Restriction map for
pF1KB5061
(Circular) (Six-base) MAPSORT of: pF1KB5061.seq Check: 3767 from: 1 to: 4529
pF1KB5061 4529 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1371 1567 1371
Size: 196 4333
Acc65I G'GTAC_C
Cuts at: 1545 1545
Size: 4529
AclI AA'CG_TT
Cuts at: 4369 4369
Size: 4529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2786 3533 3801 2354
Size: 432 747 268 3082
Fragments arranged by size:
3082 747 432 268
AflIII A'CryG_T
Cuts at: 1881 2985 3962 1881
Size: 1104 977 2448
Fragments arranged by size:
2448 1104 977
AgeI A'CCGG_T
Cuts at: 2917 2917
Size: 4529
AlwNI CAG_nnn'CTG
Cuts at: 904 1308 1834 3401 904
Size: 404 526 1567 2032
Fragments arranged by size:
2032 1567 526 404
ApaLI G'TGCA_C
Cuts at: 3299 3299
Size: 4529
ApoI r'AATT_y
Cuts at: 112 485 1533 3882 4138 112
Size: 373 1048 2349 256 503
Fragments arranged by size:
2349 1048 503 373 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 1549 1549
Size: 4529
AvrII C'CTAG_G
Cuts at: 1516 1516
Size: 4529
BamHI G'GATC_C
Cuts at: 1343 1437 1554 1343
Size: 94 117 4318
Fragments arranged by size:
4318 117 94
BanI G'GyrC_C
Cuts at: 1003 1132 1545 1959 2207 2242 1003
Size: 129 413 414 248 35 3290
Fragments arranged by size:
3290 414 413 248 129 35
BanII G_rGCy'C
Cuts at: 1543 2573 1543
Size: 1030 3499
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3188 4529 2427
Size: 761 1341 2427
Fragments arranged by size:
2427 1341 761
BclI T'GATC_A
Cuts at: 2049 2049
Size: 4529
BglII A'GATC_T
Cuts at: 1062 2044 4081 1062
Size: 982 2037 1510
Fragments arranged by size:
2037 1510 982
BlpI GC'TnA_GC
Cuts at: 1641 1641
Size: 4529
Bme1580I G_kGCm'C
Cuts at: 788 2154 2247 3303 788
Size: 1366 93 1056 2014
Fragments arranged by size:
2014 1366 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 453 944 1229 1741 1822 2157 4301 453
Size: 491 285 512 81 335 2144 681
Fragments arranged by size:
2144 681 512 491 335 285 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 442 496 518 1373 1382 427
Size: 15 54 22 855 9 3574
Fragments arranged by size:
3574 855 54 22 15 9
Bpu10I CC'TnA_GC
Cuts at: 1309 1309
Size: 4529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 1705 2584 3076 3374 633
Size: 1072 879 492 298 1788
Fragments arranged by size:
1788 1072 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4233 4233
Size: 4529
BsaAI yAC'GTr
Cuts at: 1882 1882
Size: 4529
BsaBI GATnn'nnATC
Cuts at: 6 499 2043 4080 6
Size: 493 1544 2037 455
Fragments arranged by size:
2037 1544 493 455
BsaHI Gr'CG_yC
Cuts at: 1960 2208 2910 4406 1960
Size: 248 702 1496 2083
Fragments arranged by size:
2083 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1718 2239 2917 3191 3338 3749 1718
Size: 521 678 274 147 411 2498
Fragments arranged by size:
2498 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 521 1279 521
Size: 758 3771
BseYI C'CCAG_C
Cuts at: 986 1954 2712 3289 3785 986
Size: 968 758 577 496 1730
Fragments arranged by size:
1730 968 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2071 2117 3325 104
Size: 1967 46 1208 1308
Fragments arranged by size:
1967 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 163 1103 1543 2321 2511 3303 163
Size: 940 440 778 190 792 1389
Fragments arranged by size:
1389 940 792 778 440 190
Bsp1286I G_dGCh'C
Cuts at: 163 788 1103 1543 2154 2247 2321 2511 2573
Size: 625 315 440 611 93 74 190 62
Cuts at: 2573 3303 163
Size: 730 1389
Fragments arranged by size:
1389 730 625 611 440 315 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3749 3749
Size: 4529
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 398 1579 1793 2095 2476 398
Size: 1181 214 302 381 2451
Fragments arranged by size:
2451 1181 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2821 2875 4359 4524 50
Size: 2771 54 1484 165 55
Fragments arranged by size:
2771 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2441 2441
Size: 4529
BsrFI r'CCGG_y
Cuts at: 2527 2708 2917 2527
Size: 181 209 4139
Fragments arranged by size:
4139 209 181
BssSI C'ACGA_G
Cuts at: 2800 3158 2800
Size: 358 4171
BstAPI GCAn_nnn'nTGC
Cuts at: 1906 1906
Size: 4529
BstBI TT'CG_AA
Cuts at: 2890 2890
Size: 4529
BstEII G'GTnAC_C
Cuts at: 564 564
Size: 4529
BstYI r'GATC_y
Cuts at: 1 292 1062 1343 1437 1554 2021 2044 2379
Size: 291 770 281 94 117 467 23 335
Cuts at: 2379 2625 3637 3723 3735 4081 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 770 467 449 346 335 291 281
