################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRII FauI FbaI FseI FspI HaeIII HgaI HincII HindII HindIII HpaI HphI KpnI KspI MaeI MamI MboI MflI MluI MluNI MroI MscI MseI MslI MspA1I MvaI MvnI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp45I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AclI 106 AACGTT AA^CGTT AcsI 324 AAATTT [GA]^AATT[CT] AcyI 83 GGCGCC G[GA]^CG[CT]C AflIII 493 ACATGT A^C[GA][CT]GT AluI 297 AGCT AG^CT Alw44I 436 GTGCAC G^TGCAC ApaLI 436 GTGCAC G^TGCAC ApoI 324 AAATTT [GA]^AATT[CT] AspHI 436 GTGCAC G[AT]GC[AT]^C AvaI 74 CCCGGG C^[CT]CG[GA]G AvaII 525 GGTCC G^G[AT]CC BanI 83 GGCGCC G^G[CT][GA]CC BbeI 83 GGCGCC GGCGC^C BglI 122 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 436 GTGCAC G[AGT]GC[ACT]^C BsaHI 83 GGCGCC G[GA]^CG[CT]C BsgI 330 CTGCAC GTGCAG(16/14) BsiHKAI 436 GTGCAC G[AT]GC[AT]^C BsmI 95 GAATGC GAATGC(1/-1) BsoBI 74 CCCGGG C^[CT]CG[GA]G Bsp1286I 436 GTGCAC G[AGT]GC[ACT]^C BspLU11I 493 ACATGT A^CATGT BstEII 559 GGTAACC G^GT[ACGT]ACC BstPI 559 GGTAACC G^GT[ACGT]ACC Cac8I 86 GCCAGC GC[ACGT]^[ACGT]GC CfoI 84 GCGC GCG^C Cfr13I 525 GGTCC G^G[ACGT]CC DdeI 144 CTCAG C^T[ACGT]AG EarI 231 CTCTTC CTCTTC(1/4) EcoNI 667 CCTAATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 559 GGTAACC G^GT[ACGT]ACC EcoRV 263 GATATC GAT^ATC EcoT22I 97 ATGCAT ATGCA^T HaeII 83 GGCGCC [GA]GCGC^[CT] HapII 75 CCGG C^CGG HhaI 84 GCGC GCG^C Hin1I 83 GGCGCC G[GA]^CG[CT]C HinP1I 84 GCGC G^CGC HpaII 75 CCGG C^CGG KasI 83 GGCGCC G^GCGCC Ksp632I 231 CTCTTC CTCTTC(1/4) MaeII 107 ACGT A^CGT MaeIII 560 GTAAC ^GT[ACGT]AC MfeI 381 CAATTG C^AATTG MspI 75 CCGG C^CGG MunI 381 CAATTG C^AATTG NarI 83 GGCGCC GG^CGCC NsiI 97 ATGCAT ATGCA^T PflMI 411 CCATACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 468 GAGTC GAGTC(4/5) Psp1406I 106 AACGTT AA^CGTT Sau96I 525 GGTCC G^G[ACGT]CC SfoI 83 GGCGCC GGC^GCC SmaI 74 CCCGGG CCC^GGG TaqI 261 TCGA T^CGA TthHB8I 261 TCGA T^CGA Van91I 411 CCATACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 74 CCCGGG C^CCGGG XmaI 74 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BcnI 74 CCCGG CC^[GC]GG 75 CCGGG BsmAI 583 GAGAC GTCTC(1/5) 227 GTCTC BsmFI 511 GGGAC GGGAC(10/14) 569 GTCCC BssKI 74 CCCGG ^CC[ACGT]GG 75 CCGGG NciI 74 CCCGG CC^[GC]GG 75 CCGGG NlaIV 83 GGCGCC GG[ACGT]^[ACGT]CC 524 GGGTCC NspI 139 ACATGC [GA]CATG^[CT] 493 ACATGT ScrFI 74 CCCGG CC^[ACGT]GG 75 CCGGG SfaNI 99 GCATC GCATC(5/9) 543 GCATC TfiI 459 GAATC G^A[AT]TC 715 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AfaI BbvI BsaJI BsiYI BslI Bsp1407I BsrGI BstF5I EcoT14I Fnu4HI FokI HinfI ItaI MboII MnlI MwoI NlaIII RsaI SspBI StyI TseI Tsp509I TspRI