(Circular) (Six-base) MAPSORT of: pF1KB4917.seq Check: 110 from: 1 to: 4082 pF1KB4917 4082 bp With 182 enzymes: SgfI * April 24, 2009 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 187 460 187 Size: 273 3809 AccI GT'mk_AC Cuts at: 1022 1120 1022 Size: 98 3984 Acc65I G'GTAC_C Cuts at: 1098 1098 Size: 4082 AclI AA'CG_TT Cuts at: 3922 3922 Size: 4082 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 508 859 982 1907 2339 3086 3354 508 Size: 351 123 925 432 747 268 1236 Fragments arranged by size: 1236 925 747 432 351 268 123 AflIII A'CryG_T Cuts at: 785 1434 2538 3515 785 Size: 649 1104 977 1352 Fragments arranged by size: 1352 1104 977 649 AgeI A'CCGG_T Cuts at: 2470 2470 Size: 4082 AleI CACnn'nnGTG Cuts at: 181 181 Size: 4082 AlwNI CAG_nnn'CTG Cuts at: 408 1387 2954 408 Size: 979 1567 1536 Fragments arranged by size: 1567 1536 979 ApaI G_GGCC'C Cuts at: 949 949 Size: 4082 ApaLI G'TGCA_C Cuts at: 2852 2852 Size: 4082 ApoI r'AATT_y Cuts at: 1086 3435 3691 1086 Size: 2349 256 1477 Fragments arranged by size: 2349 1477 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4082 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4082 AvaI C'yCGr_G Cuts at: 340 676 1102 340 Size: 336 426 3320 Fragments arranged by size: 3320 426 336 BamHI G'GATC_C Cuts at: 908 1107 908 Size: 199 3883 BanI G'GyrC_C Cuts at: 448 571 967 1098 1512 1760 1795 448 Size: 123 396 131 414 248 35 2735 Fragments arranged by size: 2735 414 396 248 131 123 35 BanII G_rGCy'C Cuts at: 949 1096 2126 949 Size: 147 1030 2905 Fragments arranged by size: 2905 1030 147 BbeI G_GCGC'C Cuts at: 971 1516 1764 971 Size: 545 248 3289 Fragments arranged by size: 3289 545 248 BbsI GAAGACnn'nnnn_ Cuts at: 862 862 Size: 4082 BciVI GTATCCnnnnn_n' Cuts at: 1980 2741 4082 1980 Size: 761 1341 1980 Fragments arranged by size: 1980 1341 761 BclI T'GATC_A Cuts at: 1602 1602 Size: 4082 BglI GCCn_nnn'nGGC Cuts at: 829 829 Size: 4082 BglII A'GATC_T Cuts at: 1597 3634 1597 Size: 2037 2045 BlpI GC'TnA_GC Cuts at: 1194 1194 Size: 4082 Bme1580I G_kGCm'C Cuts at: 576 949 1707 1800 2856 576 Size: 373 758 93 1056 1802 Fragments arranged by size: 1802 1056 758 373 93 BmrI ACTGGGnnnn_n' Cuts at: 1294 1375 1710 3854 1294 Size: 81 335 2144 1522 Fragments arranged by size: 2144 1522 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 966 2449 966 Size: 1483 2599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 578 850 578 Size: 272 3810 Bpu10I CC'TnA_GC Cuts at: 691 691 Size: 4082 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1258 2137 2629 2927 1258 Size: 879 492 298 2413 Fragments arranged by size: 2413 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 667 3786 667 Size: 3119 963 BsaAI yAC'GTr Cuts at: 786 1435 786 Size: 649 3433 BsaBI GATnn'nnATC Cuts at: 6 1596 3633 6 Size: 1590 2037 455 Fragments arranged by size: 2037 1590 455 BsaHI Gr'CG_yC Cuts at: 760 884 968 1513 1761 2463 3959 760 Size: 124 84 545 248 702 1496 883 Fragments arranged by size: 1496 883 702 545 248 124 84 BsaWI w'CCGG_w Cuts at: 1271 1792 2470 2744 2891 3302 1271 Size: 521 678 274 147 411 2051 Fragments arranged by size: 2051 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 302 332 302 Size: 30 4052 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 781 781 Size: 4082 BseYI C'CCAG_C Cuts at: 575 620 747 811 1050 1507 2265 2842 3338 Size: 45 127 64 239 457 758 577 496 Cuts at: 3338 575 Size: 1319 Fragments arranged by size: 1319 758 577 496 457 239 127 64 45 BsiEI CG_ry'CG Cuts at: 104 1624 1670 2878 