Restriction map for pF1KB5360
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbsI        BcgI        BclI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     CelII       CfoI        Cfr10I      
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         837  CGCG                 CG^CG
AlwNI         891  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanI          856  GGCACC               G^G[CT][GA]CC
BbvCI         342  CCTCAGC              CCTCAGC(-5/-2)
BciVI         468  GGATAC               GTATCC(6/5)
BcnI          157  CCGGG                CC^[GC]GG
BfaI          589  CTAG                 C^TAG
BpmI          279  CTGGAG               CTGGAG(16/14)
BsaBI         360  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         806  CTCCTC               GAGGAG(10/8)
BsiEI         475  CGATCG               CG[GA][CT]^CG
BspDI         779  ATCGAT               AT^CGAT
BstUI         837  CGCG                 CG^CG
BstXI         542  CCATGGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         874  GGATCT               [GA]^GATC[CT]
Cac8I         958  GCTTGC               GC[ACGT]^[ACGT]GC
ClaI          779  ATCGAT               AT^CGAT
DsaI          542  CCATGG               C^C[GA][CT]GG
EarI         1078  GAAGAG               CTCTTC(1/4)
EcoRI         607  GAATTC               G^AATTC
EcoT14I       542  CCATGG               C^C[AT][AT]GG
FauI          748  CCCGC                CCCGC(4/6)
HapII         157  CCGG                 C^CGG
HpaII         157  CCGG                 C^CGG
HphI          764  TCACC                GGTGA(8/7)
Ksp632I      1078  GAAGAG               CTCTTC(1/4)
MaeI          589  CTAG                 C^TAG
MaeII         530  ACGT                 A^CGT
MamI          360  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII        1078  GAAGA                GAAGA(8/7)
MflI          874  GGATCT               [GA]^GATC[CT]
MspA1I        423  CAGCTG               C[AC]G^C[GT]G
MspI          157  CCGG                 C^CGG
MvnI          837  CGCG                 CG^CG
NciI          157  CCGGG                CC^[GC]GG
NcoI          542  CCATGG               C^CATGG
NspI           47  GCATGT               [GA]CATG^[CT]
PpuMI         338  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          560  CTGCAG               CTGCA^G
PvuI          475  CGATCG               CGAT^CG
PvuII         423  CAGCTG               CAG^CTG
SapI         1078  GAAGAGC              GCTCTTC(1/4)
StyI          542  CCATGG               C^C[AT][AT]GG
Tsp45I        284  GTGAC                ^GT[GC]AC
XhoII         874  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          749  CCGC                 CCGC(-3/-1)
              823  GCGG                
AcsI          607  GAATTC               [GA]^AATT[CT]
             1027  GAATTT              
AocI           20  CCTCAGG              CC^T[ACGT]AGG
              808  CCTCAGG             
ApoI          607  GAATTC               [GA]^AATT[CT]
             1027  GAATTT              
AvaII          41  GGACC                G^G[AT]CC
              339  GGACC               
BbvI         1072  GCAGC                GCAGC(8/12)
              200  GCTGC               
BstF5I        396  CATCC                GGATG(2/0)
              844  CATCC               
BstNI         696  CCTGG                CC^[AT]GG
             1091  CCAGG               
Bsu36I         20  CCTCAGG              CC^T[ACGT]AGG
              808  CCTCAGG             
DraII         338  GGGACCT              [GA]G^G[ACGT]CC[CT]
              569  GGGGCCT             
Eco81I         20  CCTCAGG              CC^T[ACGT]AGG
              808  CCTCAGG             
EcoO109I      338  GGGACCT              [GA]G^G[ACGT]CC[CT]
              569  GGGGCCT             
EcoRII        696  CCTGG                ^CC[AT]GG
             1091  CCAGG               
Fnu4HI        200  GCTGC                GC^[ACGT]GC
             1072  GCAGC               
FokI          396  CATCC                GGATG(9/13)
              844  CATCC               
HaeIII        260  GGCC                 GG^CC
              571  GGCC                
ItaI          200  GCTGC                GC^[ACGT]GC
             1072  GCAGC               
MslI          543  CATGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              643  CATCGGTGTG          
MvaI          696  CCTGG                CC^[AT]GG
             1091  CCAGG               
PflMI         329  CCAAAATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              395  CCATCCATTGG         
TseI          200  GCTGC                G^C[AT]GC
             1072  GCAGC               
Van91I        329  CCAAAATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              395  CCATCCATTGG         
XcmI          542  CCATGGCTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              843  CCATCCTGTTCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        AlwI        BsaJI       BsiYI       BslI        
BsmFI       BsrI        BssKI       Cfr13I      DdeI        
DpnII       DraI        HinfI       MaeIII      MboI        
MnlI        MseI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       Tsp509I     
TspRI       TthHB8I     
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