Restriction map for pF1KE1434
 (Linear) (Six-base) MAPSORT of: KIEE1434.seq  Check: 3805  from: 1  to: 2352



KIEE1434.orf

 With 182 enzymes: SgfI * 

                        February 19, 2021 20:23  ..

AclI AA'CG_TT

Cuts at:      0     931    2352 
   Size:        931    1421
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533    1594    2352 
   Size:        533    1061     758

  Fragments arranged by size: 

                1061     758     533
 
AfeI AGC'GCT

Cuts at:      0    2242    2352 
   Size:       2242     110
 
AlwNI CAG_nnn'CTG

Cuts at:      0     157     240    1986    2352 
   Size:        157      83    1746     366

  Fragments arranged by size: 

                1746     366     157      83
 
ApoI r'AATT_y

Cuts at:      0     741    1107    1134    1379    1422    1618    2352 
   Size:        741     366      27     245      43     196     734

  Fragments arranged by size: 

                 741     734     366     245     196      43      27
 
AvrII C'CTAG_G

Cuts at:      0     414    2352 
   Size:        414    1938
 
BanII G_rGCy'C

Cuts at:      0     149    2216    2352 
   Size:        149    2067     136

  Fragments arranged by size: 

                2067     149     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     341    1582    2011    2352 
   Size:        341    1241     429     341

  Fragments arranged by size: 

                1241     429     341     341
 
BfrBI ATG'CAT

Cuts at:      0    1927    2352 
   Size:       1927     425
 
BglII A'GATC_T

Cuts at:      0     552    2352 
   Size:        552    1800
 
BlpI GC'TnA_GC

Cuts at:      0    2306    2352 
   Size:       2306      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     403    1968    2352 
   Size:        403    1565     384

  Fragments arranged by size: 

                1565     403     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     661    1858    2008    2230    2305    2352 
   Size:        217     444    1197     150     222      75      47

  Fragments arranged by size: 

                1197     444     222     217     150      75      47
 
Bpu10I CC'TnA_GC

Cuts at:      0     117    2352 
   Size:        117    2235
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     556    2352 
   Size:        547       9    1796

  Fragments arranged by size: 

                1796     547       9
 
BsaWI w'CCGG_w

Cuts at:      0     941    1213    2352 
   Size:        941     272    1139

  Fragments arranged by size: 

                1139     941     272
 
BseYI C'CCAG_C

Cuts at:      0    1903    2237    2352 
   Size:       1903     334     115

  Fragments arranged by size: 

                1903     334     115
 
BsiHKAI G_wGCw'C

Cuts at:      0     988    1791    2352 
   Size:        988     803     561

  Fragments arranged by size: 

                 988     803     561
 
BsmI GAATG_Cn'

Cuts at:      0    1361    1530    2352 
   Size:       1361     169     822

  Fragments arranged by size: 

                1361     822     169
 
Bsp1286I G_dGCh'C

Cuts at:      0     149     988    1791    2216    2352 
   Size:        149     839     803     425     136

  Fragments arranged by size: 

                 839     803     425     149     136
 
BspEI T'CCGG_A

Cuts at:      0     941    2352 
   Size:        941    1411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1710    2352 
   Size:       1710     642
 
BsrBI CCG'CTC

Cuts at:      0    1947    2352 
   Size:       1947     405
 
BsrDI GCAATG_nn'

Cuts at:      0     102    2352 
   Size:        102    2250
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1374    1711    2352 
   Size:       1374     337     641

  Fragments arranged by size: 

                1374     641     337
 
BstYI r'GATC_y

Cuts at:      0     121     552     912    1095    2352 
   Size:        121     431     360     183    1257

  Fragments arranged by size: 

                1257     431     360     183     121
 
Bsu36I CC'TnA_GG

Cuts at:      0     141    2352 
   Size:        141    2211
 
BtgI C'CryG_G

Cuts at:      0     668    1836    2294    2352 
   Size:        668    1168     458      58

  Fragments arranged by size: 

                1168     668     458      58
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      28     650     685    2352 
   Size:         28     622      35    1667

  Fragments arranged by size: 

                1667     622      35      28
 
DraI TTT'AAA

Cuts at:      0     128    1076    2352 
   Size:        128     948    1276

  Fragments arranged by size: 

