Restriction map for pF1KB4899
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BpuAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MslI        MunI        MvnI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1296  GACGTC               GACGT^C
AflIII       1313  ACGTGT               A^C[GA][CT]GT
AlwNI         409  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1307  AGCGCT               AGC^GCT
AspHI         861  GAGCTC               G[AT]GC[AT]^C
AvaI         1360  CCCGAG               C^[CT]CG[GA]G
BalI          405  TGGCCA               TGG^CCA
BanII         861  GAGCTC               G[GA]GC[CT]^C
BclI          497  TGATCA               T^GATCA
BglI           37  GCCCCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          861  GAGCTC               G[AGT]GC[ACT]^C
BsaAI        1312  TACGTG               [CT]AC^GT[GA]
BsiHKAI       861  GAGCTC               G[AT]GC[AT]^C
BsmBI        1157  GAGACG               CGTCTC(1/5)
BsoBI        1360  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      861  GAGCTC               G[AGT]GC[ACT]^C
BspHI         180  TCATGA               T^CATGA
BspMI        1047  ACCTGC               ACCTGC(4/8)
BstBI         940  TTCGAA               TT^CGAA
DraII         100  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI          133  GAAGAG               CTCTTC(1/4)
Eco47III     1307  AGCGCT               AGC^GCT
EcoNI         423  CCTTGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      100  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       423  CCTTGG               C^C[AT][AT]GG
FauI         1199  CCCGC                CCCGC(4/6)
FbaI          497  TGATCA               T^GATCA
HincII        210  GTTAAC               GT[CT]^[GA]AC
HindII        210  GTTAAC               GT[CT]^[GA]AC
HpaI          210  GTTAAC               GTT^AAC
Ksp632I       133  GAAGAG               CTCTTC(1/4)
MluNI         405  TGGCCA               TGG^CCA
MscI          405  TGGCCA               TGG^CCA
NaeI         1063  GCCGGC               GCC^GGC
NgoMIV       1063  GCCGGC               G^CCGGC
NspI          659  GCATGT               [GA]CATG^[CT]
NspV          940  TTCGAA               TT^CGAA
PpuMI         100  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          180  TCATGA               T^CATGA
SacI          861  GAGCTC               GAGCT^C
SapI          133  GAAGAGC              GCTCTTC(1/4)
SexAI          29  ACCAGGT              A^CC[AT]GGT
SfaNI         411  GATGC                GCATC(5/9)
SfuI          940  TTCGAA               TT^CGAA
SgrAI        1201  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI           67  CTCAAG               C^T[CT][GA]AG
StyI          423  CCTTGG               C^C[AT][AT]GG
XcmI          298  CCAAAGAACCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          540  AAATTC               [GA]^AATT[CT]
              789  GAATTT              
AfaI          685  GTAC                 GT^AC
             1317  GTAC                
ApoI          540  AAATTC               [GA]^AATT[CT]
              789  GAATTT              
Asp700I         3  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              433  GAACGCTTTC          
BbeI          284  GGCGCC               GGCGC^C
              606  GGCGCC              
BcnI         1020  CCCGG                CC^[GC]GG
             1254  CCGGG               
BlpI          729  GCTCAGC              GC^T[ACGT]AGC
             1248  GCTGAGC             
Bpu1102I      729  GCTCAGC              GC^T[ACGT]AGC
             1248  GCTGAGC             
BseRI         874  CTCCTC               GAGGAG(10/8)
             1011  GAGGAG              
BsgI         1369  CTGCAC               GTGCAG(16/14)
              219  GTGCAG              
BsmI          617  GAATGC               GAATGC(1/-1)
              293  GCATTC              
BsrFI        1063  GCCGGC               [GA]^CCGG[CT]
             1202  GCCGGT              
BstAPI        138  GCAAACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              147  GCAAGAAGTGC         
CelII         729  GCTCAGC              GC^T[ACGT]AGC
             1248  GCTGAGC             
Cfr10I       1063  GCCGGC               [GA]^CCGG[CT]
             1202  GCCGGT              
DpnII         498  GATC                 ^GATC
              526  GATC                
EaeI          405  TGGCCA               [CT]^GGCC[GA]
             1061  TGGCCG              
KasI          284  GGCGCC               G^GCGCC
              606  GGCGCC              
MboI          498  GATC                 ^GATC
              526  GATC                
NarI          284  GGCGCC               GG^CGCC
              606  GGCGCC              
NciI         1020  CCCGG                CC^[GC]GG
             1254  CCGGG               
NdeII         498  GATC                 ^GATC
              526  GATC                
RsaI          685  GTAC                 GT^AC
             1317  GTAC                
Sau3AI        498  GATC                 ^GATC
              526  GATC                
SfoI          284  GGCGCC               GGC^GCC
              606  GGCGCC              
XmnI            3  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              433  GAACGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BanI        
BbvI        BpmI        BsaHI       BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NlaIII      
NlaIV       PleI        PstI        PvuII       Sau96I      
ScrFI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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