Restriction map for pF1KB8735
 (Linear) (Six-base) MAPSORT of: KIBB8735.seq  Check: 7786  from: 1  to: 822



KIBB8735.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     653     822 
   Size:        653     169
 
AflIII A'CryG_T

Cuts at:      0      23     437     822 
   Size:         23     414     385

  Fragments arranged by size: 

                 414     385      23
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     115     822 
   Size:        115     707
 
AlwNI CAG_nnn'CTG

Cuts at:      0     150     822 
   Size:        150     672
 
ApaI G_GGCC'C

Cuts at:      0     317     822 
   Size:        317     505
 
ApaLI G'TGCA_C

Cuts at:      0     286     292     822 
   Size:        286       6     530

  Fragments arranged by size: 

                 530     286       6
 
AvaI C'yCGr_G

Cuts at:      0     181     207     822 
   Size:        181      26     615

  Fragments arranged by size: 

                 615     181      26
 
BanI G'GyrC_C

Cuts at:      0      16     119     216     255     393     822 
   Size:         16     103      97      39     138     429

  Fragments arranged by size: 

                 429     138     103      97      39      16
 
BanII G_rGCy'C

Cuts at:      0     173     205     317     743     758     822 
   Size:        173      32     112     426      15      64

  Fragments arranged by size: 

                 426     173     112      64      32      15
 
BbeI G_GCGC'C

Cuts at:      0     123     220     259     822 
   Size:        123      97      39     563

  Fragments arranged by size: 

                 563     123      97      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     708     822 
   Size:        708     114
 
BglI GCCn_nnn'nGGC

Cuts at:      0     209     402     822 
   Size:        209     193     420

  Fragments arranged by size: 

                 420     209     193
 
BlpI GC'TnA_GC

Cuts at:      0     742     822 
   Size:        742      80
 
Bme1580I G_kGCm'C

Cuts at:      0      19     290     296     317     822 
   Size:         19     271       6      21     505

  Fragments arranged by size: 

                 505     271      21      19       6
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     271     429     781     822 
   Size:        187      84     158     352      41

  Fragments arranged by size: 

                 352     187     158      84      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     792     822 
   Size:         70     722      30

  Fragments arranged by size: 

                 722      70      30
 
BsaAI yAC'GTr

Cuts at:      0      35     285     291     822 
   Size:         35     250       6     531

  Fragments arranged by size: 

                 531     250      35       6
 
BsaHI Gr'CG_yC

Cuts at:      0     120     217     256     777     822 
   Size:        120      97      39     521      45

  Fragments arranged by size: 

                 521     120      97      45      39
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     822 
   Size:        142     680
 
BseYI C'CCAG_C

Cuts at:      0     595     649     678     721     822 
   Size:        595      54      29      43     101

  Fragments arranged by size: 

                 595     101      54      43      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     625     822 
   Size:        289     336     197

  Fragments arranged by size: 

                 336     289     197
 
BsiEI CG_ry'CG

Cuts at:      0     702     822 
   Size:        702     120
 
BsiHKAI G_wGCw'C

Cuts at:      0     290     296     509     743     767     822 
   Size:        290       6     213     234      24      55

  Fragments arranged by size: 

                 290     234     213      55      24       6
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     173     205     290     296     317     509     743 
   Size:         19     154      32      85       6      21     192     234

Cuts at:    743     758     767     822 
   Size:         15       9      55

  Fragments arranged by size: 

                 234     192     154      85      55      32      21      19
                  15       9       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     158     653     822 
   Size:        158     495     169

  Fragments arranged by size: 

                 495     169     158
 
BsrBI CCG'CTC

Cuts at:      0     609     822 
   Size:        609     213
 
BsrFI r'CCGG_y

Cuts at:      0      43     258     783     822 
   Size:         43     215     525      39

  Fragments arranged by size: 

                 525     215      43      39
 
BssHII G'CGCG_C

Cuts at:      0     153     822 
   Size:        153     669
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     150     300     822 
   Size:        150     150     522

  Fragments arranged by size: 

                 522     150     150
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     407     822 
   Size:        407     415
 
BtgI C'CryG_G

Cuts at:      0     309     400     702     822 
   Size:        309      91     302     120