104 Size: 1520 46 1208 1308 Fragments arranged by size: 1520 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1096 1874 2064 2856 1096 Size: 778 190 792 2322 Fragments arranged by size: 2322 792 778 190 Bsp1286I G_dGCh'C Cuts at: 576 949 1096 1707 1800 1874 2064 2126 2856 Size: 373 147 611 93 74 190 62 730 Cuts at: 2856 576 Size: 1802 Fragments arranged by size: 1802 730 611 373 190 147 93 74 62 BspEI T'CCGG_A Cuts at: 3302 3302 Size: 4082 BspHI T'CATG_A Cuts at: 3258 3258 Size: 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 187 460 644 1132 1346 1648 2029 187 Size: 273 184 488 214 302 381 2240 Fragments arranged by size: 2240 488 381 302 273 214 184 BsrBI CCG'CTC Cuts at: 50 281 726 2374 2428 3912 4077 50 Size: 231 445 1648 54 1484 165 55 Fragments arranged by size: 1648 1484 445 231 165 55 54 BsrDI GCAATG_nn' Cuts at: 515 653 1994 515 Size: 138 1341 2603 Fragments arranged by size: 2603 1341 138 BsrFI r'CCGG_y Cuts at: 658 799 2080 2261 2470 658 Size: 141 1281 181 209 2270 Fragments arranged by size: 2270 1281 209 181 141 BsrGI T'GTAC_A Cuts at: 242 242 Size: 4082 BssHII G'CGCG_C Cuts at: 980 980 Size: 4082 BssSI C'ACGA_G Cuts at: 246 940 2353 2711 246 Size: 694 1413 358 1617 Fragments arranged by size: 1617 1413 694 358 BstAPI GCAn_nnn'nTGC Cuts at: 1459 1459 Size: 4082 BstBI TT'CG_AA Cuts at: 2443 2443 Size: 4082 BstXI CCAn_nnnn'nTGG Cuts at: 198 357 477 198 Size: 159 120 3803 Fragments arranged by size: 3803 159 120 BstYI r'GATC_y Cuts at: 1 491 533 908 929 1107 1574 1597 1932 Size: 490 42 375 21 178 467 23 335 Cuts at: 1932 2178 3190 3276 3288 3634 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 490 467 449 375 346 335 246 178 86 42 23 21 12 BtgI C'CryG_G Cuts at: 107 476 611 648 107 Size: 369 135 37 3541 Fragments arranged by size: 3541 369 135 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 141 975 993 1020 2032 2213 2376 141 Size: 834 18 27 1012 181 163 1847 Fragments arranged by size: 1847 1012 834 181 163 27 18 BtsI GCAGTG_nn' Cuts at: 1325 1325 Size: 4082 DraI TTT'AAA Cuts at: 1081 1081 Size: 4082 DrdI GACnn_nn'nnGTC Cuts at: 1788 2646 1788 Size: 858 3224 EaeI y'GGCC_r Cuts at: 200 231 614 797 830 1667 1841 2232 2259 Size: 31 383 183 33 837 174 391 27 Cuts at: 2259 3604 200 Size: 1345 678 Fragments arranged by size: 1345 837 678 391 383 183 174 33 31 27 EagI C'GGCC_G Cuts at: 1667 1667 Size: 4082 EarI CTCTTCn'nnn_ Cuts at: 2105 2315 2105 Size: 210 3872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2290 2600 2746 3892 2290 Size: 310 146 1146 2480 Fragments arranged by size: 2480 1146 310 146 EcoICRI GAG'CTC Cuts at: 1094 1094 Size: 4082 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 508 578 850 859 982 1907 2339 3086 3354 Size: 70 272 9 123 925 432 747 268 Cuts at: 3354 508 Size: 1236 Fragments arranged by size: 1236 925 747 432 272 268 123 70 9 EcoO109I rG'GnC_Cy Cuts at: 945 946 1065 1221 945 Size: 1 119 156 3806 Fragments arranged by size: 3806 156 119 1 EcoRI G'AATT_C Cuts at: 1086 1086 Size: 4082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 4082 FspI TGC'GCA Cuts at: 1863 1863 Size: 4082 HaeII r_GCGC'y Cuts at: 971 1516 1764 2786 3765 971 Size: 545 248 1022 979 1288 Fragments arranged by size: 1288 1022 979 545 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 371 403 757 789 886 918 1924 1954 1956 Size: 32 354 32 97 32 1006 30 2 Cuts at: 1956 1986 371 Size: 30 2467 Fragments arranged by size: 2467 1006 354 97 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 837 888 1121 