Restriction map for pF1KB5521
 (Linear) (Six-base) MAPSORT of: KIBB5521.seq  Check: 4971  from: 1  to: 1344



KIBB5521.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     738     925    1344 
   Size:        738     187     419

  Fragments arranged by size: 

                 738     419     187
 
AfeI AGC'GCT

Cuts at:      0     789    1344 
   Size:        789     555
 
AflIII A'CryG_T

Cuts at:      0     132    1344 
   Size:        132    1212
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     508     540    1344 
   Size:        508      32     804

  Fragments arranged by size: 

                 804     508      32
 
ApoI r'AATT_y

Cuts at:      0     601     894     917    1344 
   Size:        601     293      23     427

  Fragments arranged by size: 

                 601     427     293      23
 
AvrII C'CTAG_G

Cuts at:      0     265    1344 
   Size:        265    1079
 
BamHI G'GATC_C

Cuts at:      0     834    1344 
   Size:        834     510
 
BanII G_rGCy'C

Cuts at:      0     508     713    1343    1344 
   Size:        508     205     630       1

  Fragments arranged by size: 

                 630     508     205       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     559    1344 
   Size:        559     785
 
BbvCI CC'TCA_GC

Cuts at:      0     791    1344 
   Size:        791     553
 
BmgBI CAC'GTC

Cuts at:      0     764    1344 
   Size:        764     580
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1098    1344 
   Size:       1098     246
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207    1344 
   Size:        207    1137
 
Bpu10I CC'TnA_GC

Cuts at:      0     791    1344 
   Size:        791     553
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     397     522    1338    1344 
   Size:         53     344     125     816       6

  Fragments arranged by size: 

                 816     344     125      53       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     185    1344 
   Size:        185    1159
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     721     808    1344 
   Size:        721      87     536

  Fragments arranged by size: 

                 721     536      87
 
BseYI C'CCAG_C

Cuts at:      0     542     784    1308    1344 
   Size:        542     242     524      36

  Fragments arranged by size: 

                 542     524     242      36
 
BsiEI CG_ry'CG

Cuts at:      0    1030    1344 
   Size:       1030     314
 
BsiHKAI G_wGCw'C

Cuts at:      0     713    1344 
   Size:        713     631
 
Bsp1286I G_dGCh'C

Cuts at:      0     508     713    1343    1344 
   Size:        508     205     630       1

  Fragments arranged by size: 

                 630     508     205       1
 
BspHI T'CATG_A

Cuts at:      0     111    1344 
   Size:        111    1233
 
BsrBI CCG'CTC

Cuts at:      0     936    1344 
   Size:        936     408
 
BsrDI GCAATG_nn'

Cuts at:      0     212    1084    1344 
   Size:        212     872     260

  Fragments arranged by size: 

                 872     260     212
 
BsrFI r'CCGG_y

Cuts at:      0     663    1344 
   Size:        663     681
 
BssSI C'ACGA_G

Cuts at:      0     641    1344 
   Size:        641     703
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     592    1344 
   Size:        592     752
 
BstYI r'GATC_y

Cuts at:      0     381     834     953    1344 
   Size:        381     453     119     391

  Fragments arranged by size: 

                 453     391     381     119
 
Bsu36I CC'TnA_GG

Cuts at:      0     929    1344 
   Size:        929     415
 
BtgI C'CryG_G

Cuts at:      0     560    1344 
   Size:        560     784
 
BtsI GCAGTG_nn'

Cuts at:      0      13     318     887    1344 
   Size:         13     305     569     457

  Fragments arranged by size: 

                 569     457     305      13
 
ClaI AT'CG_AT

Cuts at:      0     454    1344 
   Size:        454     890
 
EaeI y'GGCC_r

Cuts at:      0     531    1344 
   Size:        531     813
 
EarI CTCTTCn'nnn_

Cuts at:      0      51     490     698     901    1344 
   Size:         51     439     208     203     443

  Fragments arranged by size: 

                 443     439     208     203      51
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     423    1344 
   Size:        423     921
 
EcoICRI GAG'CTC

Cuts at:      0     711    1344 
   Size:        711     633
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     738     925    1344 
   Size:        207     531     187     419

  Fragments arranged by size: 

                 531     419     207     187
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      56    1344 
   Size:         56    1288
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73    1344 
   Size:         73    1271
 
EcoRI G'AATT_C

Cuts at:      0     894    1344 
   Size:        894     450
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      61     516    1344 
   Size:         61     455     828

  Fragments arranged by size: 

                 828     455      61
 
HaeII r_GCGC'y

Cuts at:      0     791    1344 
   Size:        791     553
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1196    1344 
   Size:       1196     148
 
MscI TGG'CCA

Cuts at:      0     533    1344 
   Size:        533     811
 
MslI CAynn'nnrTG

Cuts at:      0     110     565     596    1107    1210    1344 
   Size:        110     455      31     511     103     134

  Fragments arranged by size: 

                 511     455     134     110     103      31
 
MspA1I CmG'CkG

Cuts at:      0    1284    1344 
   Size:       1284      60
 
NaeI GCC'GGC

Cuts at:      0     665    1344 
   Size:        665     679
 
NcoI C'CATG_G

Cuts at:      0     560    1344 
   Size:        560     784
 
NdeI CA'TA_TG

Cuts at:      0     984    1251    1344 
   Size:        984     267      93

  Fragments arranged by size: 

                 984     267      93
 
NgoMIV G'CCGG_C

Cuts at:      0     663    1344 
   Size:        663     681
 
NspI r_CATG'y

Cuts at:      0     122     136     417    1344 
   Size:        122      14     281     927

  Fragments arranged by size: 

                 927     281     122      14
 
PciI A'CATG_T

Cuts at:      0     132    1344 
   Size:        132    1212
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     185    1344 
   Size:        185    1159
 
PfoI T'CCnGG_A

Cuts at:      0      51    1344 
   Size:         51    1293
 
PpuMI rG'GwC_Cy

Cuts at:      0      73    1344 
   Size:         73    1271
 
PstI C_TGCA'G

Cuts at:      0    1169    1344 
   Size:       1169     175
 
SacI G_AGCT'C

Cuts at:      0     713    1344 
   Size:        713     631
 
SapI GCTCTTCn'nnn_

Cuts at:      0     490    1344 
   Size:        490     854
 
SbfI CC_TGCA'GG

Cuts at:      0    1169    1344 
   Size:       1169     175
 
SfcI C'TryA_G

Cuts at:      0    1165    1344 
   Size:       1165     179
 
SmlI C'TyrA_G

Cuts at:      0      68     376     501    1317    1344 
   Size:         68     308     125     816      27

  Fragments arranged by size: 

                 816     308     125      68      27
 
SspI AAT'ATT

Cuts at:      0     337    1344 
   Size:        337    1007
 
StyI C'CwwG_G

Cuts at:      0      29     265     560    1344 
   Size:         29     236     295     784

  Fragments arranged by size: 

                 784     295     236      29
 
TatI w'GTAC_w

Cuts at:      0    1143    1268    1344 
   Size:       1143     125      76

  Fragments arranged by size: 

                1143     125      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     819     851     945     977    1344 
   Size:        819      32      94      32     367

  Fragments arranged by size: 

                 819     367      94      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     943    1344 
   Size:        943     401
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AloI     ApoI    AvrII    BamHI    BanII
     BbsI    BbvCI    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrBI    BsrDI
    BsrFI    BssSI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI    PpuMI     PstI
     SacI     SapI     SbfI     SfcI     SmlI     SspI     StyI     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
    CspCI     DraI   DraIII     DrdI     EagI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com