Restriction map for pF1KB3356
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AlwNI       AocI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaII       AviII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HapII       HgaI        Hin1I       
HpaI        HpaII       KasI        KpnI        MamI        
MluI        MluNI       MroI        MscI        MspI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          533  GTATAC               GT^[AC][GT]AC
AccII          32  CGCG                 CG^CG
AflIII        772  ACATGT               A^C[GA][CT]GT
Alw44I       2183  GTGCAC               G^TGCAC
Aor51HI      2480  AGCGCT               AGC^GCT
ApaLI        2183  GTGCAC               G^TGCAC
AvaI         2099  CCCGAG               C^[CT]CG[GA]G
AvrII         741  CCTAGG               C^CTAGG
BamHI         295  GGATCC               G^GATCC
BglI         1316  GCCCAAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         593  AGATCT               A^GATCT
BlnI          741  CCTAGG               C^CTAGG
BsaI         1860  GGTCTC               GGTCTC(1/5)
BsmI         1077  GAATGC               GAATGC(1/-1)
BsoBI        2099  CCCGAG               C^[CT]CG[GA]G
BspLU11I      772  ACATGT               A^CATGT
Bst1107I      533  GTATAC               GTA^TAC
BstUI          32  CGCG                 CG^CG
BstZ17I       533  GTATAC               GTA^TAC
CfoI         2481  GCGC                 GCG^C
Cfr13I       2470  GGCCC                G^G[ACGT]CC
DraIII        167  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2480  AGCGCT               AGC^GCT
EcoNI        1780  CCTATGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII        2480  AGCGCT               [GA]GCGC^[CT]
HhaI         2481  GCGC                 GCG^C
HinP1I       2481  GCGC                 G^CGC
KspI           31  CCGCGG               CCGC^GG
MspA1I         31  CCGCGG               C[AC]G^C[GT]G
MvnI           32  CGCG                 CG^CG
NcoI         1129  CCATGG               C^CATGG
NheI         1737  GCTAGC               G^CTAGC
PflMI         769  CCAACATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII          31  CCGCGG               CCGC^GG
Sau96I       2470  GGCCC                G^G[ACGT]CC
SexAI         856  ACCTGGT              A^CC[AT]GGT
SfaNI        1999  GCATC                GCATC(5/9)
SfcI          225  CTGTAG               C^T[GA][CT]AG
SmlI         1415  CTCAAG               C^T[CT][GA]AG
TaqI         2463  TCGA                 T^CGA
TthHB8I      2463  TCGA                 T^CGA
Van91I        769  CCAACATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          442  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI         1249  GAATTT               [GA]^AATT[CT]
             1362  AAATTT              
ApoI         1249  GAATTT               [GA]^AATT[CT]
             1362  AAATTT              
Asp700I      1246  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1684  GAAGATTTTC          
BclI          504  TGATCA               T^GATCA
              876  TGATCA              
BseRI        1634  CTCCTC               GAGGAG(10/8)
              964  GAGGAG              
BsmFI        1885  GTCCC                GGGAC(10/14)
             2035  GTCCC               
BstXI         538  CCAGAAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1107  CCAAGTAGATGG        
DraI          708  TTTAAA               TTT^AAA
              910  TTTAAA              
EcoT14I       741  CCTAGG               C^C[AT][AT]GG
             1129  CCATGG              
FbaI          504  TGATCA               T^GATCA
              876  TGATCA              
HindIII       979  AAGCTT               A^AGCTT
             1549  AAGCTT              
NspI          772  ACATGT               [GA]CATG^[CT]
             2071  GCATGT              
SspI          343  AATATT               AAT^ATT
              791  AATATT              
StyI          741  CCTAGG               C^C[AT][AT]GG
             1129  CCATGG              
Tsp45I       2131  GTGAC                ^GT[GC]AC
             2168  GTCAC               
XmnI         1246  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1684  GAAGATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       DdeI        DpnII       DsaI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MunI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
ScrFI       TfiI        Tru9I       TseI        Tsp509I     
TspRI       XhoII       
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