Restriction map for pF1KB5399
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          451  GTAGAC               GT^[AC][GT]AC
AfaI           90  GTAC                 GT^AC
AlwNI         295  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI          54  GAGCAC               G[AT]GC[AT]^C
AvaII          42  GGACC                G^G[AT]CC
BanI          232  GGCACC               G^G[CT][GA]CC
BclI          404  TGATCA               T^GATCA
BmyI           54  GAGCAC               G[AGT]GC[ACT]^C
BsaJI         278  CCAGGG               C^C[ACGT][ACGT]GG
BsiHKAI        54  GAGCAC               G[AT]GC[AT]^C
BsmAI         176  GAGAC                GTCTC(1/5)
BsmBI         176  GAGACG               CGTCTC(1/5)
BsmFI         227  GGGAC                GGGAC(10/14)
Bsp1286I       54  GAGCAC               G[AGT]GC[ACT]^C
BspHI         407  TCATGA               T^CATGA
Cac8I         198  GCATGC               GC[ACGT]^[ACGT]GC
CfoI          260  GCGC                 GCG^C
DdeI          440  CTGAG                C^T[ACGT]AG
DraII          41  AGGACCT              [GA]G^G[ACGT]CC[CT]
EarI           51  GAAGAG               CTCTTC(1/4)
EcoO109I       41  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         508  GAATTC               G^AATTC
FbaI          404  TGATCA               T^GATCA
HhaI          260  GCGC                 GCG^C
HinP1I        260  GCGC                 G^CGC
Ksp632I        51  GAAGAG               CTCTTC(1/4)
MboII          51  GAAGA                GAAGA(8/7)
MseI           65  TTAA                 T^TAA
MslI          348  CACCCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          198  GCATGC               [GA]CATG^[CT]
PaeR7I        133  CTCGAG               C^TCGAG
PpuMI          41  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          553  CTGCAG               CTGCA^G
RcaI          407  TCATGA               T^CATGA
RsaI           90  GTAC                 GT^AC
SapI           51  GAAGAGC              GCTCTTC(1/4)
SexAI          44  ACCTGGT              A^CC[AT]GGT
SfaNI         360  GATGC                GCATC(5/9)
SfcI          553  CTGCAG               C^T[GA][CT]AG
SphI          198  GCATGC               GCATG^C
Tru9I          65  TTAA                 T^TAA
XhoI          133  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          139  GAATTT               [GA]^AATT[CT]
              508  GAATTC              
AcyI          178  GACGCC               G[GA]^CG[CT]C
              435  GACGCC              
ApoI          139  GAATTT               [GA]^AATT[CT]
              508  GAATTC              
AvaI          133  CTCGAG               C^[CT]CG[GA]G
              350  CCCGAG              
BpmI          276  CTCCAG               CTGGAG(16/14)
              364  CTGGAG              
BsaHI         178  GACGCC               G[GA]^CG[CT]C
              435  GACGCC              
BsoBI         133  CTCGAG               C^[CT]CG[GA]G
              350  CCCGAG              
HgaI          178  GACGC                GACGC(5/10)
              435  GACGC               
Hin1I         178  GACGCC               G[GA]^CG[CT]C
              435  GACGCC              
MwoI          193  GCCCAGCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              438  GCCTGAGCAGC         
NlaIV          24  GGCCCC               GG[ACGT]^[ACGT]CC
              232  GGCACC              
Tsp509I       140  AATT                 ^AATT
              509  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AluI        BbvI        BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HphI        ItaI        MboI        
MnlI        MvaI        NdeII       NlaIII      Sau3AI      
Sau96I      ScrFI       SmlI        TaqI        TseI        
TspRI       TthHB8I     
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