  Fragments arranged by size: 

                 309     302     120      91
 
EaeI y'GGCC_r

Cuts at:      0     194     705     822 
   Size:        194     511     117

  Fragments arranged by size: 

                 511     194     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     455     616     822 
   Size:        455     161     206

  Fragments arranged by size: 

                 455     206     161
 
EcoICRI GAG'CTC

Cuts at:      0     741     822 
   Size:        741      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     271     429     781     822 
   Size:        187      84     158     352      41

  Fragments arranged by size: 

                 352     187     158      84      41
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     627     822 
   Size:        627     195
 
HaeII r_GCGC'y

Cuts at:      0     123     220     259     640     656     822 
   Size:        123      97      39     381      16     166

  Fragments arranged by size: 

                 381     166     123      97      39      16
 
KasI G'GCGC_C

Cuts at:      0     119     216     255     822 
   Size:        119      97      39     567

  Fragments arranged by size: 

                 567     119      97      39
 
MspA1I CmG'CkG

Cuts at:      0     300     725     822 
   Size:        300     425      97

  Fragments arranged by size: 

                 425     300      97
 
NaeI GCC'GGC

Cuts at:      0      45     260     822 
   Size:         45     215     562

  Fragments arranged by size: 

                 562     215      45
 
NarI GG'CG_CC

Cuts at:      0     120     217     256     822 
   Size:        120      97      39     566

  Fragments arranged by size: 

                 566     120      97      39
 
NgoMIV G'CCGG_C

Cuts at:      0      43     258     822 
   Size:         43     215     564

  Fragments arranged by size: 

                 564     215      43
 
NspI r_CATG'y

Cuts at:      0     441     822 
   Size:        441     381
 
PasI CC'CwG_GG

Cuts at:      0      12     822 
   Size:         12     810
 
PciI A'CATG_T

Cuts at:      0     437     822 
   Size:        437     385
 
PfoI T'CCnGG_A

Cuts at:      0     614     822 
   Size:        614     208
 
PmlI CAC'GTG

Cuts at:      0      35     285     291     822 
   Size:         35     250       6     531

  Fragments arranged by size: 

                 531     250      35       6
 
PspOMI G'GGCC_C

Cuts at:      0     313     822 
   Size:        313     509
 
PspXI vC'TCGA_Gb

Cuts at:      0     181     822 
   Size:        181     641
 
PstI C_TGCA'G

Cuts at:      0      94     695     822 
   Size:         94     601     127

  Fragments arranged by size: 

                 601     127      94
 
PvuII CAG'CTG

Cuts at:      0     725     822 
   Size:        725      97
 
SacI G_AGCT'C

Cuts at:      0     743     822 
   Size:        743      79
 
SapI GCTCTTCn'nnn_

Cuts at:      0     616     822 
   Size:        616     206
 
SbfI CC_TGCA'GG

Cuts at:      0     695     822 
   Size:        695     127
 
SfcI C'TryA_G

Cuts at:      0      90     495     691     812     822 
   Size:         90     405     196     121      10

  Fragments arranged by size: 

                 405     196     121      90      10
 
SfoI GGC'GCC

Cuts at:      0     121     218     257     822 
   Size:        121      97      39     565

  Fragments arranged by size: 

                 565     121      97      39
 
SmaI CCC'GGG

Cuts at:      0     209     822 
   Size:        209     613
 
SmlI C'TyrA_G

Cuts at:      0     181     822 
   Size:        181     641
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     356     745     822 
   Size:        356     389      77

  Fragments arranged by size: 

                 389     356      77
 
XhoI C'TCGA_G

Cuts at:      0     181     822 
   Size:        181     641
 
XmaI C'CCGG_G

Cuts at:      0     207     822 
   Size:        207     615
 

 Enzymes that do cut:

     AarI   AflIII     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbeI     BbsI     BglI     BlpI Bme1580I     BpmI     BsaI
    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstXI     BtgI     EaeI
     EarI  EcoICRI  Eco57MI     FalI    HaeII     KasI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI     PciI     PfoI     PmlI   PspOMI
    PspXI     PstI    PvuII     SacI     SapI     SbfI     SfcI     SfoI
     SmaI     SmlI    TaqII     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaWI
    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com