Restriction map for pF1KE0660
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       ItaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AcsI          264  AAATTT               [GA]^AATT[CT]
AluI         1155  AGCT                 AG^CT
ApoI          264  AAATTT               [GA]^AATT[CT]
AspHI         564  GAGCAC               G[AT]GC[AT]^C
AvaII         511  GGTCC                G^G[AT]CC
BamHI          63  GGATCC               G^GATCC
BglII         733  AGATCT               A^GATCT
BsaJI         175  CCTGGG               C^C[ACGT][ACGT]GG
BseRI          46  GAGGAG               GAGGAG(10/8)
BsiHKAI       564  GAGCAC               G[AT]GC[AT]^C
BsmI          310  GAATGC               GAATGC(1/-1)
BsrDI         424  CATTGC               GCAATG(2/0)
BstXI         794  CCACTTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI          716  CTCTTC               CTCTTC(1/4)
EcoT22I       674  ATGCAT               ATGCA^T
HincII        466  GTTGAC               GT[CT]^[GA]AC
HindII        466  GTTGAC               GT[CT]^[GA]AC
HphI          458  TCACC                GGTGA(8/7)
Ksp632I       716  CTCTTC               CTCTTC(1/4)
MseI          221  TTAA                 T^TAA
NdeI          336  CATATG               CA^TATG
NsiI          674  ATGCAT               ATGCA^T
NspI          676  GCATGT               [GA]CATG^[CT]
SfaNI          96  GATGC                GCATC(5/9)
SpeI          490  ACTAGT               A^CTAGT
SspI          956  AATATT               AAT^ATT
Tru9I         221  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwI           64  GATCC                GGATC(4/5)
               63  GGATC               
BbsI          142  GAAGAC               GAAGAC(2/6)
              331  GTCTTC              
BmyI          564  GAGCAC               G[AGT]GC[ACT]^C
             1049  GTGCCC              
BpuAI         142  GAAGAC               GAAGAC(2/6)
              331  GTCTTC              
BsiYI         350  CCCTTCATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              502  CCTTATAAGGG         
BslI          350  CCCTTCATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              502  CCTTATAAGGG         
BsmAI         207  GAGAC                GTCTC(1/5)
              369  GTCTC               
Bsp1286I      564  GAGCAC               G[AGT]GC[ACT]^C
             1049  GTGCCC              
BstBI        1055  TTCGAA               TT^CGAA
             1139  TTCGAA              
BstF5I        363  CATCC                GGATG(2/0)
             1012  GGATG               
BstYI          63  GGATCC               [GA]^GATC[CT]
              733  AGATCT              
Cfr13I        499  GGGCC                G^G[ACGT]CC
              511  GGTCC               
DpnII          64  GATC                 ^GATC
              734  GATC                
FokI          363  CATCC                GGATG(9/13)
             1012  GGATG               
HaeIII         33  GGCC                 GG^CC
              500  GGCC                
MaeIII        481  GTAAC                ^GT[ACGT]AC
              965  GTAAC               
MboI           64  GATC                 ^GATC
              734  GATC                
MflI           63  GGATCC               [GA]^GATC[CT]
              733  AGATCT              
NdeII          64  GATC                 ^GATC
              734  GATC                
NspV         1055  TTCGAA               TT^CGAA
             1139  TTCGAA              
Sau3AI         64  GATC                 ^GATC
              734  GATC                
Sau96I        499  GGGCC                G^G[ACGT]CC
              511  GGTCC               
SfcI           84  CTACAG               C^T[GA][CT]AG
              125  CTGTAG              
SfuI         1055  TTCGAA               TT^CGAA
             1139  TTCGAA              
TaqI         1056  TCGA                 T^CGA
             1140  TCGA                
TthHB8I      1056  TCGA                 T^CGA
             1140  TCGA                
XhoII          63  GGATCC               [GA]^GATC[CT]
              733  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

BfaI        BpmI        BsrI        BssKI       BstNI       
DdeI        EcoRII      HinfI       MaeI        MboII       
MnlI        MslI        MvaI        NlaIII      NlaIV       
PleI        ScrFI       TfiI        Tsp509I     TspRI       
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