Restriction map for pF1KB5521
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# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       BanI        BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspEI       BspMI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NarI        NciI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
StuI        SwaI        Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

AflIII        132  ACATGT               A^C[GA][CT]GT
AocI          928  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       787  AGCGCT               AGC^GCT
Asp700I       939  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         709  GAGCTC               G[AT]GC[AT]^C
AvrII         265  CCTAGG               C^CTAGG
BalI          531  TGGCCA               TGG^CCA
BamHI         834  GGATCC               G^GATCC
BbsI          552  GAAGAC               GAAGAC(2/6)
BbvCI         790  GCTGAGG              CCTCAGC(-5/-2)
BfaI          266  CTAG                 C^TAG
BlnI          265  CCTAGG               C^CTAGG
BpmI          186  CTGGAG               CTGGAG(16/14)
BpuAI         552  GAAGAC               GAAGAC(2/6)
BsaI          191  GAGACC               GGTCTC(1/5)
BsiEI        1027  CGACCG               CG[GA][CT]^CG
BsiHKAI       709  GAGCTC               G[AT]GC[AT]^C
BspDI         453  ATCGAT               AT^CGAT
BspHI         111  TCATGA               T^CATGA
BspLU11I      132  ACATGT               A^CATGT
BsrBI         934  GAGCGG               CCGCTC(-3/-3)
BsrFI         663  GCCGGC               [GA]^CCGG[CT]
BssSI         641  CTCGTG               CACGAG(-5/-1)
BstXI         585  CCAGTTACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        928  CCTCAGG              CC^T[ACGT]AGG
CfoI          788  GCGC                 GCG^C
Cfr10I        663  GCCGGC               [GA]^CCGG[CT]
ClaI          453  ATCGAT               AT^CGAT
DraII          72  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          560  CCATGG               C^C[GA][CT]GG
EaeI          531  TGGCCA               [CT]^GGCC[GA]
Eco47III      787  AGCGCT               AGC^GCT
Eco81I        928  CCTCAGG              CC^T[ACGT]AGG
EcoNI          52  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       72  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         894  GAATTC               G^AATTC
HaeII         787  AGCGCT               [GA]GCGC^[CT]
HapII         664  CCGG                 C^CGG
HhaI          788  GCGC                 GCG^C
HinP1I        788  GCGC                 G^CGC
HpaII         664  CCGG                 C^CGG
HphI          595  GGTGA                GGTGA(8/7)
MaeI          266  CTAG                 C^TAG
MluNI         531  TGGCCA               TGG^CCA
MscI          531  TGGCCA               TGG^CCA
MseI          521  TTAA                 T^TAA
MspA1I       1282  CAGCGG               C[AC]G^C[GT]G
MspI          664  CCGG                 C^CGG
NaeI          663  GCCGGC               GCC^GGC
NcoI          560  CCATGG               C^CATGG
NgoMIV        663  GCCGGC               G^CCGGC
PflMI         179  CCACAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          72  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1165  CTGCAG               CTGCA^G
RcaI          111  TCATGA               T^CATGA
SacI          709  GAGCTC               GAGCT^C
SapI          495  GAAGAGC              GCTCTTC(1/4)
SbfI         1164  CCTGCAGG             CCTGCA^GG
SfcI         1165  CTGCAG               C^T[GA][CT]AG
Sse8387I     1164  CCTGCAGG             CCTGCA^GG
SspI          335  AATATT               AAT^ATT
Tru9I         521  TTAA                 T^TAA
Van91I        179  CCACAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          939  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI         1144  GTAC                 GT^AC
             1269  GTAC                
AvaII          73  GGACC                G^G[AT]CC
              800  GGACC               
BseRI         706  GAGGAG               GAGGAG(10/8)
              793  GAGGAG              
BsmAI         191  GAGAC                GTCTC(1/5)
              444  GTCTC               
BsrDI        1085  CATTGC               GCAATG(2/0)
              205  GCAATG              
BssKI          52  CCTGG                ^CC[ACGT]GG
              468  CCTGG               
BstNI          52  CCTGG                CC^[AT]GG
              468  CCTGG               
Cac8I           6  GCGGGC               GC[ACGT]^[ACGT]GC
              663  GCCGGC              
EcoRII         52  CCTGG                ^CC[AT]GG
              468  CCTGG               
FauI            6  GCGGG                CCCGC(4/6)
              159  GCGGG               
MaeII         763  ACGT                 A^CGT
             1207  ACGT                
MvaI           52  CCTGG                CC^[AT]GG
              468  CCTGG               
NdeI          983  CATATG               CA^TATG
             1250  CATATG              
RsaI         1144  GTAC                 GT^AC
             1269  GTAC                
ScrFI          52  CCTGG                CC^[ACGT]GG
              468  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BanII       BbvI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BstF5I      BstYI       
Cfr13I      DdeI        DpnII       EarI        EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MslI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        Sau3AI      Sau96I      
SfaNI       SmlI        StyI        TaqI        TfiI        
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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