                1276     948     128
 
EaeI y'GGCC_r

Cuts at:      0    1300    1652    1672    1713    2352 
   Size:       1300     352      20      41     639

  Fragments arranged by size: 

                1300     639     352      41      20
 
EarI CTCTTCn'nnn_

Cuts at:      0    2119    2352 
   Size:       2119     233
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1723    2352 
   Size:       1723     629
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     533     661    1594    1858    2008    2230    2305 
   Size:        217     316     128     933     264     150     222      75

Cuts at:   2305    2352 
   Size:         47

  Fragments arranged by size: 

                 933     316     264     222     217     150     128      75
                  47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      52    2352 
   Size:         52    2300
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1818    2352 
   Size:       1818     534
 
EcoRI G'AATT_C

Cuts at:      0    1618    2352 
   Size:       1618     734
 
EcoRV GAT'ATC

Cuts at:      0    1257    2352 
   Size:       1257    1095
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2049    2352 
   Size:       2049     303
 
HaeII r_GCGC'y

Cuts at:      0    2244    2352 
   Size:       2244     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1684    1716    2352 
   Size:       1684      32     636

  Fragments arranged by size: 

                1684     636      32
 
HincII GTy'rAC

Cuts at:      0     935    2352 
   Size:        935    1417
 
HpaI GTT'AAC

Cuts at:      0     935    2352 
   Size:        935    1417
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    1727    2352 
   Size:        203    1524     625

  Fragments arranged by size: 

                1524     625     203
 
MscI TGG'CCA

Cuts at:      0    1302    1654    1674    1715    2352 
   Size:       1302     352      20      41     637

  Fragments arranged by size: 

                1302     637     352      41      20
 
MslI CAynn'nnrTG

Cuts at:      0    1709    2352 
   Size:       1709     643
 
MspA1I CmG'CkG

Cuts at:      0    2310    2352 
   Size:       2310      42
 
NcoI C'CATG_G

Cuts at:      0    1836    2294    2352 
   Size:       1836     458      58

  Fragments arranged by size: 

                1836     458      58
 
NdeI CA'TA_TG

Cuts at:      0     617    2352 
   Size:        617    1735
 
NsiI A_TGCA'T

Cuts at:      0    1929    2352 
   Size:       1929     423
 
NspI r_CATG'y

Cuts at:      0    1782    2352 
   Size:       1782     570
 
PasI CC'CwG_GG

Cuts at:      0     294    2352 
   Size:        294    2058
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240    1986    2352 
   Size:        240    1746     366

  Fragments arranged by size: 

                1746     366     240
 
PfoI T'CCnGG_A

Cuts at:      0    1979    2352 
   Size:       1979     373
 
PpuMI rG'GwC_Cy

Cuts at:      0    1818    2352 
   Size:       1818     534
 
SfcI C'TryA_G

Cuts at:      0     214     556    2352 
   Size:        214     342    1796

  Fragments arranged by size: 

                1796     342     214
 
SmlI C'TyrA_G

Cuts at:      0     526     535    2352 
   Size:        526       9    1817

  Fragments arranged by size: 

                1817     526       9
 
SpeI A'CTAG_T

Cuts at:      0    1503    2352 
   Size:       1503     849
 
SspI AAT'ATT

Cuts at:      0    1318    2352 
   Size:       1318    1034
 
StyI C'CwwG_G

Cuts at:      0      53     414     958    1836    2294    2352 
   Size:         53     361     544     878     458      58

  Fragments arranged by size: 

                 878     544     458     361      58      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1950    2352 
   Size:       1950     402
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      11    1834    2280    2352 
   Size:         11    1823     446      72

  Fragments arranged by size: 

                1823     446      72      11
 

 Enzymes that do cut:

     AclI     AcuI     AfeI    AlwNI     ApoI    AvrII    BanII     BbsI
    BfrBI    BglII     BlpI     BmrI     BpmI   Bpu10I    BpuEI    BsaWI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BstXI    BstYI   Bsu36I     BtgI    CspCI     DraI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I   HincII     HpaI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI     PasI    PflMI     PfoI    PpuMI     SfcI
     SmlI     SpeI     SspI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspHI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     BtsI     ClaI   DraIII     DrdI     EagI  EcoICRI
     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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