Restriction map for pF1KB5473
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KspI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TfiI        
Tsp509I     Tth111I     XbaI        XcmI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I         55  GGTACC               G^GTACC
AcyI          196  GACGCC               G[GA]^CG[CT]C
AflIII        347  ACATGT               A^C[GA][CT]GT
Alw44I        673  GTGCAC               G^TGCAC
AocI          458  CCTCAGG              CC^T[ACGT]AGG
ApaLI         673  GTGCAC               G^TGCAC
Asp718I        55  GGTACC               G^GTACC
AvaI          641  CTCGAG               C^[CT]CG[GA]G
AvaII          15  GGTCC                G^G[AT]CC
BanI           55  GGTACC               G^G[CT][GA]CC
BbsI          726  GAAGAC               GAAGAC(2/6)
BbvCI         658  GCTGAGG              CCTCAGC(-5/-2)
BpuAI         726  GAAGAC               GAAGAC(2/6)
BsaAI         676  CACGTA               [CT]AC^GT[GA]
BsaHI         196  GACGCC               G[GA]^CG[CT]C
BsaI          693  GAGACC               GGTCTC(1/5)
BseRI         148  GAGGAG               GAGGAG(10/8)
BsgI          323  CTGCAC               GTGCAG(16/14)
BsmAI         693  GAGAC                GTCTC(1/5)
BsoBI         641  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      446  TGTACA               T^GTACA
BspLU11I      347  ACATGT               A^CATGT
BspMI         767  ACCTGC               ACCTGC(4/8)
BsrGI         446  TGTACA               T^GTACA
BsrI          650  CCAGT                ACTGG(1/-1)
BstAPI        596  GCATTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         201  CCACCTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        458  CCTCAGG              CC^T[ACGT]AGG
CfoI          375  GCGC                 GCG^C
DsaI          275  CCGTGG               C^C[GA][CT]GG
Eco81I        458  CCTCAGG              CC^T[ACGT]AGG
EcoRV          46  GATATC               GAT^ATC
FauI           19  CCCGC                CCCGC(4/6)
HapII         434  CCGG                 C^CGG
HgaI          196  GACGC                GACGC(5/10)
HhaI          375  GCGC                 GCG^C
Hin1I         196  GACGCC               G[GA]^CG[CT]C
HinP1I        375  GCGC                 G^CGC
HpaII         434  CCGG                 C^CGG
KpnI           55  GGTACC               GGTAC^C
MaeII         677  ACGT                 A^CGT
MslI          202  CACCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          434  CCGG                 C^CGG
NspI          347  ACATGT               [GA]CATG^[CT]
PaeR7I        641  CTCGAG               C^TCGAG
PflMI         270  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI         232  GCATC                GCATC(5/9)
SspBI         446  TGTACA               T^GTACA
Van91I        270  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          641  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AciI           20  CCGC                 CCGC(-3/-1)
              358  GCGG                
AfaI           56  GTAC                 GT^AC
              447  GTAC                
AlwNI         230  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              478  CAGAAACTG           
AspHI         603  GTGCTC               G[AT]GC[AT]^C
              673  GTGCAC              
BmyI          603  GTGCTC               G[AGT]GC[ACT]^C
              673  GTGCAC              
BsaJI         275  CCGTGG               C^C[ACGT][ACGT]GG
              305  CCCTGG              
BsiHKAI       603  GTGCTC               G[AT]GC[AT]^C
              673  GTGCAC              
BsiYI         204  CCTGGATGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              270  CCAGGCCGTGG         
BslI          204  CCTGGATGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              270  CCAGGCCGTGG         
BsmFI          16  GTCCC                GGGAC(10/14)
              303  GTCCC               
BsmI          361  GCATTC               GAATGC(1/-1)
              495  GCATTC              
Bsp1286I      603  GTGCTC               G[AGT]GC[ACT]^C
              673  GTGCAC              
Cac8I         155  GCCAGC               GC[ACGT]^[ACGT]GC
              293  GCTGGC              
Cfr13I         15  GGTCC                G^G[ACGT]CC
              342  GGGCC               
DdeI          459  CTCAG                C^T[ACGT]AG
              659  CTGAG               
EarI          145  GAAGAG               CTCTTC(1/4)
              190  GAAGAG              
HphI          690  GGTGA                GGTGA(8/7)
               29  TCACC               
Ksp632I       145  GAAGAG               CTCTTC(1/4)
              190  GAAGAG              
MseI          116  TTAA                 T^TAA
              712  TTAA                
MspA1I        334  CAGCTG               C[AC]G^C[GT]G
              368  CAGCTG              
NlaIII        348  CATG                 CATG^
              378  CATG                
NlaIV          15  GGTCCC               GG[ACGT]^[ACGT]CC
               55  GGTACC              
PvuII         334  CAGCTG               CAG^CTG
              368  CAGCTG              
RsaI           56  GTAC                 GT^AC
              447  GTAC                
Sau96I         15  GGTCC                G^G[ACGT]CC
              342  GGGCC               
SmlI          517  CTTGAG               C^T[CT][GA]AG
              641  CTCGAG              
Tru9I         116  TTAA                 T^TAA
              712  TTAA                
Tsp45I         28  GTCAC                ^GT[GC]AC
              533  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AluI        AlwI        BbvI        BssKI       BstF5I      
BstNI       DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      ItaI        MaeIII      MboI        MboII       
MnlI        MvaI        MwoI        NdeII       PstI        
Sau3AI      ScrFI       SfcI        TaqI        TseI        
TspRI       TthHB8I     
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