Restriction map for pF1KB5473
 (Linear) (Six-base) MAPSORT of: KIBB5473.seq  Check: 1748  from: 1  to: 783



KIBB5473.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

Acc65I G'GTAC_C

Cuts at:      0      55     783 
   Size:         55     728
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     738     783 
   Size:        209     529      45

  Fragments arranged by size: 

                 529     209      45
 
AflIII A'CryG_T

Cuts at:      0     347     783 
   Size:        347     436
 
AlwNI CAG_nnn'CTG

Cuts at:      0     235     483     783 
   Size:        235     248     300

  Fragments arranged by size: 

                 300     248     235
 
ApaLI G'TGCA_C

Cuts at:      0     673     783 
   Size:        673     110
 
AvaI C'yCGr_G

Cuts at:      0     641     783 
   Size:        641     142
 
BanI G'GyrC_C

Cuts at:      0      55     783 
   Size:         55     728
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     733     783 
   Size:        733      50
 
BbvCI CC'TCA_GC

Cuts at:      0     659     783 
   Size:        659     124
 
Bme1580I G_kGCm'C

Cuts at:      0     677     783 
   Size:        677     106
 
Bpu10I CC'TnA_GC

Cuts at:      0     659     783 
   Size:        659     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     783 
   Size:        538     245
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     687     783 
   Size:        687      96
 
BsaAI yAC'GTr

Cuts at:      0     678     783 
   Size:        678     105
 
BsaHI Gr'CG_yC

Cuts at:      0     197     783 
   Size:        197     586
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     783 
   Size:        163     620
 
BseYI C'CCAG_C

Cuts at:      0     370     783 
   Size:        370     413
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     783 
   Size:        308     475
 
BsiHKAI G_wGCw'C

Cuts at:      0     607     677     783 
   Size:        607      70     106

  Fragments arranged by size: 

                 607     106      70
 
BsmI GAATG_Cn'

Cuts at:      0     361     495     783 
   Size:        361     134     288

  Fragments arranged by size: 

                 361     288     134
 
Bsp1286I G_dGCh'C

Cuts at:      0     607     677     783 
   Size:        607      70     106

  Fragments arranged by size: 

                 607     106      70
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     776     783 
   Size:        776       7
 
BsrGI T'GTAC_A

Cuts at:      0     446     783 
   Size:        446     337
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     602     783 
   Size:        602     181
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     208     783 
   Size:        208     575
 
Bsu36I CC'TnA_GG

Cuts at:      0     459     783 
   Size:        459     324
 
BtgI C'CryG_G

Cuts at:      0     275     783 
   Size:        275     508
 
BtsI GCAGTG_nn'

Cuts at:      0     320     783 
   Size:        320     463
 
EarI CTCTTCn'nnn_

Cuts at:      0     140     185     783 
   Size:        140      45     598

  Fragments arranged by size: 

                 598     140      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     738     783 
   Size:        209     529      45

  Fragments arranged by size: 

                 529     209      45
 
EcoRV GAT'ATC

Cuts at:      0      48     783 
   Size:         48     735
 
KpnI G_GTAC'C

Cuts at:      0      59     783 
   Size:         59     724
 
MslI CAynn'nnrTG

Cuts at:      0     206     783 
   Size:        206     577
 
MspA1I CmG'CkG

Cuts at:      0     336     370     783 
   Size:        336      34     413

  Fragments arranged by size: 

                 413     336      34
 
NspI r_CATG'y

Cuts at:      0     351     783 
   Size:        351     432
 
PciI A'CATG_T

Cuts at:      0     347     783 
   Size:        347     436
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276     783 
   Size:        276     507
 
PstI C_TGCA'G

Cuts at:      0     131     231     341     783 
   Size:        131     100     110     442

  Fragments arranged by size: 

                 442     131     110     100
 
PvuII CAG'CTG

Cuts at:      0     336     370     783 
   Size:        336      34     413

  Fragments arranged by size: 

                 413     336      34
 
SfcI C'TryA_G

Cuts at:      0     127     227     337     735     783 
   Size:        127     100     110     398      48

  Fragments arranged by size: 

                 398     127     110     100      48
 
SmlI C'TyrA_G

Cuts at:      0     517     641     783 
   Size:        517     124     142

  Fragments arranged by size: 

                 517     142     124
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       3     590     783 
   Size:          3     587     193

  Fragments arranged by size: 

                 587     193       3
 
TatI w'GTAC_w

Cuts at:      0     446     783 
   Size:        446     337
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     428     460     783 
   Size:        428      32     323

  Fragments arranged by size: 

                 428     323      32
 
XhoI C'TCGA_G

Cuts at:      0     641     783 
   Size:        641     142
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI    ApaLI     AvaI     BanI     BbsI
    BbvCI Bme1580I   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrGI   BstAPI
    BstXI   Bsu36I     BtgI     BtsI     EarI  Eco57MI    EcoRV     KpnI
     MslI   MspA1I     NspI     PciI    PflMI     PstI    PvuII     SfcI
     SmlI    TaqII     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com