837 Size: 51 233 3798 Fragments arranged by size: 3798 233 51 KasI G'GCGC_C Cuts at: 967 1512 1760 967 Size: 545 248 3289 Fragments arranged by size: 3289 545 248 KpnI G_GTAC'C Cuts at: 1102 1102 Size: 4082 MluI A'CGCG_T Cuts at: 3515 3515 Size: 4082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 281 402 2753 2937 281 Size: 121 2351 184 1426 Fragments arranged by size: 2351 1426 184 121 MscI TGG'CCA Cuts at: 202 1843 202 Size: 1641 2441 MslI CAynn'nnrTG Cuts at: 181 250 475 481 2071 2198 181 Size: 69 225 6 1590 127 2065 Fragments arranged by size: 2065 1590 225 127 69 6 MspA1I CmG'CkG Cuts at: 498 598 624 747 826 1193 1507 1867 2880 Size: 100 26 123 79 367 314 360 1013 Cuts at: 2880 3125 498 Size: 245 1455 Fragments arranged by size: 1455 1013 367 360 314 245 123 100 79 26 NaeI GCC'GGC Cuts at: 801 2263 801 Size: 1462 2620 NarI GG'CG_CC Cuts at: 968 1513 1761 968 Size: 545 248 3289 Fragments arranged by size: 3289 545 248 NcoI C'CATG_G Cuts at: 107 476 107 Size: 369 3713 NgoMIV G'CCGG_C Cuts at: 799 2261 799 Size: 1462 2620 NspI r_CATG'y Cuts at: 255 303 1135 1635 2542 255 Size: 48 832 500 907 1795 Fragments arranged by size: 1795 907 832 500 48 PasI CC'CwG_GG Cuts at: 546 564 546 Size: 18 4064 PciI A'CATG_T Cuts at: 2538 2538 Size: 4082 PfoI T'CCnGG_A Cuts at: 911 911 Size: 4082 PmeI GTTT'AAAC Cuts at: 1081 1081 Size: 4082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3221 3253 3221 Size: 32 4050 PpuMI rG'GwC_Cy Cuts at: 1065 1065 Size: 4082 PshAI GACnn'nnGTC Cuts at: 331 331 Size: 4082 PspOMI G'GGCC_C Cuts at: 945 945 Size: 4082 PstI C_TGCA'G Cuts at: 218 503 1064 1129 1814 218 Size: 285 561 65 685 2486 Fragments arranged by size: 2486 685 561 285 65 PvuI CG_AT'CG Cuts at: 104 104 Size: 4082 PvuII CAG'CTG Cuts at: 498 598 624 747 1507 1867 498 Size: 100 26 123 760 360 2713 Fragments arranged by size: 2713 760 360 123 100 26 RsrII CG'GwC_CG Cuts at: 2277 2277 Size: 4082 SacI G_AGCT'C Cuts at: 1096 1096 Size: 4082 SalI G'TCGA_C Cuts at: 1119 1119 Size: 4082 SapI GCTCTTCn'nnn_ Cuts at: 2105 2315 2105 Size: 210 3872 SbfI CC_TGCA'GG Cuts at: 1129 1129 Size: 4082 ScaI AGT'ACT Cuts at: 3599 3599 Size: 4082 SfcI C'TryA_G Cuts at: 33 214 499 1060 1125 1810 2803 2994 33 Size: 181 285 561 65 685 993 191 1121 Fragments arranged by size: 1121 993 685 561 285 191 181 65 SfoI GGC'GCC Cuts at: 969 1514 1762 969 Size: 545 248 3289 Fragments arranged by size: 3289 545 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4082 SmaI CCC'GGG Cuts at: 1104 1104 Size: 4082 SmlI C'TyrA_G Cuts at: 1237 2152 2644 2906 1237 Size: 915 492 262 2413 Fragments arranged by size: 2413 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4082 SphI G_CATG'C Cuts at: 1135 1635 1135 Size: 500 3582 StyI C'CwwG_G Cuts at: 107 476 1216 1570 107 Size: 369 740 354 2619 Fragments arranged by size: 2619 740 369 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 634 1665 1798 2257 2466 634 Size: 1031 133 459 209 2250 Fragments arranged by size: 2250 1031 459 209 133 TatI w'GTAC_w Cuts at: 242 716 3597 242 Size: 474 2881 727 Fragments arranged by size: 2881 727 474 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 302 334 3599 3631 302 Size: 32 3265 32 753 Fragments arranged by size: 3265 753 32 32 Tth111I GACn'n_nGTC Cuts at: 1879 1879 Size: 4082 XbaI T'CTAG_A Cuts at: 1113 1113 Size: 4082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1577 1577 Size: 4082 XmaI C'CCGG_G Cuts at: 1102 1102 Size: 4082 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI