Restriction map for pF1KE6728
 (Circular) (Six-base) MAPSORT of: pF1KE6728.seq  Check: 6511  from: 1  to: 6038



pF1KE6728  6038 bp

 With 182 enzymes: SgfI * 

                        October 20, 2017 17:04  ..

AccI GT'mk_AC

Cuts at:   3076    3076 
   Size:       6038
 
Acc65I G'GTAC_C

Cuts at:   3054    3054 
   Size:       6038
 
AclI AA'CG_TT

Cuts at:   5878    5878 
   Size:       6038
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    124    1259    1355    2899    3863    4295    5042    5310     124 
   Size:       1135      96    1544     964     432     747     268     852

  Fragments arranged by size: 

                1544    1135     964     852     747     432     268      96
            
 
AflII C'TTAA_G

Cuts at:   1641    1641 
   Size:       6038
 
AflIII A'CryG_T

Cuts at:    333    2085    3390    4494    5471     333 
   Size:       1752    1305    1104     977     900

  Fragments arranged by size: 

                1752    1305    1104     977     900
 
AgeI A'CCGG_T

Cuts at:   4426    4426 
   Size:       6038
 
AleI CACnn'nnGTG

Cuts at:   1426    1426 
   Size:       6038
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1771    1803    2948    2980    1771 
   Size:         32    1145      32    4829

  Fragments arranged by size: 

                4829    1145      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    855    1809    3343    4910     855 
   Size:        954    1534    1567    1983

  Fragments arranged by size: 

                1983    1567    1534     954
 
ApaLI G'TGCA_C

Cuts at:   4808    4808 
   Size:       6038
 
ApoI r'AATT_y

Cuts at:    635     997    1119    1627    1747    2266    2554    2704    3042 
   Size:        362     122     508     120     519     288     150     338

Cuts at:   3042    5391    5647     635 
   Size:       2349     256    1026

  Fragments arranged by size: 

                2349    1026     519     508     362     338     288     256
                 150     122     120
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6038
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6038
 
AvaI C'yCGr_G

Cuts at:   3058    3058 
   Size:       6038
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    161     194     161 
   Size:         33    6005
 
BamHI G'GATC_C

Cuts at:   3063    3063 
   Size:       6038
 
BanI G'GyrC_C

Cuts at:   3054    3468    3716    3751    3054 
   Size:        414     248      35    5341

  Fragments arranged by size: 

                5341     414     248      35
 
BanII G_rGCy'C

Cuts at:    389    1499    3052    4082     389 
   Size:       1110    1553    1030    2345

  Fragments arranged by size: 

                2345    1553    1110    1030
 
BbeI G_GCGC'C

Cuts at:   3472    3720    3472 
   Size:        248    5790
 
BbsI GAAGACnn'nnnn_

Cuts at:   1271    1271 
   Size:       6038
 
BbvCI CC'TCA_GC

Cuts at:    191     191 
   Size:       6038
 
BciVI GTATCCnnnnn_n'

Cuts at:    162    2468    2982    3936    4697    6038     162 
   Size:       2306     514     954     761    1341     162

  Fragments arranged by size: 

                2306    1341     954     761     514     162
 
BclI T'GATC_A

Cuts at:   1518    3558    1518 
   Size:       2040    3998
 
BfrBI ATG'CAT

Cuts at:   1842    1842 
   Size:       6038
 
BglII A'GATC_T

Cuts at:   3553    5590    3553 
   Size:       2037    4001
 
BlpI GC'TnA_GC

Cuts at:   3150    3150 
   Size:       6038
 
Bme1580I G_kGCm'C

Cuts at:   3663    3756    4812    3663 
   Size:         93    1056    4889

  Fragments arranged by size: 

                4889    1056      93
 
BmgBI CAC'GTC

Cuts at:    334     334 
   Size:       6038
 
BmrI ACTGGGnnnn_n'

Cuts at:   2041    3250    3331    3666    5810    2041 
   Size:       1209      81     335    2144    2269

  Fragments arranged by size: 

                2269    2144    1209     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4405    4405 
   Size:       6038
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    348     348 
   Size:       6038
 
Bpu10I CC'TnA_GC

Cuts at:    191     191 
   Size:       6038
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2338    2827    2914    3214    4093    4585    4883    2338 
   Size:        489      87     300     879     492     298    3493

