Restriction map for
pF1KE0705
(Circular) (Six-base) MAPSORT of: pF1KE0705.seq Check: 9328 from: 1 to: 3998
pF1KE0705 3998 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 112 1036 112
Size: 924 3074
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 797 1823 2255 3002 3270 451
Size: 346 1026 432 747 268 1179
Fragments arranged by size:
1179 1026 747 432 346 268
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AleI CACnn'nnGTG
Cuts at: 687 687
Size: 3998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 775 1303 2870 775
Size: 528 1567 1903
Fragments arranged by size:
1903 1567 528
ApaI G_GGCC'C
Cuts at: 442 442
Size: 3998
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 411 459 624 1002 3351 3607 411
Size: 48 165 378 2349 256 802
Fragments arranged by size:
2349 802 378 256 165 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 1018 1018
Size: 3998
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 857 890 857
Size: 33 3965
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 442 1012 2042 442
Size: 570 1030 2398
Fragments arranged by size:
2398 1030 570
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 442 1623 1716 2772 442
Size: 1181 93 1056 1668
Fragments arranged by size:
1668 1181 1056 93
BmgBI CAC'GTC
Cuts at: 378 487 378
Size: 109 3889
BmrI ACTGGGnnnn_n'
Cuts at: 159 1210 1291 1626 3770 159
Size: 1051 81 335 2144 387
Fragments arranged by size:
2144 1051 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 132
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 1174 2053 2545 2843 908
Size: 266 879 492 298 2063
Fragments arranged by size:
2063 879 492 298 266
BsaI GGTCTCn'nnnn_
Cuts at: 3702 3702
Size: 3998
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 764 794 764
Size: 30 3968
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 500 500
Size: 3998
BseYI C'CCAG_C
Cuts at: 435 1423 2181 2758 3254 435
Size: 988 758 577 496 1179
Fragments arranged by size:
1179 988 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 499 406
Size: 93 3905
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1012 1790 1980 2772 1012
Size: 778 190 792 2238
Fragments arranged by size:
2238 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 442 1012 1623 1716 1790 1980 2042 2772 442
Size: 570 611 93 74 190 62 730 1668
Fragments arranged by size:
1668 730 611 570 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 804 3174 804
Size: 2370 1628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 983 1910 983
Size: 927 3071
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 689 689
Size: 3998
BstYI r'GATC_y
Cuts at: 1 1023 1490 1513 1848 2094 3106 3192 3204
Size: 1022 467 23 335 246 1012 86 12
Cuts at: 3204 3550 1
Size: 346 449
Fragments arranged by size:
1022 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 417 417
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 1241 1241
Size: 3998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 473 508 473
Size: 35 3963
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 1583 1757 2148 2175 3520 1583
Size: 174 391 27 1345 2061
Fragments arranged by size:
2061 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 451 797 1823 2255 3002 3270 132
Size: 319 346 1026 432 747 268 860
Fragments arranged by size:
1026 860 747 432 346 319 268
EcoO109I rG'GnC_Cy
Cuts at: 439 1137 439
Size: 698 3300
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
EcoRV GAT'ATC
Cuts at: 652 652
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 1432 1680 2702 3681 1432
Size: 248 1022 979 1749
Fragments arranged by size:
1749 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1840 1870 1872 1902 1840
Size: 30 2 30 3936
Fragments arranged by size:
3936 30 30 2
HincII GTy'rAC
Cuts at: 744 1037 744
Size: 293 3705
HindIII A'AGCT_T
Cuts at: 279 279
Size: 3998
HpaI GTT'AAC
Cuts at: 744 744
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 219 2669 2853 219
Size: 2450 184 1364
Fragments arranged by size:
2450 1364 184
MscI TGG'CCA
Cuts at: 1759 1759
Size: 3998
MslI CAynn'nnrTG
Cuts at: 687 755 1987 2114 687
Size: 68 1232 127 2571
Fragments arranged by size:
2571 1232 127 68
MspA1I CmG'CkG
Cuts at: 178 739 1109 1423 1783 2796 3041 178
Size: 561 370 314 360 1013 245 1135
Fragments arranged by size:
1135 1013 561 370 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 417 417
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 1051 1551 2458 1051
Size: 500 907 2591
Fragments arranged by size:
2591 907 500
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PflMI CCAn_nnn'nTGG
Cuts at: 823 823
Size: 3998
PfoI T'CCnGG_A
Cuts at: 768 768
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PspOMI G'GGCC_C
Cuts at: 438 438
Size: 3998
PstI C_TGCA'G
Cuts at: 1045 1730 1045
Size: 685 3313
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 178 739 1423 1783 178
Size: 561 684 360 2393
Fragments arranged by size:
2393 684 561 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 1041 1726 2719 2910 33
Size: 1008 685 993 191 1121
Fragments arranged by size:
1121 1008 993 685 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 923 1153 2068 2560 2822 923
Size: 230 915 492 262 2099
Fragments arranged by size:
2099 915 492 262 230
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StyI C'CwwG_G
Cuts at: 189 417 1132 1486 189
Size: 228 715 354 2701
Fragments arranged by size:
2701 715 354 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 178 869 1581 1714 2173 2382 178
Size: 691 712 133 459 209 1794
Fragments arranged by size:
1794 712 691 459 209 133
TatI w'GTAC_w
Cuts at: 3513 3513
Size: 3998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 567 599 3515 3547 567
Size: 32 2916 32 1018
Fragments arranged by size:
2916 1018 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoNI FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI