Restriction map for pF1KE6728
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BcnI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HindIII     HpaII       KasI        
KpnI        KspI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       StyI        SwaI        Tth111I     XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   28 enzymes) #
###################################

AflII        1533  CTTAAG               C^TTAAG
BbsI         1156  GAAGAC               GAAGAC(2/6)
BbvCI          82  GCTGAGG              CCTCAGC(-5/-2)
BclI         1410  TGATCA               T^GATCA
BfrI         1533  CTTAAG               C^TTAAG
BpmI          219  CTGGAG               CTGGAG(16/14)
BpuAI        1156  GAAGAC               GAAGAC(2/6)
BsaAI        2367  CACGTA               [CT]AC^GT[GA]
BsgI          272  GTGCAG               GTGCAG(16/14)
BsmBI         222  GAGACG               CGTCTC(1/5)
BspHI        2642  TCATGA               T^CATGA
BspLU11I     1977  ACATGT               A^CATGT
EarI         2561  CTCTTC               CTCTTC(1/4)
EcoNI        2656  CCTGAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1732  ATGCAT               ATGCA^T
FbaI         1410  TGATCA               T^GATCA
HgaI         1478  GACGC                GACGC(5/10)
Ksp632I      2561  CTCTTC               CTCTTC(1/4)
MspA1I         80  CAGCTG               C[AC]G^C[GT]G
NdeI         1790  CATATG               CA^TATG
NsiI         1732  ATGCAT               ATGCA^T
PvuII          80  CAGCTG               CAG^CTG
RcaI         2642  TCATGA               T^CATGA
SacI         1387  GAGCTC               GAGCT^C
SpeI         2407  ACTAGT               A^CTAGT
StuI         2628  AGGCCT               AGG^CCT
XbaI         1284  TCTAGA               T^CTAGA
XcmI          149  CCAACCACACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AflIII        225  ACGTGT               A^C[GA][CT]GT
             1977  ACATGT              
AlwNI         742  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1696  CAGTTACTG           
BanII         277  GGGCTC               G[GA]GC[CT]^C
             1387  GAGCTC              
BsaBI         844  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1405  GATTTTGATC          
BsmFI         362  GTCCC                GGGAC(10/14)
             2862  GTCCC               
BssKI         115  CCAGG                ^CC[ACGT]GG
              414  CCAGG               
BstNI         115  CCAGG                CC^[AT]GG
              414  CCAGG               
BstXI         464  CCAAGACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1313  CCACAAATGTGG        
BstYI           3  GGATCT               [GA]^GATC[CT]
             2652  AGATCC              
DraII        1308  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2774  AGGACCC             
EcoO109I     1308  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2774  AGGACCC             
EcoRII        115  CCAGG                ^CC[AT]GG
              414  CCAGG               
MamI          844  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1405  GATTTTGATC          
MflI            3  GGATCT               [GA]^GATC[CT]
             2652  AGATCC              
MslI         1314  CACAAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1725  CACAAAGATG          
MvaI          115  CCAGG                CC^[AT]GG
              414  CCAGG               
PpuMI        1308  AGGACCC              [GA]G^G[AT]CC[CT]
             2774  AGGACCC             
PstI         1702  CTGCAG               CTGCA^G
             2797  CTGCAG              
ScrFI         115  CCAGG                CC^[ACGT]GG
              414  CCAGG               
XhoII           3  GGATCT               [GA]^GATC[CT]
             2652  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AspHI       AvaII       BbvI        BciVI       
BfaI        BmyI        BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmI        Bsp1286I    BsrDI       
BsrI        BstAPI      BstF5I      Cac8I       Cfr13I      
DdeI        DpnII       DraI        Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HpaI        
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        RsaI        Sau3AI      Sau96I      SfaNI       
SfcI        SmlI        SspI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XmnI        
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