Restriction map for pF1KE6728
 (Linear) (Six-base) MAPSORT of: KIEE6728.seq  Check: 1490  from: 1  to: 2925



KIEE6728.orf

 With 182 enzymes: SgfI * 

                        October 20, 2017 17:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1151    1247    2791    2925 
   Size:         16    1135      96    1544     134

  Fragments arranged by size: 

                1544    1135     134      96      16
 
AflII C'TTAA_G

Cuts at:      0    1533    2925 
   Size:       1533    1392
 
AflIII A'CryG_T

Cuts at:      0     225    1977    2925 
   Size:        225    1752     948

  Fragments arranged by size: 

                1752     948     225
 
AleI CACnn'nnGTG

Cuts at:      0    1318    2925 
   Size:       1318    1607
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1663    1695    2840    2872    2925 
   Size:       1663      32    1145      32      53

  Fragments arranged by size: 

                1663    1145      53      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     747    1701    2925 
   Size:        747     954    1224

  Fragments arranged by size: 

                1224     954     747
 
ApoI r'AATT_y

Cuts at:      0     527     889    1011    1519    1639    2158    2446    2596 
   Size:        527     362     122     508     120     519     288     150

Cuts at:   2596    2925 
   Size:        329

  Fragments arranged by size: 

                 527     519     508     362     329     288     150     122
                 120
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      53      86    2925 
   Size:         53      33    2839

  Fragments arranged by size: 

                2839      53      33
 
BanII G_rGCy'C

Cuts at:      0     281    1391    2925 
   Size:        281    1110    1534

  Fragments arranged by size: 

                1534    1110     281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1163    2925 
   Size:       1163    1762
 
BbvCI CC'TCA_GC

Cuts at:      0      83    2925 
   Size:         83    2842
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      54    2360    2874    2925 
   Size:         54    2306     514      51

  Fragments arranged by size: 

                2306     514      54      51
 
BclI T'GATC_A

Cuts at:      0    1410    2925 
   Size:       1410    1515
 
BfrBI ATG'CAT

Cuts at:      0    1734    2925 
   Size:       1734    1191
 
BmgBI CAC'GTC

Cuts at:      0     226    2925 
   Size:        226    2699
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1933    2925 
   Size:       1933     992
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    2925 
   Size:        240    2685
 
Bpu10I CC'TnA_GC

Cuts at:      0      83    2925 
   Size:         83    2842
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2230    2719    2806    2925 
   Size:       2230     489      87     119

  Fragments arranged by size: 

                2230     489     119      87
 
BsaAI yAC'GTr

Cuts at:      0    2369    2925 
   Size:       2369     556
 
BsaBI GATnn'nnATC

Cuts at:      0     848    1409    2925 
   Size:        848     561    1516

  Fragments arranged by size: 

                1516     848     561
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1257    1287    2925 
   Size:       1257      30    1638

  Fragments arranged by size: 

                1638    1257      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     265     268     857    1205    2925 
   Size:        100     165       3     589     348    1720

  Fragments arranged by size: 

                1720     589     348     165     100       3
 
BseYI C'CCAG_C

Cuts at:      0    2519    2897    2925 
   Size:       2519     378      28

  Fragments arranged by size: 

                2519     378      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    2925 
   Size:        293    2632
 
BsiHKAI G_wGCw'C

Cuts at:      0     335    1391    2246    2925 
   Size:        335    1056     855     679

  Fragments arranged by size: 

                1056     855     679     335
 
BsmI GAATG_Cn'

Cuts at:      0     757    1063    1338    2925 
   Size:        757     306     275    1587

  Fragments arranged by size: 

                1587     757     306     275
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     216    2925 
   Size:        216    2709
 
Bsp1286I G_dGCh'C

Cuts at:      0     281     335    1391    2246    2925 
   Size:        281      54    1056     855     679

  Fragments arranged by size: 

                1056     855     679     281      54
 
BspHI T'CATG_A

Cuts at:      0    2642    2925 
   Size:       2642     283
 
BsrDI GCAATG_nn'

Cuts at:      0     335     603    1471    2925 
   Size:        335     268     868    1454

  Fragments arranged by size: 

                1454     868     335     268
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     238     346    1740    2838    2925 
   Size:        238     108    1394    1098      87

  Fragments arranged by size: 

                1394    1098     238     108      87
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     471    1320    2925 
   Size:        471     849    1605

  Fragments arranged by size: 

                1605     849     471
 
BstYI r'GATC_y

Cuts at:      0       3    2652    2925 
   Size:          3    2649     273

  Fragments arranged by size: 

                2649     273       3
 
BtsI GCAGTG_nn'

Cuts at:      0     106     335     631    2794    2925 
   Size:        106     229     296    2163     131

  Fragments arranged by size: 

                2163     296     229     131     106
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2355    2390    2925 
   Size:       2355      35     535

  Fragments arranged by size: 

                2355     535      35
 
DraI TTT'AAA

Cuts at:      0     198    1422    2210    2925 
   Size:        198    1224     788     715

  Fragments arranged by size: 

