Restriction map for pF1KE1355
 (Linear) (Six-base) MAPSORT of: KIEE1355.seq  Check: 1259  from: 1  to: 846



KIEE1355.orf

 With 182 enzymes: SgfI * 

                        November 21, 2014 11:13  ..

AatII G_ACGT'C

Cuts at:      0     515     554     846 
   Size:        515      39     292

  Fragments arranged by size: 

                 515     292      39
 
AccI GT'mk_AC

Cuts at:      0     515     846 
   Size:        515     331
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     846 
   Size:         88     758
 
AfeI AGC'GCT

Cuts at:      0     301     663     846 
   Size:        301     362     183

  Fragments arranged by size: 

                 362     301     183
 
AhdI GACnn_n'nnGTC

Cuts at:      0     330     846 
   Size:        330     516
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      31     846 
   Size:         31     815
 
AlwNI CAG_nnn'CTG

Cuts at:      0     150     648     846 
   Size:        150     498     198

  Fragments arranged by size: 

                 498     198     150
 
AvaI C'yCGr_G

Cuts at:      0     474     846 
   Size:        474     372
 
BanI G'GyrC_C

Cuts at:      0     733     846 
   Size:        733     113
 
BanII G_rGCy'C

Cuts at:      0     374     846 
   Size:        374     472
 
BbeI G_GCGC'C

Cuts at:      0     737     846 
   Size:        737     109
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     556     590     846 
   Size:        556      34     256

  Fragments arranged by size: 

                 556     256      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     286     597     846 
   Size:        286     311     249

  Fragments arranged by size: 

                 311     286     249
 
Bpu10I CC'TnA_GC

Cuts at:      0     151     846 
   Size:        151     695
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      97     588     846 
   Size:         97     491     258

  Fragments arranged by size: 

                 491     258      97
 
BsaAI yAC'GTr

Cuts at:      0     261     846 
   Size:        261     585
 
BsaHI Gr'CG_yC

Cuts at:      0     512     551     734     846 
   Size:        512      39     183     112

  Fragments arranged by size: 

                 512     183     112      39
 
BseYI C'CCAG_C

Cuts at:      0      18     846 
   Size:         18     828
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     846 
   Size:        163     683
 
Bsp1286I G_dGCh'C

Cuts at:      0     374     846 
   Size:        374     472
 
BsrGI T'GTAC_A

Cuts at:      0     314     846 
   Size:        314     532
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      97     846 
   Size:         97     749
 
BstYI r'GATC_y

Cuts at:      0     146     846 
   Size:        146     700
 
BtgI C'CryG_G

Cuts at:      0     206     846 
   Size:        206     640
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     150     846 
   Size:        150     696
 
DraIII CAC_nnn'GTG

Cuts at:      0     261     846 
   Size:        261     585
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     111     846 
   Size:        111     735
 
EarI CTCTTCn'nnn_

Cuts at:      0     761     846 
   Size:        761      85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     607     846 
   Size:        607     239
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     846 
   Size:         88     758
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      49     175     232     699     846 
   Size:         49     126      57     467     147

  Fragments arranged by size: 

                 467     147     126      57      49
 
HaeII r_GCGC'y

Cuts at:      0      28     303     665     737     846 
   Size:         28     275     362      72     109

  Fragments arranged by size: 

                 362     275     109      72      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     486     518     846 
   Size:        316      32     138      32     328

  Fragments arranged by size: 

                 328     316     138      32      32
 
KasI G'GCGC_C

Cuts at:      0     733     846 
   Size:        733     113
 
MslI CAynn'nnrTG

Cuts at:      0     559     846 
   Size:        559     287
 
NarI GG'CG_CC

Cuts at:      0     734     846 
   Size:        734     112
 
NspI r_CATG'y

Cuts at:      0     469     846 
   Size:        469     377
 
PfoI T'CCnGG_A

Cuts at:      0     176     846 
   Size:        176     670
 
PstI C_TGCA'G

Cuts at:      0      53     586     846 
   Size:         53     533     260

  Fragments arranged by size: 

                 533     260      53
 
SapI GCTCTTCn'nnn_

Cuts at:      0     761     846 
   Size:        761      85
 
SexAI A'CCwGG_T

Cuts at:      0     326     846 
   Size:        326     520
 
SfcI C'TryA_G

Cuts at:      0      49     582     846 
   Size:         49     533     264

  Fragments arranged by size: 

                 533     264      49
 
SfoI GGC'GCC

Cuts at:      0     735     846 
   Size:        735     111
 
SmaI CCC'GGG

Cuts at:      0     476     846 
   Size:        476     370
 
SphI G_CATG'C

Cuts at:      0     469     846 
   Size:        469     377
 
StuI AGG'CCT

Cuts at:      0     321     846 
   Size:        321     525
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     696     846 
   Size:        696     150
 
TatI w'GTAC_w

Cuts at:      0     314     846 
   Size:        314     532
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     778     846 
   Size:        778      68
 
XmaI C'CCGG_G

Cuts at:      0     474     846 
   Size:        474     372
 
ZraI GAC'GTC

Cuts at:      0     513     552     846 
   Size:        513      39     294

  Fragments arranged by size: 

                 513     294      39
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AhdI     AlfI    AlwNI     AvaI
     BanI    BanII     BbeI     BcgI     BmrI   Bpu10I     BsaI    BsaAI
    BsaHI    BseYI     BsgI Bsp1286I    BsrGI    BstXI    BstYI     BtgI
    BtgZI   DraIII     DrdI     EarI     EciI  Eco57MI    EcoNI    HaeII
    Hin4I     KasI     MslI     NarI     NspI     PfoI     PstI     SapI
    SexAI     SfcI     SfoI     SmaI     SphI     StuI    TaqII     TatI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AleI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaBI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI     EaeI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmnI
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