################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 982 GTCTAC GT^[AC][GT]AC AccII 723 CGCG CG^CG AcyI 513 GACGCC G[GA]^CG[CT]C AflIII 287 ACGTGT A^C[GA][CT]GT AlwI 499 GATCC GGATC(4/5) AspHI 111 GAGCTC G[AT]GC[AT]^C AvaI 194 CCCGAG C^[CT]CG[GA]G BcgI 269 GCACAGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 103 GTATCC GTATCC(6/5) BglI 574 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 513 GACGCC G[GA]^CG[CT]C BsiHKAI 111 GAGCTC G[AT]GC[AT]^C BsoBI 194 CCCGAG C^[CT]CG[GA]G BspHI 482 TCATGA T^CATGA BspMI 298 ACCTGC ACCTGC(4/8) BsrI 857 CCAGT ACTGG(1/-1) BstEII 294 GGTCACC G^GT[ACGT]ACC BstF5I 410 CATCC GGATG(2/0) BstPI 294 GGTCACC G^GT[ACGT]ACC BstUI 723 CGCG CG^CG BstYI 498 AGATCC [GA]^GATC[CT] CfoI 58 GCGC GCG^C DrdI 556 GACAACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 299 CCTGCAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 294 GGTCACC G^GT[ACGT]ACC FokI 410 CATCC GGATG(9/13) HaeII 57 GGCGCT [GA]GCGC^[CT] HhaI 58 GCGC GCG^C Hin1I 513 GACGCC G[GA]^CG[CT]C HinP1I 58 GCGC G^CGC HinfI 610 GACTC G^A[ACGT]TC MaeII 287 ACGT A^CGT MflI 498 AGATCC [GA]^GATC[CT] MvnI 723 CGCG CG^CG NaeI 65 GCCGGC GCC^GGC NgoMIV 65 GCCGGC G^CCGGC PleI 610 GACTC GAGTC(4/5) PpuMI 683 GGGACCC [GA]G^G[AT]CC[CT] RcaI 482 TCATGA T^CATGA SacI 111 GAGCTC GAGCT^C ScaI 221 AGTACT AGT^ACT SexAI 236 ACCTGGT A^CC[AT]GGT SfaNI 999 GATGC GCATC(5/9) SfiI 573 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 278 TCGA T^CGA Tsp509I 165 AATT ^AATT TthHB8I 278 TCGA T^CGA XcmI 413 CCAATGGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 498 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 684 GGACC G^G[AT]CC 777 GGTCC BcnI 441 CCGGG CC^[GC]GG 1014 CCCGG BsmAI 170 GAGAC GTCTC(1/5) 628 GAGAC BsrFI 65 GCCGGC [GA]^CCGG[CT] 736 GCCGGT Cfr10I 65 GCCGGC [GA]^CCGG[CT] 736 GCCGGT DraII 581 AGGCCCC [GA]G^G[ACGT]CC[CT] 683 GGGACCC EarI 643 GAAGAG CTCTTC(1/4) 648 GAAGAG EcoO109I 581 AGGCCCC [GA]G^G[ACGT]CC[CT] 683 GGGACCC EcoT14I 707 CCAAGG C^C[AT][AT]GG 863 CCAAGG HgaI 513 GACGC GACGC(5/10) 743 GCGTC Ksp632I 643 GAAGAG CTCTTC(1/4) 648 GAAGAG NciI 441 CCGGG CC^[GC]GG 1014 CCCGG PvuII 257 CAGCTG CAG^CTG 523 CAGCTG SfcI 264 CTGTAG C^T[GA][CT]AG 923 CTACAG StyI 707 CCAAGG C^C[AT][AT]GG 863 CCAAGG TspRI 561 CACTG CA[GC]TG[ACGT][ACGT]^ 873 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI BalI BanI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstXI Cac8I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I