Restriction map for pF1KE1434
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BsaAI       BsaBI       BsaHI       
BsaI        BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       StuI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        941  TCCGGA               T^CCGGA
AclI          930  AACGTT               AA^CGTT
AfaI          860  GTAC                 GT^AC
AocI          140  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2240  AGCGCT               AGC^GCT
AvrII         414  CCTAGG               C^CTAGG
BglII         552  AGATCT               A^GATCT
BlnI          414  CCTAGG               C^CTAGG
BlpI         2305  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2305  GCTCAGC              GC^T[ACGT]AGC
BseAI         941  TCCGGA               T^CCGGA
BsmAI        1219  GAGAC                GTCTC(1/5)
BsmFI        2030  GGGAC                GGGAC(10/14)
BspEI         941  TCCGGA               T^CCGGA
BspMI        1719  GCAGGT               ACCTGC(4/8)
BsrBI        1945  GAGCGG               CCGCTC(-3/-3)
BsrDI          95  GCAATG               GCAATG(2/0)
Bsu36I        140  CCTCAGG              CC^T[ACGT]AGG
CelII        2305  GCTCAGC              GC^T[ACGT]AGC
CfoI         2241  GCGC                 GCG^C
DraII        1817  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI         2124  GAAGAG               CTCTTC(1/4)
Eco47III     2240  AGCGCT               AGC^GCT
Eco81I        140  CCTCAGG              CC^T[ACGT]AGG
EcoNI          48  CCTCTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1817  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1618  GAATTC               G^AATTC
EcoRV        1255  GATATC               GAT^ATC
EcoT22I      1925  ATGCAT               ATGCA^T
HaeII        2240  AGCGCT               [GA]GCGC^[CT]
HhaI         2241  GCGC                 GCG^C
HinP1I       2241  GCGC                 G^CGC
HincII        933  GTTAAC               GT[CT]^[GA]AC
HindII        933  GTTAAC               GT[CT]^[GA]AC
HpaI          933  GTTAAC               GTT^AAC
Ksp632I      2124  GAAGAG               CTCTTC(1/4)
MroI          941  TCCGGA               T^CCGGA
MslI         1705  CATGTGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       2308  CAGCGG               C[AC]G^C[GT]G
NdeI          616  CATATG               CA^TATG
NsiI         1925  ATGCAT               ATGCA^T
NspI         1778  GCATGT               [GA]CATG^[CT]
PpuMI        1817  GGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I      930  AACGTT               AA^CGTT
RsaI          860  GTAC                 GT^AC
SpeI         1503  ACTAGT               A^CTAGT
SspI         1316  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AspHI         984  GAGCAC               G[AT]GC[AT]^C
             1787  GTGCTC              
AvaII        1818  GGTCC                G^G[AT]CC
             1977  GGTCC               
BanII         145  GGGCTC               G[GA]GC[CT]^C
             2212  GAGCCC              
BfaI          415  CTAG                 C^TAG
             1504  CTAG                
BsaWI         941  TCCGGA               [AT]^CCGG[AT]
             1213  ACCGGA              
BsiHKAI       984  GAGCAC               G[AT]GC[AT]^C
             1787  GTGCTC              
BsmI         1524  GAATGC               GAATGC(1/-1)
             1361  GCATTC              
BstXI        1367  CCAAGACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1704  CCATGTGCGTGG        
DraI          126  TTTAAA               TTT^AAA
             1074  TTTAAA              
HapII         942  CCGG                 C^CGG
             1214  CCGG                
HpaII         942  CCGG                 C^CGG
             1214  CCGG                
MaeI          415  CTAG                 C^TAG
             1504  CTAG                
MspI          942  CCGG                 C^CGG
             1214  CCGG                
NcoI         1836  CCATGG               C^CATGG
             2294  CCATGG              
PflMI         234  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1980  CCAGGAGCTGG         
SfcI          214  CTACAG               C^T[GA][CT]AG
              556  CTACAG              
SmlI          526  CTTGAG               C^T[CT][GA]AG
              535  CTTGAG              
Van91I        234  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1980  CCAGGAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        BalI        BbsI        BbvI        BmyI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       
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