  Fragments arranged by size: 

                3493     879     492     489     300     298      87
 
BsaI GGTCTCn'nnnn_

Cuts at:   5742    5742 
   Size:       6038
 
BsaAI yAC'GTr

Cuts at:   2477    3391    2477 
   Size:        914    5124
 
BsaBI GATnn'nnATC

Cuts at:      6     956    1517    3552    5589       6 
   Size:        950     561    2035    2037     455

  Fragments arranged by size: 

                2037    2035     950     561     455
 
BsaHI Gr'CG_yC

Cuts at:   3469    3717    4419    5915    3469 
   Size:        248     702    1496    3592

  Fragments arranged by size: 

                3592    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   3227    3748    4426    4700    4847    5258    3227 
   Size:        521     678     274     147     411    4007

  Fragments arranged by size: 

                4007     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1365    1395    1365 
   Size:         30    6008
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    208     373     376     965    1313     208 
   Size:        165       3     589     348    4933

  Fragments arranged by size: 

                4933     589     348     165       3
 
BseYI C'CCAG_C

Cuts at:   2627    3005    3463    4221    4798    5294    2627 
   Size:        378     458     758     577     496    3371

  Fragments arranged by size: 

                3371     758     577     496     458     378
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    401     401 
   Size:       6038
 
BsiEI CG_ry'CG

Cuts at:    104    3580    3626    4834     104 
   Size:       3476      46    1208    1308

  Fragments arranged by size: 

                3476    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    443    1499    2354    3052    3830    4020    4812     443 
   Size:       1056     855     698     778     190     792    1669

  Fragments arranged by size: 

                1669    1056     855     792     778     698     190
 
BsmI GAATG_Cn'

Cuts at:    865    1171    1446     865 
   Size:        306     275    5457

  Fragments arranged by size: 

                5457     306     275
 
BsmBI CGTCTCn'nnnn_

Cuts at:    324     324 
   Size:       6038
 
Bsp1286I G_dGCh'C

Cuts at:    389     443    1499    2354    3052    3663    3756    3830    4020 
   Size:         54    1056     855     698     611      93      74     190

Cuts at:   4020    4082    4812     389 
   Size:         62     730    1615

  Fragments arranged by size: 

                1615    1056     855     730     698     611     190      93
                  74      62      54
 
BspEI T'CCGG_A

Cuts at:   5258    5258 
   Size:       6038
 
BspHI T'CATG_A

Cuts at:   2750    5214    2750 
   Size:       2464    3574
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3088    3302    3604    3985    3088 
   Size:        214     302     381    5141

  Fragments arranged by size: 

                5141     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4330    4384    5868    6033      50 
   Size:       4280      54    1484     165      55

  Fragments arranged by size: 

                4280    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    443     711    1579    3950     443 
   Size:        268     868    2371    2531

  Fragments arranged by size: 

                2531    2371     868     268
 
BsrFI r'CCGG_y

Cuts at:   4036    4217    4426    4036 
   Size:        181     209    5648

  Fragments arranged by size: 

                5648     209     181
 
BssSI C'ACGA_G

Cuts at:   4309    4667    4309 
   Size:        358    5680
 
BstAPI GCAn_nnn'nTGC

Cuts at:    346     454    1848    2946    3415     346 
   Size:        108    1394    1098     469    2969

  Fragments arranged by size: 

                2969    1394    1098     469     108
 
BstBI TT'CG_AA

Cuts at:   4399    4399 
   Size:       6038
 
BstXI CCAn_nnnn'nTGG

Cuts at:    579    1428     579 
   Size:        849    5189
 
BstYI r'GATC_y

Cuts at:      1     111    2760    3063    3530    3553    3888    4134    5146 
   Size:        110    2649     303     467      23     335     246    1012

Cuts at:   5146    5232    5244    5590       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                2649    1012     467     449     346     335     303     246
                 110      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       6038
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3988    4169    4332    3988 
   Size:        181     163    5694

  Fragments arranged by size: 

                5694     181     163
 
BtsI GCAGTG_nn'

Cuts at:    214     443     739    2902    3281     214 
   Size:        229     296    2163     379    2971

  Fragments arranged by size: 

                2971    2163     379     296     229
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   2463    2498    2463 
   Size:         35    6003
 
