Restriction map for pF1KB6254
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccI        AclI        AflII       AflIII      
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BamHI       BbrPI       BciVI       BclI        BfrI        
BglII       BlnI        BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
DraI        DraIII      Eam1105I    Eco47III    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KspI        MamI        
MluI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
SacII       SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Van91I      
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       1589  GGTACC               G^GTACC
AcsI          958  GAATTT               [GA]^AATT[CT]
AcyI         1208  GGCGCC               G[GA]^CG[CT]C
AgeI          233  ACCGGT               A^CCGGT
ApoI          958  GAATTT               [GA]^AATT[CT]
Asp700I       162  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1589  GGTACC               G^GTACC
AspI         1567  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1208  GGCGCC               GGCGC^C
BbsI         1990  GTCTTC               GAAGAC(2/6)
BbvCI         360  CCTCAGC              CCTCAGC(-5/-2)
BcgI           56  CGACCTCCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1034  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1034  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1990  GTCTTC               GAAGAC(2/6)
BsaAI        1996  TACGTG               [CT]AC^GT[GA]
BsaHI        1208  GGCGCC               G[GA]^CG[CT]C
BstAPI       1393  GCAGGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1034  GCTCAGC              GC^T[ACGT]AGC
CpoI         1906  CGGACCG              CG^G[AT]CCG
EcoT14I       326  CCTTGG               C^C[AT][AT]GG
Hin1I        1208  GGCGCC               G[GA]^CG[CT]C
HincII        948  GTCAAC               GT[CT]^[GA]AC
HindII        948  GTCAAC               GT[CT]^[GA]AC
KasI         1208  GGCGCC               G^GCGCC
KpnI         1589  GGTACC               GGTAC^C
MfeI          300  CAATTG               C^AATTG
MslI         1752  CATCCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          300  CAATTG               C^AATTG
NaeI         1188  GCCGGC               GCC^GGC
NarI         1208  GGCGCC               GG^CGCC
NgoMIV       1188  GCCGGC               G^CCGGC
PflFI        1567  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         233  ACCGGT               A^CCGGT
RsrII        1906  CGGACCG              CG^G[AT]CCG
SacI          508  GAGCTC               GAGCT^C
SbfI          684  CCTGCAGG             CCTGCA^GG
SfaNI         311  GATGC                GCATC(5/9)
SfoI         1208  GGCGCC               GGC^GCC
Sse8387I      684  CCTGCAGG             CCTGCA^GG
StyI          326  CCTTGG               C^C[AT][AT]GG
Tsp45I       2023  GTCAC                ^GT[GC]AC
Tth111I      1567  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1821  TCTAGA               T^CTAGA
XmnI          162  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII        566  TCCGGA               T^CCGGA
              977  TCCGGA              
AspHI         508  GAGCTC               G[AT]GC[AT]^C
             1863  GTGCTC              
BfaI          112  CTAG                 C^TAG
             1822  CTAG                
BseAI         566  TCCGGA               T^CCGGA
              977  TCCGGA              
BsgI           98  GTGCAG               GTGCAG(16/14)
             1054  GTGCAG              
BsiEI           8  CGGCCG               CG[GA][CT]^CG
             1732  CGGCCG              
BsiHKAI       508  GAGCTC               G[AT]GC[AT]^C
             1863  GTGCTC              
BsmBI         645  CGTCTC               CGTCTC(1/5)
               90  GAGACG              
BspEI         566  TCCGGA               T^CCGGA
              977  TCCGGA              
DrdI          556  GACCTCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1072  GACAGCCATGTC        
DsaI          122  CCGTGG               C^C[GA][CT]GG
             1483  CCACGG              
EagI            8  CGGCCG               C^GGCCG
             1732  CGGCCG              
EclXI           8  CGGCCG               C^GGCCG
             1732  CGGCCG              
Eco52I          8  CGGCCG               C^GGCCG
             1732  CGGCCG              
HaeII         155  GGCGCT               [GA]GCGC^[CT]
             1208  GGCGCC              
HgaI           92  GACGC                GACGC(5/10)
              644  GCGTC               
MaeI          112  CTAG                 C^TAG
             1822  CTAG                
MaeII         494  ACGT                 A^CGT
             1997  ACGT                
MaeIII        638  GTAAC                ^GT[ACGT]AC
             2023  GTCAC               
MroI          566  TCCGGA               T^CCGGA
              977  TCCGGA              
MseI         1135  TTAA                 T^TAA
             1618  TTAA                
SapI          595  GAAGAGC              GCTCTTC(1/4)
             1096  GAAGAGC             
TaqI         1070  TCGA                 T^CGA
             1833  TCGA                
TfiI          333  GAATC                G^A[AT]TC
              653  GAATC               
Tru9I        1135  TTAA                 T^TAA
             1618  TTAA                
TthHB8I      1070  TCGA                 T^CGA
             1833  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaI        BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        SmaI        SmlI        TseI        
Tsp509I     TspRI       XhoII       XmaCI       XmaI        
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