Restriction map for pF1KB6668
 (Linear) (Six-base) MAPSORT of: KIBB6668.seq  Check: 9427  from: 1  to: 1359



KIBB6668.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:      0     314    1333    1359 
   Size:        314    1019      26

  Fragments arranged by size: 

                1019     314      26
 
AclI AA'CG_TT

Cuts at:      0     177    1359 
   Size:        177    1182
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     991    1091    1359 
   Size:        202     789     100     268

  Fragments arranged by size: 

                 789     268     202     100
 
AflIII A'CryG_T

Cuts at:      0      54    1359 
   Size:         54    1305
 
AhdI GACnn_n'nnGTC

Cuts at:      0     733    1359 
   Size:        733     626
 
AlwNI CAG_nnn'CTG

Cuts at:      0     100     699    1272    1359 
   Size:        100     599     573      87

  Fragments arranged by size: 

                 599     573     100      87
 
BanI G'GyrC_C

Cuts at:      0     477    1359 
   Size:        477     882
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     201     721    1290    1359 
   Size:        201     520     569      69

  Fragments arranged by size: 

                 569     520     201      69
 
BbvCI CC'TCA_GC

Cuts at:      0     143    1289    1359 
   Size:        143    1146      70

  Fragments arranged by size: 

                1146     143      70
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38    1359 
   Size:          4      34    1321

  Fragments arranged by size: 

                1321      34       4
 
BfrBI ATG'CAT

Cuts at:      0     277    1359 
   Size:        277    1082
 
BglI GCCn_nnn'nGGC

Cuts at:      0     888    1359 
   Size:        888     471
 
BglII A'GATC_T

Cuts at:      0     485     804    1316    1359 
   Size:        485     319     512      43

  Fragments arranged by size: 

                 512     485     319      43
 
Bme1580I G_kGCm'C

Cuts at:      0     480    1258    1359 
   Size:        480     778     101

  Fragments arranged by size: 

                 778     480     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1048    1359 
   Size:       1048     311
 
Bpu10I CC'TnA_GC

Cuts at:      0     143    1289    1359 
   Size:        143    1146      70

  Fragments arranged by size: 

                1146     143      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     972    1359 
   Size:        972     387
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1041    1359 
   Size:       1041     318
 
BsaHI Gr'CG_yC

Cuts at:      0     734    1228    1359 
   Size:        734     494     131

  Fragments arranged by size: 

                 734     494     131
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     367    1219    1359 
   Size:        367     852     140

  Fragments arranged by size: 

                 852     367     140
 
BseYI C'CCAG_C

Cuts at:      0      63    1359 
   Size:         63    1296
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1329    1359 
   Size:       1329      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     480    1258    1359 
   Size:        480     778     101

  Fragments arranged by size: 

                 778     480     101
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      20    1359 
   Size:         20    1339
 
BstEII G'GTnAC_C

Cuts at:      0     453    1359 
   Size:        453     906
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     531    1359 
   Size:        531     828
 
BstYI r'GATC_y

Cuts at:      0     485     804    1100    1316    1359 
   Size:        485     319     296     216      43

  Fragments arranged by size: 

                 485     319     296     216      43
 
BtsI GCAGTG_nn'

Cuts at:      0     399    1359 
   Size:        399     960
 
DraI TTT'AAA

Cuts at:      0     167    1359 
   Size:        167    1192
 
EaeI y'GGCC_r

Cuts at:      0     291     743    1359 
   Size:        291     452     616

  Fragments arranged by size: 

                 616     452     291
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     991    1048    1091    1359 
   Size:        202     789      57      43     268

  Fragments arranged by size: 

                 789     268     202      57      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     194     352     655    1063    1359 
   Size:        194     158     303     408     296

  Fragments arranged by size: 

                 408     303     296     194     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0     437     606    1209    1359 
   Size:        437     169     603     150

  Fragments arranged by size: 

                 603     437     169     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      73     105     719     751    1359 
   Size:         73      32     614      32     608

  Fragments arranged by size: 

                 614     608      73      32      32
 
HincII GTy'rAC

Cuts at:      0     315     543    1359 
   Size:        315     228     816

  Fragments arranged by size: 

                 816     315     228
 
MscI TGG'CCA

Cuts at:      0     293     745    1359 
   Size:        293     452     614

  Fragments arranged by size: 

                 614     452     293
 
MslI CAynn'nnrTG

Cuts at:      0     833    1359 
   Size:        833     526
 
MspA1I CmG'CkG

Cuts at:      0    1218    1359 
   Size:       1218     141
 
NsiI A_TGCA'T

Cuts at:      0     279    1359 
   Size:        279    1080
 
NspI r_CATG'y

Cuts at:      0      58    1359 
   Size:         58    1301
 
PciI A'CATG_T

Cuts at:      0      54    1359 
   Size:         54    1305
 
PfoI T'CCnGG_A

Cuts at:      0     530     944    1064    1359 
   Size:        530     414     120     295

  Fragments arranged by size: 

                 530     414     295     120
 
PpuMI rG'GwC_Cy

Cuts at:      0     437    1209    1359 
   Size:        437     772     150

  Fragments arranged by size: 

                 772     437     150
 
PstI C_TGCA'G

Cuts at:      0      17      38     473     507     785    1359 
   Size:         17      21     435      34     278     574

  Fragments arranged by size: 

                 574     435     278      34      21      17
 
SalI G'TCGA_C

Cuts at:      0     313    1359 
   Size:        313    1046
 
SbfI CC_TGCA'GG

Cuts at:      0      17     473    1359 
   Size:         17     456     886

  Fragments arranged by size: 

                 886     456      17
 
SfcI C'TryA_G

Cuts at:      0      13      34     469     503     723     781    1359 
   Size:         13      21     435      34     220      58     578

  Fragments arranged by size: 

                 578     435     220      58      34      21      13
 
SmlI C'TyrA_G

Cuts at:      0     987    1359 
   Size:        987     372
 
StuI AGG'CCT

Cuts at:      0     775    1359 
   Size:        775     584
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     472    1045    1359 
   Size:        472     573     314

  Fragments arranged by size: 

                 573     472     314
 
Tth111I GACn'n_nGTC

Cuts at:      0     205     538    1193    1359 
   Size:        205     333     655     166

  Fragments arranged by size: 

                 655     333     205     166
 
XbaI T'CTAG_A

Cuts at:      0    1260    1359 
   Size:       1260      99
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1143    1239    1359 
   Size:       1143      96     120

  Fragments arranged by size: 

                1143     120      96
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AhdI    AlwNI     BanI     BbsI
    BbvCI     BcgI    BfrBI     BglI    BglII Bme1580I     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI Bsp1286I    BspMI
   BstEII    BstXI    BstYI     BtsI     DraI     EaeI  Eco57MI    EcoNI
 EcoO109I    Hin4I   HincII     MscI     MslI   MspA1I     NsiI     NspI
     PciI     PfoI    PpuMI     PstI     SalI     SbfI     SfcI     SmlI
     StuI    TaqII  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BciVI     BclI     BlpI
    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TatI     TstI     XhoI     XmaI     XmnI     ZraI
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