Restriction map for pF1KB6254
 (Linear) (Six-base) MAPSORT of: KIBB6254.seq  Check: 7083  from: 1  to: 2049



KIBB6254.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     692     735    1485    2049 
   Size:        692      43     750     564

  Fragments arranged by size: 

                 750     692     564      43
 
Acc65I G'GTAC_C

Cuts at:      0    1589    2049 
   Size:       1589     460
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763    2049 
   Size:        763    1286
 
AgeI A'CCGG_T

Cuts at:      0     233    2049 
   Size:        233    1816
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     730    2049 
   Size:        730    1319
 
AlwNI CAG_nnn'CTG

Cuts at:      0     845    1399    1477    1570    2049 
   Size:        845     554      78      93     479

  Fragments arranged by size: 

                 845     554     479      93      78
 
ApaI G_GGCC'C

Cuts at:      0     111    1013    1281    2049 
   Size:        111     902     268     768

  Fragments arranged by size: 

                 902     768     268     111
 
ApoI r'AATT_y

Cuts at:      0     958    2049 
   Size:        958    1091
 
AvaI C'yCGr_G

Cuts at:      0      67     173     241     250     831     995    1004    1084 
   Size:         67     106      68       9     581     164       9      80

Cuts at:   1084    1281    1895    2049 
   Size:        197     614     154

  Fragments arranged by size: 

                 614     581     197     164     154     106      80      68
                  67       9       9
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     870     903    2049 
   Size:        870      33    1146

  Fragments arranged by size: 

                1146     870      33
 
BanI G'GyrC_C

Cuts at:      0     246     466     680    1164    1208    1589    1845    2049 
   Size:        246     220     214     484      44     381     256     204

  Fragments arranged by size: 

                 484     381     256     246     220     214     204      44
            
 
BanII G_rGCy'C

Cuts at:      0     111     450     512    1004    1013    1281    1313    1419 
   Size:        111     339      62     492       9     268      32     106

Cuts at:   1419    1448    2049 
   Size:         29     601

  Fragments arranged by size: 

                 601     492     339     268     111     106      62      32
                  29       9
 
BbeI G_GCGC'C

Cuts at:      0    1212    2049 
   Size:       1212     837
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1983    2049 
   Size:       1983      66
 
BbvCI CC'TCA_GC

Cuts at:      0     361    2049 
   Size:        361    1688
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      45      79    2049 
   Size:         45      34    1970

  Fragments arranged by size: 

                1970      45      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16    1008    1795    1959    2049 
   Size:         16     992     787     164      90

  Fragments arranged by size: 

                 992     787     164      90      16
 
BlpI GC'TnA_GC

Cuts at:      0    1035    2049 
   Size:       1035    1014
 
Bme1580I G_kGCm'C

Cuts at:      0      68     111     251    1013    1167    1281    1748    1763 
   Size:         68      43     140     762     154     114     467      15

Cuts at:   1763    1791    1891    2049 
   Size:         28     100     158

  Fragments arranged by size: 

                 762     467     158     154     140     114     100      68
                  43      28      15
 
BmgBI CAC'GTC

Cuts at:      0     495    2049 
   Size:        495    1554
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     652    2049 
   Size:        652    1397
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1050    2049 
   Size:       1050     999
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     298    1867    1936    2049 
   Size:         59     239    1569      69     113

  Fragments arranged by size: 

                1569     239     113      69      59
 
Bpu10I CC'TnA_GC

Cuts at:      0     286     361    1312    1548    2049 
   Size:        286      75     951     236     501

  Fragments arranged by size: 

                 951     501     286     236      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1334    1663    1777    2049 
   Size:       1334     329     114     272

  Fragments arranged by size: 

                1334     329     272     114
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      77    1040    1500    1572    2049 
   Size:         77     963     460      72     477

  Fragments arranged by size: 

                 963     477     460      77      72
 
BsaAI yAC'GTr

Cuts at:      0    1998    2049 
   Size:       1998      51
 
BsaHI Gr'CG_yC

Cuts at:      0    1209    2049 
   Size:       1209     840
 
BsaWI w'CCGG_w

Cuts at:      0     233     566     977    2049 
   Size:        233     333     411    1072

  Fragments arranged by size: 

                1072     411     333     233
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      81     111     631     661    1037    1067    2049 
   Size:         81      30     520      30     376      30     982

  Fragments arranged by size: 

                 982     520     376      81      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     130    1129    1198    1213    1524    1870    2049 
   Size:         55      75     999      69      15     311     346     179

  Fragments arranged by size: 

                 999     346     311     179      75      69      55      15
            
 
BseYI C'CCAG_C

Cuts at:      0     321     441     600    2049 
   Size:        321     120     159    1449