                1224     788     715     198
 
EarI CTCTTCn'nnn_

Cuts at:      0    2567    2925 
   Size:       2567     358
 
EcoICRI GAG'CTC

Cuts at:      0    1389    2925 
   Size:       1389    1536
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     240    1151    1247    2791    2925 
   Size:         16     224     911      96    1544     134

  Fragments arranged by size: 

                1544     911     224     134      96      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2660    2925 
   Size:       2660     265
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1309    2775    2925 
   Size:       1309    1466     150

  Fragments arranged by size: 

                1466    1309     150
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1548    2925 
   Size:       1548    1377
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     303     335     935     967    1257    1289    2637    2669 
   Size:        303      32     600      32     290      32    1348      32

Cuts at:   2669    2679    2711    2925 
   Size:         10      32     214

  Fragments arranged by size: 

                1348     600     303     290     214      32      32      32
                  32      32      10
 
HincII GTy'rAC

Cuts at:      0      41     662    1622    1773    2925 
   Size:         41     621     960     151    1152

  Fragments arranged by size: 

                1152     960     621     151      41
 
HpaI GTT'AAC

Cuts at:      0      41     662    1622    2925 
   Size:         41     621     960    1303

  Fragments arranged by size: 

                1303     960     621      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1350    2925 
   Size:       1350    1575
 
MslI CAynn'nnrTG

Cuts at:      0    1318    1729    2925 
   Size:       1318     411    1196

  Fragments arranged by size: 

                1318    1196     411
 
MspA1I CmG'CkG

Cuts at:      0      82    2925 
   Size:         82    2843
 
NdeI CA'TA_TG

Cuts at:      0    1791    2925 
   Size:       1791    1134
 
NsiI A_TGCA'T

Cuts at:      0    1736    2925 
   Size:       1736    1189
 
NspI r_CATG'y

Cuts at:      0    1738    1747    1779    1981    2925 
   Size:       1738       9      32     202     944

  Fragments arranged by size: 

                1738     944     202      32       9
 
PciI A'CATG_T

Cuts at:      0    1977    2925 
   Size:       1977     948
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     159     371    2472    2925 
   Size:        159     212    2101     453

  Fragments arranged by size: 

                2101     453     212     159
 
PfoI T'CCnGG_A

Cuts at:      0     413    2925 
   Size:        413    2512
 
PpuMI rG'GwC_Cy

Cuts at:      0    1309    2775    2925 
   Size:       1309    1466     150

  Fragments arranged by size: 

                1466    1309     150
 
PsiI TTA'TAA

Cuts at:      0     533     998    2611    2672    2925 
   Size:        533     465    1613      61     253

  Fragments arranged by size: 

                1613     533     465     253      61
 
PstI C_TGCA'G

Cuts at:      0    1706    2801    2925 
   Size:       1706    1095     124

  Fragments arranged by size: 

                1706    1095     124
 
PvuII CAG'CTG

Cuts at:      0      82    2925 
   Size:         82    2843
 
SacI G_AGCT'C

Cuts at:      0    1391    2925 
   Size:       1391    1534
 
SfcI C'TryA_G

Cuts at:      0    1702    2222    2354    2797    2902    2925 
   Size:       1702     520     132     443     105      23

  Fragments arranged by size: 

                1702     520     443     132     105      23
 
SmlI C'TyrA_G

Cuts at:      0    1533    2245    2698    2821    2925 
   Size:       1533     712     453     123     104

  Fragments arranged by size: 

                1533     712     453     123     104
 
SpeI A'CTAG_T

Cuts at:      0    2407    2925 
   Size:       2407     518
 
SspI AAT'ATT

Cuts at:      0     141     725    1813    2925 
   Size:        141     584    1088    1112

  Fragments arranged by size: 

                1112    1088     584     141
 
StuI AGG'CCT

Cuts at:      0    2630    2925 
   Size:       2630     295
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1489    2748    2911    2925 
   Size:       1489    1259     163      14

  Fragments arranged by size: 

                1489    1259     163      14
 
TatI w'GTAC_w

Cuts at:      0     186    1278    1954    2925 
   Size:        186    1092     676     971

  Fragments arranged by size: 

                1092     971     676     186
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1821    1853    2925 
   Size:       1821      32    1072

  Fragments arranged by size: 

                1821    1072      32
 
XbaI T'CTAG_A

Cuts at:      0    1284    2925 
   Size:       1284    1641
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     156    2925 
   Size:        156    2769
 
XmnI GAAnn'nnTTC

Cuts at:      0     142     383    2538    2925 
   Size:        142     241    2155     387

  Fragments arranged by size: 

                2155     387     241     142
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI     AloI    AlwNI     ApoI     BaeI
    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI   BstAPI
    BstXI    BstYI     BtsI    CspCI     DraI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I   HincII     HpaI     MmeI     MslI
   MspA1I     NdeI     NsiI     NspI     PciI    PflMI     PfoI    PpuMI
     PsiI     PstI    PvuII     SacI     SfcI     SmlI     SpeI     SspI
     StuI    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI     BbeI     BcgI     BglI    BglII     BlpI Bme1580I
     BmtI     BplI     BsaI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StyI
     SwaI  Tth111I     XhoI     XmaI     ZraI
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