DraI TTT'AAA

Cuts at:    306    1530    2318    3037     306 
   Size:       1224     788     719    3307

  Fragments arranged by size: 

                3307    1224     788     719
 
DrdI GACnn_nn'nnGTC

Cuts at:   3744    4602    3744 
   Size:        858    5180
 
EaeI y'GGCC_r

Cuts at:   3623    3797    4188    4215    5560    3623 
   Size:        174     391      27    1345    4101

  Fragments arranged by size: 

                4101    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3623    3623 
   Size:       6038
 
EarI CTCTTCn'nnn_

Cuts at:   2675    4061    4271    2675 
   Size:       1386     210    4442

  Fragments arranged by size: 

                4442    1386     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4246    4556    4702    5848    4246 
   Size:        310     146    1146    4436

  Fragments arranged by size: 

                4436    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1497    3050    1497 
   Size:       1553    4485
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    124     348    1259    1355    2899    3863    4295    5042    5310 
   Size:        224     911      96    1544     964     432     747     268

Cuts at:   5310     124 
   Size:        852

  Fragments arranged by size: 

                1544     964     911     852     747     432     268     224
                  96
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2768    2768 
   Size:       6038
 
EcoO109I rG'GnC_Cy

Cuts at:   1417    2883    3177    1417 
   Size:       1466     294    4278

  Fragments arranged by size: 

                4278    1466     294
 
EcoRI G'AATT_C

Cuts at:   3042    3042 
   Size:       6038
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1656    4057    1656 
   Size:       2401    3637
 
FspI TGC'GCA

Cuts at:   3819    3819 
   Size:       6038
 
HaeII r_GCGC'y

Cuts at:   3472    3720    4742    5721    3472 
   Size:        248    1022     979    3789

  Fragments arranged by size: 

                3789    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    411     443    1043    1075    1365    1397    2745    2777    2787 
   Size:         32     600      32     290      32    1348      32      10

Cuts at:   2787    2819    3880    3910    3912    3942     411 
   Size:         32    1061      30       2      30    2507

  Fragments arranged by size: 

                2507    1348    1061     600     290      32      32      32
                  32      32      30      30      10       2
 
HincII GTy'rAC

Cuts at:    149     770    1730    1881    3077     149 
   Size:        621     960     151    1196    3110

  Fragments arranged by size: 

                3110    1196     960     621     151
 
HpaI GTT'AAC

Cuts at:    149     770    1730     149 
   Size:        621     960    4457

  Fragments arranged by size: 

                4457     960     621
 
KasI G'GCGC_C

Cuts at:   3468    3716    3468 
   Size:        248    5790
 
KpnI G_GTAC'C

Cuts at:   3058    3058 
   Size:       6038
 
MluI A'CGCG_T

Cuts at:   5471    5471 
   Size:       6038
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1458    4709    4893    1458 
   Size:       3251     184    2603

  Fragments arranged by size: 

                3251    2603     184
 
MscI TGG'CCA

Cuts at:   3799    3799 
   Size:       6038
 
MslI CAynn'nnrTG

Cuts at:   1426    1837    4027    4154    1426 
   Size:        411    2190     127    3310

  Fragments arranged by size: 

                3310    2190     411     127
 
MspA1I CmG'CkG

Cuts at:    190    3149    3463    3823    4836    5081     190 
   Size:       2959     314     360    1013     245    1147

  Fragments arranged by size: 

                2959    1147    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   4219    4219 
   Size:       6038
 
NarI GG'CG_CC

Cuts at:   3469    3717    3469 
   Size:        248    5790
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       6038
 
NdeI CA'TA_TG

Cuts at:   1899    1899 
   Size:       6038
 
NgoMIV G'CCGG_C

Cuts at:   4217    4217 
   Size:       6038
 
NsiI A_TGCA'T

Cuts at:   1844    1844 
   Size:       6038
 
NspI r_CATG'y

Cuts at:   1846    1855    1887    2089    3091    3591    4498    1846 
   Size:          9      32     202    1002     500     907    3386

  Fragments arranged by size: 

                3386    1002     907     500     202      32       9
 
PciI A'CATG_T

Cuts at:   2085    4494    2085 
   Size:       2409    3629
 
PflMI CCAn_nnn'nTGG

Cuts at:    267     479    2580     267 
   Size:        212    2101    3725

  Fragments arranged by size: 

