Restriction map for pF1KB4899
 (Linear) (Six-base) MAPSORT of: KIBB4899.seq  Check: 3405  from: 1  to: 1407



KIBB4899.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AatII G_ACGT'C

Cuts at:      0    1300    1407 
   Size:       1300     107
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243    1407 
   Size:        243    1164
 
AfeI AGC'GCT

Cuts at:      0    1309    1407 
   Size:       1309      98
 
AflIII A'CryG_T

Cuts at:      0    1313    1407 
   Size:       1313      94
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414    1407 
   Size:        414     993
 
ApoI r'AATT_y

Cuts at:      0     540     789    1407 
   Size:        540     249     618

  Fragments arranged by size: 

                 618     540     249
 
AvaI C'yCGr_G

Cuts at:      0    1360    1407 
   Size:       1360      47
 
BanI G'GyrC_C

Cuts at:      0     284     450     606    1407 
   Size:        284     166     156     801

  Fragments arranged by size: 

                 801     284     166     156
 
BanII G_rGCy'C

Cuts at:      0     865    1407 
   Size:        865     542
 
BbeI G_GCGC'C

Cuts at:      0     288     610    1407 
   Size:        288     322     797

  Fragments arranged by size: 

                 797     322     288
 
BclI T'GATC_A

Cuts at:      0     497    1407 
   Size:        497     910
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43    1407 
   Size:         43    1364
 
BlpI GC'TnA_GC

Cuts at:      0     730    1249    1407 
   Size:        730     519     158

  Fragments arranged by size: 

                 730     519     158
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      82    1407 
   Size:         82    1325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     508     862    1129    1175    1321    1407 
   Size:        463      45     354     267      46     146      86

  Fragments arranged by size: 

                 463     354     267     146      86      46      45
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1407 
   Size:         52    1355
 
BsaAI yAC'GTr

Cuts at:      0    1314    1407 
   Size:       1314      93
 
BsaHI Gr'CG_yC

Cuts at:      0     285     607     814    1297    1385    1407 
   Size:        285     322     207     483      88      22

  Fragments arranged by size: 

                 483     322     285     207      88      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     538     568     814     844    1407 
   Size:        538      30     246      30     563

  Fragments arranged by size: 

                 563     538     246      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     865    1026    1407 
   Size:        865     161     381

  Fragments arranged by size: 

                 865     381     161
 
BseYI C'CCAG_C

Cuts at:      0     679    1347    1407 
   Size:        679     668      60

  Fragments arranged by size: 

                 679     668      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    1354    1407 
   Size:        240    1114      53

  Fragments arranged by size: 

                1114     240      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     865    1407 
   Size:        865     542
 
BsmI GAATG_Cn'

Cuts at:      0     293     623    1407 
   Size:        293     330     784

  Fragments arranged by size: 

                 784     330     293
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1151    1407 
   Size:       1151     256
 
Bsp1286I G_dGCh'C

Cuts at:      0     865    1407 
   Size:        865     542
 
BspHI T'CATG_A

Cuts at:      0     180    1407 
   Size:        180    1227
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1056    1407 
   Size:       1056     351
 
BsrFI r'CCGG_y

Cuts at:      0    1063    1202    1407 
   Size:       1063     139     205

  Fragments arranged by size: 

                1063     205     139
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     144     153    1407 
   Size:        144       9    1254

  Fragments arranged by size: 

                1254     144       9
 
BstBI TT'CG_AA

Cuts at:      0     941    1407 
   Size:        941     466
 
BtsI GCAGTG_nn'

Cuts at:      0     142    1407 
   Size:        142    1265
 
EaeI y'GGCC_r

Cuts at:      0     405    1061    1407 
   Size:        405     656     346

  Fragments arranged by size: 

                 656     405     346
 
EarI CTCTTCn'nnn_

Cuts at:      0     128    1407 
   Size:        128    1279
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     177    1407 
   Size:        177    1230
 
EcoICRI GAG'CTC

Cuts at:      0     863    1407 
   Size:        863     544
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     463     508     862    1129    1175    1321    1407 
   Size:        243     220      45     354     267      46     146      86

  Fragments arranged by size: 

                 354     267     243     220     146      86      46      45
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     427    1407 
   Size:        427     980
 
