Restriction map for pF1KB5427
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsiEI       BsiWI       BspDI       
BspHI       BspLU11I    BsrDI       BsrFI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        137  TCCGGA               T^CCGGA
AcyI          888  GACGCC               G[GA]^CG[CT]C
AocI           68  CCTCAGG              CC^T[ACGT]AGG
BamHI         770  GGATCC               G^GATCC
BclI          986  TGATCA               T^GATCA
BglI          900  GCCCCCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         888  GACGCC               G[GA]^CG[CT]C
BsaWI         137  TCCGGA               [AT]^CCGG[AT]
BseAI         137  TCCGGA               T^CCGGA
BsgI          820  CTGCAC               GTGCAG(16/14)
BsmBI         886  GAGACG               CGTCTC(1/5)
BsmI          214  GAATGC               GAATGC(1/-1)
Bsp1407I      682  TGTACA               T^GTACA
BspEI         137  TCCGGA               T^CCGGA
BspMI         567  GCAGGT               ACCTGC(4/8)
BsrBI         993  GAGCGG               CCGCTC(-3/-3)
BsrGI         682  TGTACA               T^GTACA
BssHII        237  GCGCGC               G^CGCGC
BssSI         922  CTCGTG               CACGAG(-5/-1)
BstAPI        209  GCACGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         353  CCAAGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         770  GGATCC               [GA]^GATC[CT]
Bsu36I         68  CCTCAGG              CC^T[ACGT]AGG
DraII         356  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          893  CCATGG               C^C[GA][CT]GG
Eco81I         68  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      356  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FbaI          986  TGATCA               T^GATCA
Hin1I         888  GACGCC               G[GA]^CG[CT]C
MaeII          56  ACGT                 A^CGT
MaeIII       1072  GTGAC                ^GT[ACGT]AC
MflI          770  GGATCC               [GA]^GATC[CT]
MroI          137  TCCGGA               T^CCGGA
MseI          122  TTAA                 T^TAA
NcoI          893  CCATGG               C^CATGG
NspI          563  GCATGC               [GA]CATG^[CT]
PflMI         969  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          725  GAGTC                GAGTC(4/5)
PpuMI         356  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          150  CTGCAG               CTGCA^G
ScaI          860  AGTACT               AGT^ACT
SexAI          41  ACCTGGT              A^CC[AT]GGT
SmaI          575  CCCGGG               CCC^GGG
SphI          563  GCATGC               GCATG^C
SspBI         682  TGTACA               T^GTACA
TaqI         1056  TCGA                 T^CGA
TfiI          516  GATTC                G^A[AT]TC
Tru9I         122  TTAA                 T^TAA
Tsp45I       1072  GTGAC                ^GT[GC]AC
TthHB8I      1056  TCGA                 T^CGA
Van91I        969  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         770  GGATCC               [GA]^GATC[CT]
XmaCI         575  CCCGGG               C^CCGGG
XmaI          575  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         807  GTGCTC               G[AT]GC[AT]^C
              952  GTGCTC              
AvaI          496  CCCGAG               C^[CT]CG[GA]G
              575  CCCGGG              
BalI          362  TGGCCA               TGG^CCA
             1013  TGGCCA              
BanI          874  GGCACC               G^G[CT][GA]CC
              897  GGTGCC              
BanII         348  GGGCTC               G[GA]GC[CT]^C
             1089  GGGCTC              
BpmI          193  CTGGAG               CTGGAG(16/14)
              315  CTGGAG              
BsiHKAI       807  GTGCTC               G[AT]GC[AT]^C
              952  GTGCTC              
BsmAI         886  GAGAC                GTCTC(1/5)
               24  GTCTC               
BsoBI         496  CCCGAG               C^[CT]CG[GA]G
              575  CCCGGG              
DdeI           26  CTCAG                C^T[ACGT]AG
               69  CTCAG               
EaeI          362  TGGCCA               [CT]^GGCC[GA]
             1013  TGGCCA              
EarI           97  CTCTTC               CTCTTC(1/4)
              133  CTCTTC              
HgaI          888  GACGC                GACGC(5/10)
             1008  GACGC               
HinfI         516  GATTC                G^A[ACGT]TC
              725  GAGTC               
Ksp632I        97  CTCTTC               CTCTTC(1/4)
              133  CTCTTC              
MluNI         362  TGGCCA               TGG^CCA
             1013  TGGCCA              
MscI          362  TGGCCA               TGG^CCA
             1013  TGGCCA              
MspA1I        178  CAGCTG               C[AC]G^C[GT]G
              668  CAGCTG              
PvuII         178  CAGCTG               CAG^CTG
              668  CAGCTG              
SapI           96  GCTCTTC              GCTCTTC(1/4)
              132  GCTCTTC             
SfcI          150  CTGCAG               C^T[GA][CT]AG
              864  CTACAG              
XcmI          365  CCAAGAAAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              388  CCAATTATCGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaII       BbsI        BbvI        BcnI        
BmyI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MboI        
MboII       MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TseI        Tsp509I     
TspRI       
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