  Fragments arranged by size: 

                1449     321     159     120
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1075    2049 
   Size:        119     956     974

  Fragments arranged by size: 

                 974     956     119
 
BsiEI CG_ry'CG

Cuts at:      0      11    1735    2049 
   Size:         11    1724     314

  Fragments arranged by size: 

                1724     314      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     512    1867    2049 
   Size:        512    1355     182

  Fragments arranged by size: 

                1355     512     182
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      84     651    2049 
   Size:         84     567    1398

  Fragments arranged by size: 

                1398     567      84
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     111     251     450     512    1004    1013    1167 
   Size:         68      43     140     199      62     492       9     154

Cuts at:   1167    1281    1313    1419    1448    1748    1763    1791    1867 
   Size:        114      32     106      29     300      15      28      76

Cuts at:   1867    1891    2049 
   Size:         24     158

  Fragments arranged by size: 

                 492     300     199     158     154     140     114     106
                  76      68      62      43      32      29      28      24
                  15       9
 
BspEI T'CCGG_A

Cuts at:      0     566     977    2049 
   Size:        566     411    1072

  Fragments arranged by size: 

                1072     566     411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     678     692     735    1485    2049 
   Size:        678      14      43     750     564

  Fragments arranged by size: 

                 750     678     564      43      14
 
BsrBI CCG'CTC

Cuts at:      0     105    1203    1239    1265    2049 
   Size:        105    1098      36      26     784

  Fragments arranged by size: 

                1098     784     105      36      26
 
BsrFI r'CCGG_y

Cuts at:      0     152     233    1188    2049 
   Size:        152      81     955     861

  Fragments arranged by size: 

                 955     861     152      81
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1399    2049 
   Size:       1399     650
 
BstYI r'GATC_y

Cuts at:      0     858     980    1875    2049 
   Size:        858     122     895     174

  Fragments arranged by size: 

                 895     858     174     122
 
Bsu36I CC'TnA_GG

Cuts at:      0     412     676    1670    1850    2049 
   Size:        412     264     994     180     199

  Fragments arranged by size: 

                 994     412     264     199     180
 
BtgI C'CryG_G

Cuts at:      0     122    1483    2049 
   Size:        122    1361     566

  Fragments arranged by size: 

                1361     566     122
 
BtsI GCAGTG_nn'

Cuts at:      0     102    1889    2049 
   Size:        102    1787     160

  Fragments arranged by size: 

                1787     160     102
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     562    1078    2049 
   Size:        562     516     971

  Fragments arranged by size: 

                 971     562     516
 
EaeI y'GGCC_r

Cuts at:      0       8     125    1486    1679    1691    1718    1732    2049 
   Size:          8     117    1361     193      12      27      14     317

  Fragments arranged by size: 

                1361     317     193     117      27      14      12       8
            
 
EagI C'GGCC_G

Cuts at:      0       8    1732    2049 
   Size:          8    1724     317

  Fragments arranged by size: 

                1724     317       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     549     590    1091    1528    2049 
   Size:        549      41     501     437     521

  Fragments arranged by size: 

                 549     521     501     437      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     610    2049 
   Size:        610    1439
 
EcoICRI GAG'CTC

Cuts at:      0     510    2049 
   Size:        510    1539
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     298     763    1867    1936    2049 
   Size:         59     239     465    1104      69     113

  Fragments arranged by size: 

                1104     465     239     113      69      59
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115    1145    1669    1681    2049 
   Size:        115    1030     524      12     368

  Fragments arranged by size: 

                1030     524     368     115      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108     134     282     417     555     606     810    1009 
   Size:        108      26     148     135     138      51     204     199

Cuts at:   1009    1277    1278    1552    1638    1674    1944    2049 
   Size:        268       1     274      86      36     270     105

  Fragments arranged by size: 

                 274     270     268     204     199     148     138     135
                 108     105      86      51      36      26       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1465    2049 
   Size:       1465     584
 
HaeII r_GCGC'y

Cuts at:      0     159    1212    2049 
   Size:        159    1053     837

  Fragments arranged by size: 

                1053     837     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     499     531     825     857    1849    1881    2049 
   Size:        499      32     294      32     992      32     168

  Fragments arranged by size: 

                 992     499     294     168      32      32      32
 
HincII GTy'rAC

Cuts at:      0     950    2049 
   Size:        950    1099
 
KasI G'GCGC_C

Cuts at:      0    1208    2049 
   Size:       1208     841
 
KpnI G_GTAC'C

Cuts at:      0    1593    2049 
   Size:       1593     456
 
MfeI C'AATT_G

Cuts at:      0     300    2049 
   Size:        300    1749
 
MscI TGG'CCA

Cuts at:      0     127    1693    1720    2049 
   Size:        127    1566      27     329