EcoO109I rG'GnC_Cy

Cuts at:      0     101    1407 
   Size:        101    1306
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     453    1407 
   Size:        453     954
 
HaeII r_GCGC'y

Cuts at:      0      75     288     610    1311    1407 
   Size:         75     213     322     701      96

  Fragments arranged by size: 

                 701     322     213      96      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     510     542     814     846    1102    1134    1407 
   Size:        510      32     272      32     256      32     273

  Fragments arranged by size: 

                 510     273     272     256      32      32      32
 
HincII GTy'rAC

Cuts at:      0     212    1407 
   Size:        212    1195
 
HpaI GTT'AAC

Cuts at:      0     212    1407 
   Size:        212    1195
 
KasI G'GCGC_C

Cuts at:      0     284     606    1407 
   Size:        284     322     801

  Fragments arranged by size: 

                 801     322     284
 
MscI TGG'CCA

Cuts at:      0     407    1407 
   Size:        407    1000
 
MspA1I CmG'CkG

Cuts at:      0     282    1054    1092    1148    1407 
   Size:        282     772      38      56     259

  Fragments arranged by size: 

                 772     282     259      56      38
 
NaeI GCC'GGC

Cuts at:      0    1065    1407 
   Size:       1065     342
 
NarI GG'CG_CC

Cuts at:      0     285     607    1407 
   Size:        285     322     800

  Fragments arranged by size: 

                 800     322     285
 
NgoMIV G'CCGG_C

Cuts at:      0    1063    1407 
   Size:       1063     344
 
NspI r_CATG'y

Cuts at:      0     663    1407 
   Size:        663     744
 
PfoI T'CCnGG_A

Cuts at:      0     650     883    1106    1407 
   Size:        650     233     223     301

  Fragments arranged by size: 

                 650     301     233     223
 
PpuMI rG'GwC_Cy

Cuts at:      0     101    1407 
   Size:        101    1306
 
PstI C_TGCA'G

Cuts at:      0     389     955    1053    1407 
   Size:        389     566      98     354

  Fragments arranged by size: 

                 566     389     354      98
 
PvuII CAG'CTG

Cuts at:      0    1054    1092    1148    1407 
   Size:       1054      38      56     259

  Fragments arranged by size: 

                1054     259      56      38
 
SacI G_AGCT'C

Cuts at:      0     865    1407 
   Size:        865     542
 
SanDI GG'GwC_CC

Cuts at:      0     101    1407 
   Size:        101    1306
 
SapI GCTCTTCn'nnn_

Cuts at:      0     128    1407 
   Size:        128    1279
 
SexAI A'CCwGG_T

Cuts at:      0      29    1407 
   Size:         29    1378
 
SfcI C'TryA_G

Cuts at:      0     385     951    1049    1407 
   Size:        385     566      98     358

  Fragments arranged by size: 

                 566     385     358      98
 
SfoI GGC'GCC

Cuts at:      0     286     608    1407 
   Size:        286     322     799

  Fragments arranged by size: 

                 799     322     286
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1202    1407 
   Size:       1202     205
 
SmlI C'TyrA_G

Cuts at:      0      67    1407 
   Size:         67    1340
 
StyI C'CwwG_G

Cuts at:      0     423    1407 
   Size:        423     984
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454     693    1407 
   Size:        454     239     714

  Fragments arranged by size: 

                 714     454     239
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     538     543     570     575     646     678    1407 
   Size:        538       5      27       5      71      32     729

  Fragments arranged by size: 

                 729     538      71      32      27       5       5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     305    1407 
   Size:        305    1102
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     437    1407 
   Size:          7     430     970

  Fragments arranged by size: 

                 970     430       7
 
ZraI GAC'GTC

Cuts at:      0    1298    1407 
   Size:       1298     109
 

 Enzymes that do cut:

    AatII     AcuI     AfeI   AflIII    AlwNI     ApoI     AvaI     BanI
    BanII     BbeI     BclI     BglI     BlpI     BplI     BpmI    BpuEI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrFI   BstAPI    BstBI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     HpaI     KasI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PfoI    PpuMI     PstI    PvuII     SacI
    SanDI     SapI    SexAI     SfcI     SfoI    SgrAI     SmlI     StyI
    TaqII     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaBI    BsaWI
    BsiEI    BsiWI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     KpnI     MfeI     MluI     MmeI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
     SfiI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
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