:       3171     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     789    2452    3378 
   Size:        789    1663     926

  Fragments arranged by size: 

                1663     926     789
 
BstYI r'GATC_y

Cuts at:      0    3012    3378 
   Size:       3012     366
 
Bsu36I CC'TnA_GG

Cuts at:      0    1417    2542    3287    3378 
   Size:       1417    1125     745      91

  Fragments arranged by size: 

                1417    1125     745      91
 
BtgI C'CryG_G

Cuts at:      0     189     227     866     928    1796    2684    2820    3378 
   Size:        189      38     639      62     868     888     136     558

  Fragments arranged by size: 

                 888     868     639     558     189     136      62      38
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1418    1832    3054    3378 
   Size:       1418     414    1222     324

  Fragments arranged by size: 

                1418    1222     414     324
 
BtsI GCAGTG_nn'

Cuts at:      0     156     586    2795    3299    3378 
   Size:        156     430    2209     504      79

  Fragments arranged by size: 

                2209     504     430     156      79
 
EaeI y'GGCC_r

Cuts at:      0     149    1354    1553    1764    1787    2180    3378 
   Size:        149    1205     199     211      23     393    1198

  Fragments arranged by size: 

                1205    1198     393     211     199     149      23
 
EagI C'GGCC_G

Cuts at:      0     149    1354    2180    3378 
   Size:        149    1205     826    1198

  Fragments arranged by size: 

                1205    1198     826     149
 
EarI CTCTTCn'nnn_

Cuts at:      0     170     678    3239    3378 
   Size:        170     508    2561     139

  Fragments arranged by size: 

                2561     508     170     139
 
EcoICRI GAG'CTC

Cuts at:      0     991    3378 
   Size:        991    2387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     241     406     494     639     811    1399    1641 
   Size:        187      54     165      88     145     172     588     242

Cuts at:   1641    1964    1986    2686    2770    3219    3378 
   Size:        323      22     700      84     449     159

  Fragments arranged by size: 

                 700     588     449     323     242     187     172     165
                 159     145      88      84      54      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     169     552    3378 
   Size:        169     383    2826

  Fragments arranged by size: 

                2826     383     169
 
EcoO109I rG'GnC_Cy

Cuts at:      0     199    1131    1141    1142    1149    1173    1374    1919 
   Size:        199     932      10       1       7      24     201     545

Cuts at:   1919    2382    2499    2553    3378 
   Size:        463     117      54     825

  Fragments arranged by size: 

                 932     825     545     463     201     199     117      54
                  24      10       7       1
 
EcoRV GAT'ATC

Cuts at:      0    2010    3378 
   Size:       2010    1368
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2913    3378 
   Size:       2913     465
 
HaeII r_GCGC'y

Cuts at:      0      48     890    1230    1806    1866    2209    2856    3378 
   Size:         48     842     340     576      60     343     647     522

  Fragments arranged by size: 

                 842     647     576     522     343     340      60      48
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51     330     362    2997    3029    3340    3372 
   Size:         19      32     279      32    2635      32     311      32

Cuts at:   3372    3378 
   Size:          6

  Fragments arranged by size: 

                2635     311     279      32      32      32      32      19
                   6
 
HindIII A'AGCT_T

Cuts at:      0    2419    3378 
   Size:       2419     959
 
KasI G'GCGC_C

Cuts at:      0      44    1862    2205    3378 
   Size:         44    1818     343    1173

  Fragments arranged by size: 

                1818    1173     343      44
 
KpnI G_GTAC'C

Cuts at:      0     216     835    3378 
   Size:        216     619    2543

  Fragments arranged by size: 

                2543     619     216
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     884    3350    3378 
   Size:        884    2466      28

  Fragments arranged by size: 

                2466     884      28
 
MscI TGG'CCA

Cuts at:      0    1555    1789    3378 
   Size:       1555     234    1589

  Fragments arranged by size: 

                1589    1555     234
 
MslI CAynn'nnrTG

Cuts at:      0     565    3029    3378 
   Size:        565    2464     349

  Fragments arranged by size: 

                2464     565     349
 
MspA1I CmG'CkG

Cuts at:      0       9     426     966    1452    2075    2287    2822    3361 
   Size:          9     417     540     486     623     212     535     539

Cuts at:   3361    3378 
   Size:         17

  Fragments arranged by size: 

                 623     540     539     535     486     417     212      17
                   9
 
NaeI GCC'GGC

Cuts at:      0    2159    2184    3378 
   Size:       2159      25    1194

  Fragments arranged by size: 

                2159    1194      25
 
NarI GG'CG_CC

Cuts at:      0      45    1863    2206    3378 
   Size:         45    1818     343    1172

  Fragments arranged by size: 

                1818    1172     343      45
 
NcoI C'CATG_G

Cuts at:      0     227     866    3378 
   Size:        227     639    2512

  Fragments arranged by size: 

                2512     639     227
 
NgoMIV G'CCGG_C

Cuts at:      0    2157    2182    3378 
   Size:       2157      25    1196

  Fragments arranged by size: 

                2157    1196      25
 
NspI r_CATG'y

Cuts at:      0    2334    2723    3183    3378 
   Size:       2334     389     460     195

  Fragments arranged by size: 

                2334     460     389     195
 
PasI CC'CwG_GG

Cuts at:      0    1343    2603    3378 
   Size:       1343    1260     775

  Fragments arranged by size: 

