Restriction map for pF1KB5863
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbvCI       
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FspI        HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        MamI        
MluI        MroI        MseI        MslI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsaI        RsrII       SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          259  GTCGAC               GT^[AC][GT]AC
ApaI         1210  GGGCCC               GGGCC^C
AvaI         1171  CTCGGG               C^[CT]CG[GA]G
BalI           32  TGGCCA               TGG^CCA
BbrPI         327  CACGTG               CAC^GTG
BcgI          326  GCACGTGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          960  CCCGG                CC^[GC]GG
BfaI          454  CTAG                 C^TAG
BglII         185  AGATCT               A^GATCT
BsaAI         327  CACGTG               [CT]AC^GT[GA]
BsaI         1006  GAGACC               GGTCTC(1/5)
BsoBI        1171  CTCGGG               C^[CT]CG[GA]G
BspHI        1127  TCATGA               T^CATGA
BsrBI         166  GAGCGG               CCGCTC(-3/-3)
BssSI        1012  CTCGTG               CACGAG(-5/-1)
BstEII        265  GGTGACC              G^GT[ACGT]ACC
BstPI         265  GGTGACC              G^GT[ACGT]ACC
DraIII        100  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1368  GAAGAG               CTCTTC(1/4)
EcoNI         453  CCTAGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       265  GGTGACC              G^GT[ACGT]ACC
FseI          996  GGCCGGCC             GGCCGG^CC
HincII        259  GTCGAC               GT[CT]^[GA]AC
HindII        259  GTCGAC               GT[CT]^[GA]AC
Ksp632I      1368  GAAGAG               CTCTTC(1/4)
KspI          628  CCGCGG               CCGC^GG
MaeI          454  CTAG                 C^TAG
MfeI          414  CAATTG               C^AATTG
MluNI          32  TGGCCA               TGG^CCA
MscI           32  TGGCCA               TGG^CCA
MunI          414  CAATTG               C^AATTG
NaeI          997  GCCGGC               GCC^GGC
NciI          960  CCCGG                CC^[GC]GG
NgoMIV        997  GCCGGC               G^CCGGC
NspI         1151  ACATGC               [GA]CATG^[CT]
PmaCI         327  CACGTG               CAC^GTG
PmlI          327  CACGTG               CAC^GTG
PstI          517  CTGCAG               CTGCA^G
PvuII         354  CAGCTG               CAG^CTG
RcaI         1127  TCATGA               T^CATGA
SacII         628  CCGCGG               CCGC^GG
SalI          259  GTCGAC               G^TCGAC
SapI         1368  GAAGAGC              GCTCTTC(1/4)
TfiI         1092  GATTC                G^A[AT]TC
XcmI          128  CCAACTCCTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI           44  GAATTT               [GA]^AATT[CT]
              212  AAATTT              
ApoI           44  GAATTT               [GA]^AATT[CT]
              212  AAATTT              
BamHI         113  GGATCC               G^GATCC
             1331  GGATCC              
BanI         1234  GGCACC               G^G[CT][GA]CC
             1377  GGCACC              
BglI          365  GCCGCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              709  GCCTGCGGGGC         
BspMI         806  ACCTGC               ACCTGC(4/8)
              675  GCAGGT              
BsrFI         997  GCCGGC               [GA]^CCGG[CT]
             1178  ACCGGC              
Cfr10I        997  GCCGGC               [GA]^CCGG[CT]
             1178  ACCGGC              
DdeI           72  CTCAG                C^T[ACGT]AG
             1145  CTGAG               
DraII         553  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1209  GGGGCCC             
EaeI           32  TGGCCA               [CT]^GGCC[GA]
              999  CGGCCA              
EcoO109I      553  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1209  GGGGCCC             
HaeII         473  GGCGCT               [GA]GCGC^[CT]
              812  GGCGCT              
NcoI         1214  CCATGG               C^CATGG
             1335  CCATGG              
SacI          145  GAGCTC               GAGCT^C
              985  GAGCTC              
SexAI         101  ACCAGGT              A^CC[AT]GGT
             1237  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AspHI       AvaII       BanII       BbsI        BbvI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DpnII       DsaI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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