  Fragments arranged by size: 

                1566     329     127      27
 
MslI CAynn'nnrTG

Cuts at:      0    1756    2049 
   Size:       1756     293
 
MspA1I CmG'CkG

Cuts at:      0     267     375     405    1039    1227    1405    1629    1659 
   Size:        267     108      30     634     188     178     224      30

Cuts at:   1659    1716    2049 
   Size:         57     333

  Fragments arranged by size: 

                 634     333     267     224     188     178     108      57
                  30      30
 
NaeI GCC'GGC

Cuts at:      0    1190    2049 
   Size:       1190     859
 
NarI GG'CG_CC

Cuts at:      0    1209    2049 
   Size:       1209     840
 
NgoMIV G'CCGG_C

Cuts at:      0    1188    2049 
   Size:       1188     861
 
PasI CC'CwG_GG

Cuts at:      0     471     804    1141    2049 
   Size:        471     333     337     908

  Fragments arranged by size: 

                 908     471     337     333
 
PfoI T'CCnGG_A

Cuts at:      0     226     867    2049 
   Size:        226     641    1182

  Fragments arranged by size: 

                1182     641     226
 
PpuMI rG'GwC_Cy

Cuts at:      0     134     417     555     606     810    1638    1674    2049 
   Size:        134     283     138      51     204     828      36     375

  Fragments arranged by size: 

                 828     375     283     204     138     134      51      36
            
 
PspOMI G'GGCC_C

Cuts at:      0     107    1009    1277    2049 
   Size:        107     902     268     772

  Fragments arranged by size: 

                 902     772     268     107
 
PstI C_TGCA'G

Cuts at:      0     689     719     855    1404    1658    2049 
   Size:        689      30     136     549     254     391

  Fragments arranged by size: 

                 689     549     391     254     136      30
 
PvuII CAG'CTG

Cuts at:      0     267     375     405    1227    1629    1716    2049 
   Size:        267     108      30     822     402      87     333

  Fragments arranged by size: 

                 822     402     333     267     108      87      30
 
RsrII CG'GwC_CG

Cuts at:      0    1907    2049 
   Size:       1907     142
 
SacI G_AGCT'C

Cuts at:      0     512    2049 
   Size:        512    1537
 
SanDI GG'GwC_CC

Cuts at:      0     134    2049 
   Size:        134    1915
 
SapI GCTCTTCn'nnn_

Cuts at:      0     590    1091    2049 
   Size:        590     501     958

  Fragments arranged by size: 

                 958     590     501
 
SbfI CC_TGCA'GG

Cuts at:      0     689    2049 
   Size:        689    1360
 
SfcI C'TryA_G

Cuts at:      0     685     715     851    1400    1654    2049 
   Size:        685      30     136     549     254     395

  Fragments arranged by size: 

                 685     549     395     254     136      30
 
SfoI GGC'GCC

Cuts at:      0    1210    2049 
   Size:       1210     839
 
SmaI CCC'GGG

Cuts at:      0      69     175     997    1006    1086    1897    2049 
   Size:         69     106     822       9      80     811     152

  Fragments arranged by size: 

                 822     811     152     106      80      69       9
 
SmlI C'TyrA_G

Cuts at:      0    1349    1642    1792    2049 
   Size:       1349     293     150     257

  Fragments arranged by size: 

                1349     293     257     150
 
StyI C'CwwG_G

Cuts at:      0     326    2049 
   Size:        326    1723
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226    2049 
   Size:        226    1823
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      81     113    1037    1069    2049 
   Size:         81      32     924      32     980

  Fragments arranged by size: 

                 980     924      81      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1570    2049 
   Size:       1570     479
 
XbaI T'CTAG_A

Cuts at:      0    1821    2049 
   Size:       1821     228
 
XmaI C'CCGG_G

Cuts at:      0      67     173     995    1004    1084    1895    2049 
   Size:         67     106     822       9      80     811     154

  Fragments arranged by size: 

                 822     811     154     106      80      67       9
 
XmnI GAAnn'nnTTC

Cuts at:      0     166    2049 
   Size:        166    1883
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AgeI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BstAPI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     PasI     PfoI    PpuMI   PspOMI
     PstI    PvuII    RsrII     SacI    SanDI     SapI     SbfI     SfcI
     SfoI     SmaI     SmlI     StyI    TaqII     TstI  Tth111I     XbaI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI    BciVI
     BclI    BfrBI    BglII     BmtI    BsaBI    BsiWI     BsmI    BspHI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     MluI     MmeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    SacII     SalI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XcmI
     XhoI     ZraI
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