                1343    1260     775
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      28     763    3378 
   Size:         28     735    2615

  Fragments arranged by size: 

                2615     735      28
 
PfoI T'CCnGG_A

Cuts at:      0    1259    2354    3008    3378 
   Size:       1259    1095     654     370

  Fragments arranged by size: 

                1259    1095     654     370
 
PmlI CAC'GTG

Cuts at:      0    2268    2536    3378 
   Size:       2268     268     842

  Fragments arranged by size: 

                2268     842     268
 
PpuMI rG'GwC_Cy

Cuts at:      0     199    1131    1919    2382    2553    3378 
   Size:        199     932     788     463     171     825

  Fragments arranged by size: 

                 932     825     788     463     199     171
 
PsiI TTA'TAA

Cuts at:      0    2036    3378 
   Size:       2036    1342
 
PspOMI G'GGCC_C

Cuts at:      0      18     878    1141    1373    2455    3378 
   Size:         18     860     263     232    1082     923

  Fragments arranged by size: 

                1082     923     860     263     232      18
 
PstI C_TGCA'G

Cuts at:      0     163    1172    1750    2589    2846    3378 
   Size:        163    1009     578     839     257     532

  Fragments arranged by size: 

                1009     839     578     532     257     163
 
PvuII CAG'CTG

Cuts at:      0       9     426     966    1452    3378 
   Size:          9     417     540     486    1926

  Fragments arranged by size: 

                1926     540     486     417       9
 
RsrII CG'GwC_CG

Cuts at:      0    1232    3378 
   Size:       1232    2146
 
SacI G_AGCT'C

Cuts at:      0     993    3378 
   Size:        993    2385
 
SacII CC_GC'GG

Cuts at:      0    2823    3378 
   Size:       2823     555
 
SbfI CC_TGCA'GG

Cuts at:      0    1172    2846    3378 
   Size:       1172    1674     532

  Fragments arranged by size: 

                1674    1172     532
 
SexAI A'CCwGG_T

Cuts at:      0     827    3378 
   Size:        827    2551
 
SfcI C'TryA_G

Cuts at:      0     159    1168    1209    1746    2244    2585    2803    2842 
   Size:        159    1009      41     537     498     341     218      39

Cuts at:   2842    3378 
   Size:        536

  Fragments arranged by size: 

                1009     537     536     498     341     218     159      41
                  39
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     877    1172    1552    2498    3378 
   Size:        877     295     380     946     880

  Fragments arranged by size: 

                 946     880     877     380     295
 
SfoI GGC'GCC

Cuts at:      0      46    1864    2207    3378 
   Size:         46    1818     343    1171

  Fragments arranged by size: 

                1818    1171     343      46
 
SmaI CCC'GGG

Cuts at:      0     544     629     877    1372    2154    3353    3378 
   Size:        544      85     248     495     782    1199      25

  Fragments arranged by size: 

                1199     782     544     495     248      85      25
 
SmlI C'TyrA_G

Cuts at:      0    1008    2503    2518    2887    3061    3378 
   Size:       1008    1495      15     369     174     317

  Fragments arranged by size: 

                1495    1008     369     317     174      15
 
SphI G_CATG'C

Cuts at:      0    2723    3378 
   Size:       2723     655
 
SrfI GCCC'GGGC

Cuts at:      0     544    2154    3378 
   Size:        544    1610    1224

  Fragments arranged by size: 

                1610    1224     544
 
StuI AGG'CCT

Cuts at:      0     805    1330    3378 
   Size:        805     525    2048

  Fragments arranged by size: 

                2048     805     525
 
StyI C'CwwG_G

Cuts at:      0      40     227     404     677     866    1476    1922    3378 
   Size:         40     187     177     273     189     610     446    1456

  Fragments arranged by size: 

                1456     610     446     273     189     187     177      40
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      20    1356    3378 
   Size:         20    1336    2022

  Fragments arranged by size: 

                2022    1336      20
 
TatI w'GTAC_w

Cuts at:      0      83    3378 
   Size:         83    3295
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     732     764     841     873    3378 
   Size:        732      32      77      32    2505

  Fragments arranged by size: 

                2505     732      77      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     103     561    3378 
   Size:        103     458    2817

  Fragments arranged by size: 

                2817     458     103
 
XmaI C'CCGG_G

Cuts at:      0     542     627     875    1370    2152    3351    3378 
   Size:        542      85     248     495     782    1199      27

  Fragments arranged by size: 

                1199     782     542     495     248      85      27
 
ZraI GAC'GTC

Cuts at:      0    3146    3378 
   Size:       3146     232
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    HaeII    Hin4I  HindIII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI     PstI
    PvuII    RsrII     SacI    SacII     SbfI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII     AloI     AscI     AseI    AsiSI     BaeI
    BamHI    BciVI    BfrBI    BglII     BmtI    BsaBI     BsgI    BsiWI
     BsmI    BspHI    BsrDI   BstAPI    BstBI  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI     EciI    EcoRI     FseI     FspI    FspAI
   HincII     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI    PspXI     PsrI
     PvuI     SalI    SanDI     SapI     ScaI     SgfI    SgrAI    SnaBI
     SpeI     SspI     SwaI  Tth111I     XbaI     XhoI     XmnI
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