Restriction map for pF1KB5700
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        KasI        KpnI        
KspI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspI        StuI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AlwI          295  GATCC                GGATC(4/5)
Asp700I       757  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         730  GGTCC                G^G[AT]CC
AvrII         726  CCTAGG               C^CTAGG
BbsI           73  GAAGAC               GAAGAC(2/6)
BclI          863  TGATCA               T^GATCA
BcnI           26  CCGGG                CC^[GC]GG
BglII         699  AGATCT               A^GATCT
BlnI          726  CCTAGG               C^CTAGG
BpuAI          73  GAAGAC               GAAGAC(2/6)
BsaBI        1207  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          290  GTGCAG               GTGCAG(16/14)
BsmI          316  GCATTC               GAATGC(1/-1)
Bsp1407I     1007  TGTACA               T^GTACA
BsrGI        1007  TGTACA               T^GTACA
Cac8I         539  GCAAGC               GC[ACGT]^[ACGT]GC
Cfr13I        730  GGTCC                G^G[ACGT]CC
DdeI           20  CTCAG                C^T[ACGT]AG
DraIII        182  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I       726  CCTAGG               C^C[AT][AT]GG
FauI          935  CCCGC                CCCGC(4/6)
FbaI          863  TGATCA               T^GATCA
HaeIII         24  GGCC                 GG^CC
HapII          26  CCGG                 C^CGG
HincII        158  GTCAAC               GT[CT]^[GA]AC
HindII        158  GTCAAC               GT[CT]^[GA]AC
HindIII      1106  AAGCTT               A^AGCTT
HpaII          26  CCGG                 C^CGG
MaeII         171  ACGT                 A^CGT
MamI         1207  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI           26  CCGG                 C^CGG
NciI           26  CCGGG                CC^[GC]GG
NlaIV        1183  GGAGCC               GG[ACGT]^[ACGT]CC
NspI          614  GCATGT               [GA]CATG^[CT]
PleI          375  GACTC                GAGTC(4/5)
Sau96I        730  GGTCC                G^G[ACGT]CC
SpeI          858  ACTAGT               A^CTAGT
SspBI        1007  TGTACA               T^GTACA
StyI          726  CCTAGG               C^C[AT][AT]GG
TaqI           71  TCGA                 T^CGA
TthHB8I        71  TCGA                 T^CGA
XbaI          753  TCTAGA               T^CTAGA
XcmI          945  CCAGGGAACAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          757  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          338  GTAC                 GT^AC
             1008  GTAC                
BbvI           32  GCAGC                GCAGC(8/12)
              268  GCTGC               
BsiYI         594  CCCTGGAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              595  CCTGGAGGGGG         
BslI          594  CCCTGGAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              595  CCTGGAGGGGG         
BstYI         294  AGATCC               [GA]^GATC[CT]
              699  AGATCT              
EarI          670  GAAGAG               CTCTTC(1/4)
             1150  GAAGAG              
Fnu4HI         32  GCAGC                GC^[ACGT]GC
              268  GCTGC               
ItaI           32  GCAGC                GC^[ACGT]GC
              268  GCTGC               
Ksp632I       670  GAAGAG               CTCTTC(1/4)
             1150  GAAGAG              
MflI          294  AGATCC               [GA]^GATC[CT]
              699  AGATCT              
RsaI          338  GTAC                 GT^AC
             1008  GTAC                
SfaNI         415  GATGC                GCATC(5/9)
             1192  GATGC               
TseI           32  GCAGC                G^C[AT]GC
              268  GCTGC               
Tsp45I        136  GTCAC                ^GT[GC]AC
             1142  GTGAC               
XhoII         294  AGATCC               [GA]^GATC[CT]
              699  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BfaI        
BpmI        BsaJI       BsrI        BssKI       BstF5I      
BstNI       DpnII       EcoRII      FokI        HinfI       
HphI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      Sau3AI      ScrFI       SfcI        TfiI        
Tru9I       Tsp509I     TspRI       
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