Restriction map for pF1KB4538
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BbvI        BcgI        BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        ClaI        CpoI        DraII       DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TseI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII          11  GACGTC               GACGT^C
AcsI          595  AAATTT               [GA]^AATT[CT]
AcyI           11  GACGTC               G[GA]^CG[CT]C
AlwI          825  GATCC                GGATC(4/5)
AlwNI         707  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          595  AAATTT               [GA]^AATT[CT]
Asp700I       420  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1175  GTGCTC               G[AT]GC[AT]^C
AspI          970  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          163  CCCGAG               C^[CT]CG[GA]G
BciVI          35  GTATCC               GTATCC(6/5)
BcnI          483  CCGGG                CC^[GC]GG
BmyI         1175  GTGCTC               G[AGT]GC[ACT]^C
BsaHI          11  GACGTC               G[GA]^CG[CT]C
BsaI         1160  GGTCTC               GGTCTC(1/5)
BseRI        1075  GAGGAG               GAGGAG(10/8)
BsiHKAI      1175  GTGCTC               G[AT]GC[AT]^C
BsmFI         447  GTCCC                GGGAC(10/14)
BsoBI         163  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     1175  GTGCTC               G[AGT]GC[ACT]^C
BsrFI         200  ACCGGC               [GA]^CCGG[CT]
BstXI         828  CCACAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        200  ACCGGC               [GA]^CCGG[CT]
DrdI          295  GACCTGAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          936  CGGCCA               [CT]^GGCC[GA]
EarI          808  CTCTTC               CTCTTC(1/4)
FauI          250  GCGGG                CCCGC(4/6)
Fnu4HI        374  GCCGC                GC^[ACGT]GC
Hin1I          11  GACGTC               G[GA]^CG[CT]C
HphI          817  GGTGA                GGTGA(8/7)
ItaI          374  GCCGC                GC^[ACGT]GC
Ksp632I       808  CTCTTC               CTCTTC(1/4)
NciI          483  CCGGG                CC^[GC]GG
NlaIII        225  CATG                 CATG^
NspI          224  GCATGC               [GA]CATG^[CT]
PflFI         970  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         519  CCAAACGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          533  GAGTC                GAGTC(4/5)
SexAI         971  ACCAGGT              A^CC[AT]GGT
SfaNI         371  GATGC                GCATC(5/9)
SfcI          636  CTACAG               C^T[GA][CT]AG
SmlI         1139  CTCAAG               C^T[CT][GA]AG
SphI          224  GCATGC               GCATG^C
TfiI          487  GAATC                G^A[AT]TC
Tsp45I        304  GTCAC                ^GT[GC]AC
Tth111I       970  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        519  CCAAACGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           96  CCAAGAAAAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          420  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          375  CCGC                 CCGC(-3/-1)
              250  GCGG                
AluI          194  AGCT                 AG^CT
              857  AGCT                
AocI          297  CCTGAGG              CC^T[ACGT]AGG
              948  CCTGAGG             
AvaII         838  GGACC                G^G[AT]CC
              975  GGTCC               
BbsI           55  GTCTTC               GAAGAC(2/6)
              535  GTCTTC              
BglII         804  AGATCT               A^GATCT
             1110  AGATCT              
BpuAI          55  GTCTTC               GAAGAC(2/6)
              535  GTCTTC              
BsaJI         164  CCGAGG               C^C[ACGT][ACGT]GG
              491  CCTGGG              
BsrI          173  ACTGG                ACTGG(1/-1)
             1006  ACTGG               
BstYI         804  AGATCT               [GA]^GATC[CT]
             1110  AGATCT              
Bsu36I        297  CCTGAGG              CC^T[ACGT]AGG
              948  CCTGAGG             
DraI          346  TTTAAA               TTT^AAA
             1182  TTTAAA              
Eco81I        297  CCTGAGG              CC^T[ACGT]AGG
              948  CCTGAGG             
EcoRV         150  GATATC               GAT^ATC
             1069  GATATC              
HinfI         487  GAATC                G^A[ACGT]TC
              533  GAGTC               
MaeIII        304  GTCAC                ^GT[ACGT]AC
              316  GTTAC               
MflI          804  AGATCT               [GA]^GATC[CT]
             1110  AGATCT              
MslI          306  CACGTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              621  CATTCTTATG          
ScaI          694  AGTACT               AGT^ACT
              863  AGTACT              
TaqI           15  TCGA                 T^CGA
             1073  TCGA                
TthHB8I        15  TCGA                 T^CGA
             1073  TCGA                
XhoII         804  AGATCT               [GA]^GATC[CT]
             1110  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AfaI        BsiYI       BslI        BsmAI       BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      FokI        HaeIII      HapII       
HpaII       MaeII       MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
Tru9I       Tsp509I     TspRI       
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