                3725    2101     212
 
PfoI T'CCnGG_A

Cuts at:    521     521 
   Size:       6038
 
PmeI GTTT'AAAC

Cuts at:   3037    3037 
   Size:       6038
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5177    5209    5177 
   Size:         32    6006
 
PpuMI rG'GwC_Cy

Cuts at:   1417    2883    1417 
   Size:       1466    4572
 
PsiI TTA'TAA

Cuts at:    641    1106    2719    2780     641 
   Size:        465    1613      61    3899

  Fragments arranged by size: 

                3899    1613     465      61
 
PstI C_TGCA'G

Cuts at:   1814    2909    3085    3770    1814 
   Size:       1095     176     685    4082

  Fragments arranged by size: 

                4082    1095     685     176
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6038
 
PvuII CAG'CTG

Cuts at:    190    3463    3823     190 
   Size:       3273     360    2405

  Fragments arranged by size: 

                3273    2405     360
 
RsrII CG'GwC_CG

Cuts at:   4233    4233 
   Size:       6038
 
SacI G_AGCT'C

Cuts at:   1499    3052    1499 
   Size:       1553    4485
 
SalI G'TCGA_C

Cuts at:   3075    3075 
   Size:       6038
 
SapI GCTCTTCn'nnn_

Cuts at:   4061    4271    4061 
   Size:        210    5828
 
SbfI CC_TGCA'GG

Cuts at:   3085    3085 
   Size:       6038
 
ScaI AGT'ACT

Cuts at:   5555    5555 
   Size:       6038
 
SfcI C'TryA_G

Cuts at:     33    1810    2330    2462    2905    3010    3081    3766    4759 
   Size:       1777     520     132     443     105      71     685     993

Cuts at:   4759    4950      33 
   Size:        191    1121

  Fragments arranged by size: 

                1777    1121     993     685     520     443     191     132
                 105      71
 
SfoI GGC'GCC

Cuts at:   3470    3718    3470 
   Size:        248    5790
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6038
 
SmaI CCC'GGG

Cuts at:   3060    3060 
   Size:       6038
 
SmlI C'TyrA_G

Cuts at:   1641    2353    2806    2929    3193    4108    4600    4862    1641 
   Size:        712     453     123     264     915     492     262    2817

  Fragments arranged by size: 

                2817     915     712     492     453     264     262     123
            
 
SpeI A'CTAG_T

Cuts at:     67    2515      67 
   Size:       2448    3590
 
SphI G_CATG'C

Cuts at:   3091    3591    3091 
   Size:        500    5538
 
SspI AAT'ATT

Cuts at:    249     833    1921     249 
   Size:        584    1088    4366

  Fragments arranged by size: 

                4366    1088     584
 
StuI AGG'CCT

Cuts at:   2738    2738 
   Size:       6038
 
StyI C'CwwG_G

Cuts at:    107    3172    3526     107 
   Size:       3065     354    2619

  Fragments arranged by size: 

                3065    2619     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1597    2856    3019    3621    3754    4213    4422    1597 
   Size:       1259     163     602     133     459     209    3213

  Fragments arranged by size: 

                3213    1259     602     459     209     163     133
 
TatI w'GTAC_w

Cuts at:    294    1386    2062    5553     294 
   Size:       1092     676    3491     779

  Fragments arranged by size: 

                3491    1092     779     676
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1929    1961    5555    5587    1929 
   Size:         32    3594      32    2380

  Fragments arranged by size: 

                3594    2380      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3835    3835 
   Size:       6038
 
XbaI T'CTAG_A

Cuts at:   1392    3069    1392 
   Size:       1677    4361
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    264    3533     264 
   Size:       3269    2769
 
XmaI C'CCGG_G

Cuts at:   3058    3058 
   Size:       6038
 
XmnI GAAnn'nnTTC

Cuts at:    250     491    2646     250 
   Size:        241    2155    3642

  Fragments arranged by size: 

                3642    2155     241
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AleI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AlfI     ApaI     AscI    AvrII
     BcgI     BglI     BmtI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI   DraIII    EcoRV     FseI    FspAI  HindIII     MfeI
     NheI     NotI     NruI     PacI     PasI     PmlI    PshAI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SwaI     XhoI     ZraI
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