246 117 94 86 23 12
BtgI C'CryG_G
Cuts at: 725 1007 1314 1413 725
Size: 282 307 99 3841
Fragments arranged by size:
3841 307 282 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 1245 1772 1245
Size: 527 4002
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3093 2235
Size: 858 3671
EaeI y'GGCC_r
Cuts at: 2114 2288 2679 2706 4051 2114
Size: 174 391 27 1345 2592
Fragments arranged by size:
2592 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2114 2114
Size: 4529
EarI CTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 3047 3193 4339 2737
Size: 310 146 1146 2927
Fragments arranged by size:
2927 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 442 496 518 1373 1382 2354 2786 3533
Size: 15 54 22 855 9 972 432 747
Cuts at: 3533 3801 427
Size: 268 1155
Fragments arranged by size:
1155 972 855 747 432 268 54 22
15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 536 755 536
Size: 219 4310
EcoO109I rG'GnC_Cy
Cuts at: 593 1305 1668 593
Size: 712 363 3454
Fragments arranged by size:
3454 712 363
EcoRI G'AATT_C
Cuts at: 485 1533 485
Size: 1048 3481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 860 1413 2548 860
Size: 553 1135 2841
Fragments arranged by size:
2841 1135 553
FspI TGC'GCA
Cuts at: 2310 2310
Size: 4529
HaeII r_GCGC'y
Cuts at: 1963 2211 3233 4212 1963
Size: 248 1022 979 2280
Fragments arranged by size:
2280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 930 962 2371 2401 2403 2433 930
Size: 32 1409 30 2 30 3026
Fragments arranged by size:
3026 1409 32 30 30 2
HincII GTy'rAC
Cuts at: 1568 1568
Size: 4529
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 1549 1549
Size: 4529
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 309 820 1196 3200 3384 309
Size: 511 376 2004 184 1454
Fragments arranged by size:
2004 1454 511 376 184
MscI TGG'CCA
Cuts at: 2290 2290
Size: 4529
MslI CAynn'nnrTG
Cuts at: 2518 2645 2518
Size: 127 4402
MspA1I CmG'CkG
Cuts at: 233 466 986 1640 1954 2314 3327 3572 233
Size: 233 520 654 314 360 1013 245 1190
Fragments arranged by size:
1190 1013 654 520 360 314 245 233
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NcoI C'CATG_G
Cuts at: 1007 1314 1007
Size: 307 4222
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NspI r_CATG'y
Cuts at: 1582 2082 2989 1582
Size: 500 907 3122
Fragments arranged by size:
3122 907 500
PciI A'CATG_T
Cuts at: 2985 2985
Size: 4529
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4497
PpuMI rG'GwC_Cy
Cuts at: 1305 1305
Size: 4529
PsiI TTA'TAA
Cuts at: 626 626
Size: 4529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 944 976 944
Size: 32 4497
PstI C_TGCA'G
Cuts at: 395 587 1113 1286 1576 2261 395
Size: 192 526 173 290 685 2663
Fragments arranged by size:
2663 685 526 290 192 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 233 466 986 1954 2314 233
Size: 233 520 968 360 2448
Fragments arranged by size:
2448 968 520 360 233
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SalI G'TCGA_C
Cuts at: 1566 1566
Size: 4529
SapI GCTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SfcI C'TryA_G
Cuts at: 33 391 583 744 920 1109 1176 1282 1572
Size: 358 192 161 176 189 67 106 290
Cuts at: 1572 2257 3250 3441 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 358 290 192 191 189
176 161 106 67
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 1551 1551
Size: 4529
SmlI C'TyrA_G
Cuts at: 648 1684 2599 3091 3353 648
Size: 1036 915 492 262 1824
Fragments arranged by size:
1824 1036 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1582 2082 1582
Size: 500 4029
SspI AAT'ATT
Cuts at: 1167 1167
Size: 4529
StyI C'CwwG_G
Cuts at: 692 1007 1314 1516 1663 2017 692
Size: 315 307 202 147 354 3204
Fragments arranged by size:
3204 354 315 307 202 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2112 2245 2704 2913 2112
Size: 133 459 209 3728
Fragments arranged by size:
3728 459 209 133
TatI w'GTAC_w
Cuts at: 960 4044 960
Size: 3084 1445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 136 168 4046 4078 136
Size: 32 3878 32 587
Fragments arranged by size:
3878 587 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1179 1257 1431 2024 1179
Size: 78 174 593 3684
Fragments arranged by size:
3684 593 174 78
XmaI C'CCGG_G
Cuts at: 1549 1549
Size: 4529
XmnI GAAnn'nnTTC
Cuts at: 843 843
Size: 4529
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI