Restriction map for pF1KE1355
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       EaeI        EagI        EclXI       Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspI        StyI        
SwaI        TfiI        Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          514  GTCTAC               GT^[AC][GT]AC
AfaI          315  GTAC                 GT^AC
AhdI          325  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         325  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          474  CCCGGG               C^[CT]CG[GA]G
AvaII         174  GGTCC                G^G[AT]CC
BanI          733  GGCGCC               G^G[CT][GA]CC
BanII         370  GAGCCC               G[GA]GC[CT]^C
BbeI          733  GGCGCC               GGCGC^C
BcgI          567  CGACTTCAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          370  GAGCCC               G[AGT]GC[ACT]^C
BsaAI         259  TACGTG               [CT]AC^GT[GA]
BsgI          142  GTGCAG               GTGCAG(16/14)
BsoBI         474  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      370  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      314  TGTACA               T^GTACA
BsrGI         314  TGTACA               T^GTACA
BstXI          90  CCAGACATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         146  AGATCC               [GA]^GATC[CT]
DraIII        256  CACTACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          105  GACCAAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          206  CCGTGG               C^C[GA][CT]GG
Eam1105I      325  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          755  CTCTTC               CTCTTC(1/4)
HinfI         748  GACTC                G^A[ACGT]TC
HphI          834  GGTGA                GGTGA(8/7)
KasI          733  GGCGCC               G^GCGCC
Ksp632I       755  CTCTTC               CTCTTC(1/4)
MflI          146  AGATCC               [GA]^GATC[CT]
MseI          764  TTAA                 T^TAA
MslI          555  CATGTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          733  GGCGCC               GG^CGCC
NspI          465  GCATGC               [GA]CATG^[CT]
PleI          748  GACTC                GAGTC(4/5)
RsaI          315  GTAC                 GT^AC
SapI          754  GCTCTTC              GCTCTTC(1/4)
SexAI         326  ACCAGGT              A^CC[AT]GGT
SfoI          733  GGCGCC               GGC^GCC
SmaI          474  CCCGGG               CCC^GGG
SphI          465  GCATGC               GCATG^C
SspBI         314  TGTACA               T^GTACA
StuI          319  AGGCCT               AGG^CCT
TaqI          497  TCGA                 T^CGA
Tru9I         764  TTAA                 T^TAA
TthHB8I       497  TCGA                 T^CGA
XcmI          771  CCAGGCTGCCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         146  AGATCC               [GA]^GATC[CT]
XmaCI         474  CCCGGG               C^CCGGG
XmaI          474  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AatII         511  GACGTC               GACGT^C
              550  GACGTC              
AlwNI         145  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              643  CAGTGGCTG           
Aor51HI       299  AGCGCT               AGC^GCT
              661  AGCGCT              
BsaI          103  GAGACC               GGTCTC(1/5)
              594  GAGACC              
DdeI          151  CTGAG                C^T[ACGT]AG
              787  CTGAG               
Eco47III      299  AGCGCT               AGC^GCT
              661  AGCGCT              
FauI          377  GCGGG                CCCGC(4/6)
              577  GCGGG               
HapII         426  CCGG                 C^CGG
              475  CCGG                
HgaI          481  GACGC                GACGC(5/10)
              499  GACGC               
HpaII         426  CCGG                 C^CGG
              475  CCGG                
MaeIII        791  GTGAC                ^GT[ACGT]AC
              815  GTGAC               
MspI          426  CCGG                 C^CGG
              475  CCGG                
PstI           49  CTGCAG               CTGCA^G
              582  CTGCAG              
SfcI           49  CTGCAG               C^T[GA][CT]AG
              582  CTGCAG              
Tsp45I        791  GTGAC                ^GT[GC]AC
              815  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        BbvI        
BcnI        BsaHI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DpnII       
EcoNI       EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HhaI        Hin1I       HinP1I      ItaI        
MaeII       MboI        MboII       MnlI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
TspRI       
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